Applied Microscopy

  • 제47권3호
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  • Pages.171-175
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  • 2017
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  • 2287-5123(pISSN)
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  • 2287-4445(eISSN)
한국현미경학회 (Korean Society of Microscopy)
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Structural Analysis of Exosomes Using Different Types of Electron Microscopy

  • Choi, Hyosun (BK21 Plus Program, Department of Senior Healthcare, Graduate School, Eulji University) ;
  • Mun, Ji Young (BK21 Plus Program, Department of Senior Healthcare, Graduate School, Eulji University)
  • 투고 : 2017.06.12
  • 심사 : 2017.07.24
  • 발행 : 2017.09.30
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초록

Negative staining has been traditionally used for exosome imaging; however, the technique is limited to surface topology only and can cause staining artifacts. Therefore, to analyze the internal structure of exosomes, we employed a method of block preparation, thin sectioning, and electron tomography. In addition, an automatic serial sectioning technique with 15-nm thickness through focused ion beam was employed to observe the three-dimensional structure of exosomes of various sizes. Cryo-transmission electron microscopy revealed the near-to-native structure of exosomes.

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참고문헌

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