The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Suppression of colon cancer by administration of Canavalia gladiata D.C. and Arctium lappa L., Redix extracts in tumor-bearing mice model

종양이식 생쥐모델에서 도두(刀豆), 우방근(牛蒡根) 추출물의 대장암 억제 효과

  • Jang, Ji-Hye (School of Biological Sciences and Technology, Chonnam National University) ;
  • Ji, Kon-Young (School of Biological Sciences and Technology, Chonnam National University) ;
  • Choi, Hyung-Seok (Institute of Traditional Medicine and Bioscience, Daejeon University) ;
  • Yang, Won-Kyung (Institute of Traditional Medicine and Bioscience, Daejeon University) ;
  • Kim, Han-Young (Central Research Laboratories of Aekyung industrial Co.Ltd.) ;
  • Kim, Kun-hoae (Central Research Laboratories of Aekyung industrial Co.Ltd.) ;
  • Kang, Hyung-Sik (School of Biological Sciences and Technology, Chonnam National University) ;
  • Lee, Young-Cheol (Department of Herbology, College of Korean Medicine, Sangji University) ;
  • Kim, Seung-Hyung (Institute of Traditional Medicine and Bioscience, Daejeon University)
  • 장지혜 (전남대학교 생물과학.생명기술학과) ;
  • 지건영 (전남대학교 생물과학.생명기술학과) ;
  • 최형석 (대전대학교 동서생명과학연구원) ;
  • 양원경 (대전대학교 동서생명과학연구원) ;
  • 김한영 (애경중앙연구소) ;
  • 김근회 (애경중앙연구소) ;
  • 강형식 (전남대학교 생물과학.생명기술학과) ;
  • 이영철 (상지대학교 한의과대학) ;
  • 김승형 (대전대학교 동서생명과학연구원)
  • Received : 2017.08.17
  • Accepted : 2017.09.15
  • Published : 2017.09.30
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Abstract

Objective : In the present study, we examined whether Canavalia gladiata D.C. (CG) and Arctium lappa L., Redix (AL) mixture (CGAL), their components, lupeol and chicoric acid, regulate immune system and suppress the tumor in vitro and in vivo. Methods : LPS-induced reactive oxygen species (ROS) and nitric oxide (NO) were measured after treatment with CG extract (CGE), CGAL, lupeol, chicoric acid and lupeol and chicoric acid mixture (lupeol+CA) in Raw264.7 cell. To determine the effect of CGE on immune responses, immune cell population and IgG production were assessed in mice. To investigate the effect of CGAL and their component on anti-tumor activity, tumor volume and weight were measured, cell cycles and immune cell population were analyzed in MC38 injected tumor bearing mice. Also, NK cell activity was determined in splenocyte isolated from tumor bearing mice. Results : CGE, CGAL, lupeol, chicoric acid and lupeol+CA decreased the LPS-induced ROS and NO production without cell toxicity in RAW264.7 cells. CGE increased the immune cell populations of $CD4^+T$, $CD8^+T$ and macrophages in various immune organ of mice. In tumor bearing mice, CGAL, lupeol, chicoric acid and lupeol+CA suppressed tumor volume and weight. In cell cycle analysis, they decreased the percentages of S phase. In addition, CGAL, lupeol, chicoric acid and lupeol+CA immune cell populations of $CD4^+T$, $CD8^+Tcell$, NK cell and macrophage in tumor as well as NK cell activity. Conclusion : CGAL and its compounds may enhance immune responses and suppress tumor growth, and may be capable of developing health functional foods.

Keywords

References

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