Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Anti-Visceral Obesity Effect of Apios americana Medikus in Diet-Induced Obese Mice

식이로 유도한 비만마우스에서 아피오스의 내장지방 감소 효과

  • Choi, Ra-Yeong (Department of Food & Nutrition, Sunchon National University) ;
  • Lee, Jin (Department of Food & Nutrition, Sunchon National University) ;
  • Ryu, Hyo-Seon (Department of Food & Nutrition, Sunchon National University) ;
  • Ham, Ju Ri (Department of Food & Nutrition, Sunchon National University) ;
  • Park, Seok-Kyu (Department of Food & Nutrition, Sunchon National University) ;
  • Kim, Myung-Joo (Department of Bakery & Barista, Suseong College) ;
  • Lee, Mi-Kyung (Department of Food & Nutrition, Sunchon National University)
  • 최라영 (순천대학교 식품과학부 식품영양학전공) ;
  • 이진 (순천대학교 식품과학부 식품영양학전공) ;
  • 류효선 (순천대학교 식품과학부 식품영양학전공) ;
  • 함주리 (순천대학교 식품과학부 식품영양학전공) ;
  • 박석규 (순천대학교 식품과학부 식품영양학전공) ;
  • 김명주 (수성대학교 제과제빵커피과) ;
  • 이미경 (순천대학교 식품과학부 식품영양학전공)
  • Received : 2017.07.25
  • Accepted : 2017.08.11
  • Published : 2017.09.30
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Abstract

This study examined the effects of apios (Apios americana Medikus) supplementation on visceral obesity in high-fat diet-induced obese mice. C57BL/6N mice were fed a high-fat diet (40% calories from fat) with or without apios powder (10%, w/w) for 12 weeks. At the end of the experiment, apios supplementation reduced visceral fat mass significantly by 14.3% compared to the control group. Apios decreased significantly the atherogenic index, serum leptin level, hepatic lipid (free fatty acid and triglyceride) content, and lipid droplets, whereas it increased the serum high density lipoprotein-cholesterol/total cholesterol ratio. Hepatic lipogenic gene expression of peroxisome proliferator activated receptor gamma, fatty acid synthase, and diacylglycerol O-acyltransferase 2 was down-regulated by apios supplementation. These results suggest that apios is a healthy food for preventing high-fat diet-induced visceral obesity and fatty liver.

본 연구는 고지방식이(열량의 40%를 지방으로 공급)로 유도한 비만마우스에게 아피오스 분말(10%, w/w)을 12주간 급여한 후 내장지방 감소 및 지방간 개선 효과를 살펴보았다. 아피오스 분말 급여로 인한 유의적인 체중 감소는 보이지 않았으나, 부고환지방과 후복막지방 무게가 유의적으로 감소하여 총 내장지방 무게는 대조군에 비해 14.3% 감소하였다. 부고환지방조직의 지방세포 크기 역시 아피오스 분말군에서 현저히 감소되었다. 혈청의 유리지방산, 중성지질과 총 콜레스테롤 함량은 실험군 간의 유의적인 차이가 없었으나 총 콜레스테롤에 대한 HDL-콜레스테롤 비와 동맥경화 지수는 아피오스 분말군이 대조군에 비해 각각 18.9%와 47.2% 유의적으로 개선되었다. 혈청의 렙틴 함량은 아피오스 분말군이 대조군에 비해 유의적으로 낮았다. 고지방식이를 급여한 마우스에 아피오스 분말 급여는 간 조직 중의 지질축적 및 유리지방산과 중성지질 함량을 유의적으로 낮추는 것으로 나타났다. 간 조직의 지질합성 관련 유전자인 Pparg, Fasn과 Dgat2의 발현이 아피오스 분말 급여로 억제되었다. 이상의 결과에서 아피오스 분말은 고지방식이로 인한 내장지방 축적과 지방간을 개선할 수 있을 것으로 평가되었다.

Keywords

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