Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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In vitro anti-oxidant, anti-wrinkle and whitening effect of solvent fraction from takli-san

탁리산 용매 분획물의 in vitro 항산화, 주름 억제 및 미백 효과

  • Yu, Jae-Myo (National Development Institute of Korean Medicine) ;
  • Kang, Yun-Hwan (National Development Institute of Korean Medicine) ;
  • Kim, Dong-Hee (National Development Institute of Korean Medicine) ;
  • Park, Tae-Soon (National Development Institute of Korean Medicine)
  • Received : 2017.06.22
  • Accepted : 2017.07.24
  • Published : 2017.09.29
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Abstract

In this study, the activity of Takli-san for anti-oxidation, anti-wrinkle and whitening effect was verified and its applicability as a cosmetic material was confirmed. 1-1-diphenyl-2-picryl-hydrazyl radical scavenge, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenge, elastase and tyrosinase inhibitory activity were investigated by solvent fraction, and ethyl acetate fraction of water extract from Takli-san (TW-EA) showed the highest inhibitory activity. Western blot was performed to confirm anti-wrinkle and whitening effect in the cells, and it was investigeted that the TW-EA inhibition effect was superior to that of the positive contol used for the comparison test. Through the results of the experiments, the applicability as a cosmetic material of Takli-san was verified.

본 연구에서는 탁리산 열수추출물을 용매 분획하여 항산화, 주름억제, 미백효과에 대한 활성을 검증하여 화장품 소재로서 이용가능성을 연구하였다. 용매 분획물로 DPPH radical scavenge, ABTS radical scavenge, elastase, tyrosinase 저해활성을 확인하였고 에틸아세테이트 분획물(TW-EA)이 가장 높은 저해활성을 나타내었다. 세포내에서의 주름억제, 미백효과를 확인하기 위해서 western blot을 실시하였고 비교 검증을 위해 사용한 positive contol보다 TW-EA의 억제효과가 뛰어난 것을 확인하였다. 이러한 결과로 탁리산의 화장품소재로서 적용 가능성을 검증하였다.

Keywords

References

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