Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Genotoxicity Studies on Corn Silk Extract Containing High Maysin

고메이신 함유 옥수수수염 추출물의 유전독성학적 안전성 연구

  • Ha, Ae Wha (Department of Food Science and Nutrition, Dankook University) ;
  • Kang, Hyeon Jung (Crop Foundation Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Sun Lim (Crop Post-Harvest Technology Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Myung Hwan (Department of Food Engineering, Dankook University) ;
  • Kim, Woo Kyoung (Department of Food Science and Nutrition, Dankook University)
  • 하애화 (단국대학교 자연과학대학 식품영양학과) ;
  • 강현중 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 김선림 (농촌진흥청 국립식량과학원 중부작물부, 수확후이용과) ;
  • 김명환 (단국대학교 융합기술대학 식품공학과) ;
  • 김우경 (단국대학교 자연과학대학 식품영양학과)
  • Received : 2017.03.02
  • Accepted : 2017.05.24
  • Published : 2017.09.30
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Abstract

In this study, a battery of genetic-toxicity studies on corn silk extract with high maysin content were performed according to internationally accepted protocols. In a mutation test using Salmonella Typhimurium TA1535, TA1537, TA98, and TA100, the number of mutant colonies did not significantly increase up to a maximum concentration of $5,000{\mu}g/plate$ in the presence or absence of the S9 metabolic activation system. In the chromosome aberration test using Chinese hamster lung fibroblasts, negative results were observed in the concentration up to $1,250{\mu}g/mL$ of corn silk extract. In the micronucleus test using ICR mice, incidence of polymorphonuclear erythrocytes with a maximum concentration of 2,000 mg/kg corn silk extract did not show any significant difference compared to the negative control group. Based on these results, the test substance, con silk extract, did not influence genotoxicity.

본 연구에서는 고메이신 함유 옥수수수염 추출물의 유전독성에 대한 안전성을 규명하고자 세균에서의 복귀돌연변이 유발성, 염색체 이상, 마우스 골수세포에 있어서 소핵실험을 수행하였다. 세균에서의 복귀돌연변이 유발성 여부는 Salmonella Typhimurium의 히스티딘 요구성 균주인 TA100, TA1535, TA98 및 TA1537의 4개 균주와 대장균 Escherichia coli의 트립토판 요구성 균주인 WP2 uvrA를 이용하여 대사활성계 적용(+S9 Mix) 및 비적용(-S9 Mix)하에서 유전 손상을 측정한 결과 모든 균주에서 대사활성계 적용 및 비적용 시 옥수수수염 추출물(5,000, 1,666.67, 555.56, 185.19, $61.73{\mu}g/plate$)에서 복귀돌연변이 평균 집락수의 변화 및 농도 의존적인 증가는 관찰되지 않았다. 염색체 이상 실험에서 대사활성계 적용 및 비적용 6시간 처리군(최고농도 $1,250{\mu}g/mL$)과 대사활성계 비적용 24시간 처리군(최고농도 $250{\mu}g/mL$)에서 이상 중기상 발현 빈도의 증가 및 음성대조군에 비하여 통계적으로 유의성이 확인되지 않았다. 마우스 골수세포에서의 소핵실험에서는 모든 투여용량의 옥수수수염 추출물(0, 500, 1,000, 2,000 mg/kg)에서 다염성 적혈구 중 소핵다염성 적혈구의 출현 빈도 및 총 적혈구에 대한 다염성 적혈구의 출현 빈도가 음성대조군과 비교하여 유의한 차이가 없었으므로 2,000 mg/kg 옥수수수염 추출물의 섭취는 마우스 골수세포의 소핵 유도에 영향을 주지 않는 것으로 판단된다. 결론적으로 세균에서의 복귀돌연변이 실험, 염색체 이상 실험 및 생체 내에서의 마우스 골수세포에서의 소핵시험을 통하여 본 실험 조건에서 고메이신 함유 옥수수수염 추출물은 유전독성을 유발하지 않는 것을 확인하였다.

Keywords

References

  1. Hasanudin K, Hashim P, Mustafa S. 2012. Corn silk (Stigma maydis) in healthcare: a phytochemical and pharmacological review. Molecules 17: 9697-9715. https://doi.org/10.3390/molecules17089697
  2. Maksimovic Z, Dobric S, Kovacevic N, Milovanovic Z. 2004. Diuretic activity of Maydis stigma extract in rats. Pharmazie 59: 967-971.
  3. George GO, Idu FK. 2015. Corn silk aqueous extracts and intraocular pressure of systemic and non-systemic hypertensive subjects. Clin Exp Optom 98: 138-149. https://doi.org/10.1111/cxo.12240
  4. Maksimovic Z, Malencic D, Kovacevic N. 2005. Polyphenol contents and antioxidant activity of Maydis stigma extracts. Bioresour Technol 96: 873-877. https://doi.org/10.1016/j.biortech.2004.09.006
  5. Waiss AC Jr, Chan BG, Elliger CA, Wiseman BR, McMillian WW, Widstrom NW, Zuber MS, Keaster AJ. 1979. Maysin, a flavone glycoside from corn silks with antibiotic activity toward corn earworm. J Econ Entomol 72: 256-158. https://doi.org/10.1093/jee/72.2.256
  6. Choi DJ, Kim SL, Choi JW, Park YI. 2014. Neuroprotective effects of corn silk maysin via inhibition of H2O2-induced apoptotic cell death in SK-N-MC cells. Life Sci 109: 57-64. https://doi.org/10.1016/j.lfs.2014.05.020
  7. Lee J, Kim SL, Lee S, Chung MJ, Park YI. 2014. Immunostimulating activity of maysin isolated from corn silk in murine RAW 264.7 macrophages. BMB Rep 47: 382-387. https://doi.org/10.5483/BMBRep.2014.47.7.191
  8. Peng KZ, Zhang SY, Zhou HL. 2016. Toxicological evaluation of the flavonoid-rich extract from Maydis stigma: Subchronic toxicity and genotoxicity studies in mice. J Ethnopharmacol 192: 161-169. https://doi.org/10.1016/j.jep.2016.07.012
  9. Organization for Economic Cooperation and Development (OECD). 2014. OECD Guideline for Testing of Chemicals (Guideline 487): in vitro Mammalian cells micronucleus test. OECD Publishing, Paris, France. p 1-26.
  10. European Food Safety Authority (EFSA). 2011. Scientific opinion on genotoxicity testing strategies applicable to food and feed safety assessment. EFSA J 9: 2379.
  11. Ames BN, Mccann J, Yamasaki E. 1975. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. Mutat Res 31: 347-364. https://doi.org/10.1016/0165-1161(75)90046-1
  12. Ishidate M Jr, Miura KF, Sofuni T. 1988. Chromosome aberration assays in genetic toxicology testing in vitro. Mutat Res 404: 167-172.
  13. Organization for Economic Cooperation and Development (OECD). 2014. OECD Guideline for Testing of Chemicals (Guideline 474): Mammalian erythrocyte micronucleus test. OECD Publishing, Paris, France. p 1-21.
  14. Kim SL, Kim MJ, Lee YY, Jung GH, Son BY, Lee JS, Kwon YU, Park YI. 2014. Isolation and identification of flavonoids from corn silk. Korean J Crop Sci 59: 435-444. https://doi.org/10.7740/kjcs.2014.59.4.435
  15. Mortelmans K, Zeiger E. 2000. The Ames Salmonella/microsome mutagenicity assay. Mutat Res 455: 29-60. https://doi.org/10.1016/S0027-5107(00)00064-6
  16. Kim BS, Margolin BH. 1999. Statistical methods for the Ames Salmonella assay: a review. Mutat Res 436: 113-122. https://doi.org/10.1016/S1383-5742(98)00025-8
  17. Kim BS, Zhao B, Kim HJ, Cho M. 2000. The statistical analysis of the in vitro chromosome aberration assay using Chinese hamster ovary cells. Mutat Res 469: 243-252. https://doi.org/10.1016/S1383-5718(00)00086-3
  18. Kastenbaum MA, Bowman KO. 1970. Tables for determining the statistical significance of mutation frequencies. Mutat Res 9: 527-549. https://doi.org/10.1016/0027-5107(70)90038-2
  19. Hayashi M, Yoshimura I, Sofuni T, Ishidate M Jr. 1989. A procedure for data analysis of the rodent micronucleus test involving a historical control. Environ Mol Mutagen 13: 347-356. https://doi.org/10.1002/em.2850130412
  20. Sumner DD, Cassidy JE, Szolics IM, Marco GJ, Bakshi KS, Brusick DJ. 1984. Evaluation of the mutagenic potential of corn (Zea mays L.) grown in untreated and atrazine (AAtrex(R)) treated soil in the field. Drug Chem Toxicol 7: 243-257. https://doi.org/10.3109/01480548409035106
  21. Peng KZ, Yang X, Zhou HL, Pan SX. 2015. Safety evaluation, in vitro and in vivo antioxidant activity of the flavonoid-rich extract from Maydis stigma. Molecules 20: 22102-22112. https://doi.org/10.3390/molecules201219835

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