Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antibacterial, Anti-Diarrhoeal, Analgesic, Cytotoxic Activities, and GC-MS Profiling of Sonneratia apetala (Buch.-Ham.) Seed

  • Received : 2017.05.18
  • Accepted : 2017.08.02
  • Published : 2017.09.30
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Abstract

Fruits of Sonneratia apetala (Buch.-Ham.), (English: mangrove apple, Bengali: keora) both seeds and pericarps, are largely consumed as food besides their enormous medicinal application. The fruit seeds have high content of nutrients and bioactive components. The seeds powder of S. apetala was successively fractionated using n-hexane, diethyl ether, chloroform, ethyl acetate, and methanol. The fractions were used to evaluate antibacterial, anti-diarrhoeal, analgesic, and cytotoxic activities. Methanol fraction of seeds (MeS) stronly inhibited Escherichia coli strains, Salmonella Paratyphi A, Salmonella Typhi, Shigella dysenteriae, and Staphylococcus aureus except Vibrio cholerae at $500{\mu}g/disc$. All the fractions strongly inhibited castor oil induced diarrhoeal episodes and onset time in mice at 500 mg extract/kg body weight (P<0.001). At the same concentration, MeS had the strongest inhibitory activity on diarrhoeal episodes, whereas the n-hexane fraction (HS) significantly (P<0.05) prolonged diarrhoeal onset time as compared to positive control. Similarly, HS (P<0.005) inhibited acetic acid induced writhing in mice at 500 mg extract/kg, more than any other fraction. HS and diethyl ether fractions of seed strongly increased reaction time of mice in hot plate test at 500 mg extract/kg. All the fractions showed strong cytotoxic effects in brine shrimp lethality tests. Gas chromatography-mass spectrometry analysis of HS led to the identification of 23 compounds. Linoleic acid (29.9%), palmitic acid (23.2%), ascorbyl palmitate (21.2%), and stearic acid (10.5%) were the major compounds in HS. These results suggest that seeds of S. apetala could be of great use as nutraceuticals.

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References

  1. Das S, Siddiqi NA. 1985. The mangroves and mangrove forests of Bangladesh. Mangrove silviculture division, bulletin no. 2. Bangladesh Forest Research Institute, Chittagonge, Bangladesh.
  2. Tomlinson PB. 1994. The botany of mangroves. Cambridge University Press, Cambridge, UK.
  3. Das S, Ghose M. 2003. Seed structure and germination pattern of some Indian mangroves with taxonomic relevance. Taiwania 48: 287-298.
  4. Hossain SJ, Basar MH, Rokeya B, Arif KMT, Sultana MS, Rahman MH. 2013. Evaluation of antioxidant, antidiabetic and antibacterial activities of the fruit of Sonneratia apetala (Buch.-Ham.). Orient Pharm Exp Med 13: 95-102. https://doi.org/10.1007/s13596-012-0064-4
  5. Bandaranayake WM. 1998. Traditional and medicinal uses of mangroves. Mangroves and Salt Marshes 2: 133-148. https://doi.org/10.1023/A:1009988607044
  6. Teja VP, Ravishankar K. 2013. Preliminary phytochemical investigation and in vitro antimicrobial activity of ethanol extract of Sonneratia apetala. Int Res J Pharm 4: 84-87.
  7. Patra JK, Das SK, Thatoi H. 2015. Phytochemical profiling and bioactivity of a mangrove plant, Sonneratia apetala, from Odisha Coast of India. Chin J Integr Med 21: 274-285. https://doi.org/10.1007/s11655-014-1854-y
  8. Shefa AA, Baishakhi FS, Islam S, Sadhu SK. 2014. Phytochemical and pharmacological evaluation of fruits of Sonneratia apetala. GJMR-B-Pharma, Drug Discovery, Toxicology and Medicine 14: 1-6.
  9. Hossain SJ, Iftekharuzzaman M, Haque MA, Saha B, Moniruzzaman M, Rahman MM, Hossain H. 2016. Nutrient compositions, antioxidant activity, and common phenolics of Sonneratia apetala (Buch.-Ham.) fruit. Int J Food Prop 19: 1080-1092. https://doi.org/10.1080/10942912.2015.1055361
  10. Hossain SJ, Pervin T, Suma SA. 2016. Effects of cooking methods at different time durations on total phenolics and antioxidant activities of fresh and dried-stored fruits of Sonneratia apetala (Buch.-Ham.). Int Food Res J 23: 556-563.
  11. Shoba FG, Thomas M. 2001. Study of antidiarrhoeal activity of four medicinal plants in castor-oil induced diarrhoea. J Ethnopharmacol 76: 73-76. https://doi.org/10.1016/S0378-8741(00)00379-2
  12. Koster R, Anderson M, De Beer EJ. 1959. Acetic acid-induced analgesic screening. Fed Proc 18: 412.
  13. Eddy NB, Leimbach D. 1953. Synthetic analgesics. II. Dithienylbutenyl-and dithienylbutylamines. J Pharmacol Exp Ther 107: 385-393.
  14. McLaughlin JL. 1991. Crown gall tumors on potato disc and brine shrimp lethality: two simple assays for higher plant screening and fractionation. In Methods in Plant Biochemistry Assays for Bioactivity. Dey PM, Harborne JB, Hostettman K, eds. Academic Press, San Diego, CA, USA. Vol 6, p 1-32.
  15. Bobbarala V, Vadlapudi V, Naidu KC. 2009. Mangrove plant Sonneratia apetala antimicrobial activity on selected pathogenic microorganisms. Orient J Chem 25: 445-447.
  16. Arif KMT, Datta RK, Sarower MG, Hossain SJ. 2014. Comparison of phenolics and antibacterial activity of commonly used antidiabetic medicinal plants in Bangladesh. PharmacologyOnline 1: 7-15.
  17. Cowan MM. 1999. Plant products as antimicrobial agents. Clin Microbiol Rev 12: 564-582.
  18. Pierce NF, Carpenter CC Jr, Elliott HL, Greenough WB 3rd. 1971. Effects of prostaglandins, theophylline, and cholera exotoxin upon transmucosal water and electrolyte movement in the canine jejunum. Gastroenterology 60: 22-32.
  19. Saito T, Miyake M, Toba M, Okamatsu H, Shimizu S, Noda M. 2002. Inhibition by apple polyphenols of ADP-ribosyltransferase activity of cholera toxin and toxin-induced fluid accumulation in mice. Microbiol Immunol 46: 249-255. https://doi.org/10.1111/j.1348-0421.2002.tb02693.x
  20. Collier HO, Dinneen LC, Johnson CA, Schneider C. 1968. The abdominal constriction response and its suppression by analgesic drugs in the mouse. Br J Pharmacol Chemother 32: 295-310. https://doi.org/10.1111/j.1476-5381.1968.tb00973.x
  21. Whittle BA. 1964. The use of changes in capillary permeability in mice to distinguish between narcotic and nonnarcotic analgesics. Br J Pharmacol Chemother 22: 246-253. https://doi.org/10.1111/j.1476-5381.1964.tb02030.x
  22. Yaksh TL, Rudy TA. 1977. Studies on the direct spinal action of narcotics in the production of analgesia in the rat. J Pharmacol Exp Ther 202: 411-428.
  23. Jimenez-Aspee F, Alberto MR, Quispe C, Soriano Mdel P, Theoduloz C, Zampini IC, Isla MI, Schmeda-Hirschmann G. 2015. Anti-inflammatory activity of copao (Eulychnia acida Phil., Cactaceae) fruits. Plant Foods Hum Nutr 70: 135-140. https://doi.org/10.1007/s11130-015-0468-7
  24. Hossain SJ, Tsujiyama I, Takasugi M, Islam MA, Biswas RS, Aoshima H. 2008. Total phenolic content, antioxidative, anti-amylase, anti-glucosidase, and antihistamine release activities of Bangladeshi fruits. Food Sci Technol Res 14: 261-268. https://doi.org/10.3136/fstr.14.261
  25. Tsujiyama I, Mubassara S, Aoshima H, Hossain SJ. 2013. Anti-histamine release and anti-inflammatory activities of aqueous extracts of citrus fruits peels. Orient Pharm Exp Med 13: 175-180. https://doi.org/10.1007/s13596-012-0093-z
  26. Logarto Parra A, Silva Yhebra R, Guerra Sardinas I, Iglesias Buela L. 2001. Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose ($LD_{50}$ value) in mice, to determine oral acute toxicity of plant extracts. Phytomedicine 8: 395-400. https://doi.org/10.1078/0944-7113-00044
  27. Rieser MJ, Gu ZM, Fang XP, Zeng L, Wood KV, McLaughlin JL. 1996. Five novel mono-tetrahydrofuran ring acetogenins from the seeds of Annona muricata. J Nat Prod 59: 100-108. https://doi.org/10.1021/np960037q
  28. Hossain SJ, Kato H, Aoshima H, Yokoyama T, Yamada M, Hara Y. 2002. Polyphenol-induced inhibition of the response of $Na^+$/glucose cotransporter expressed in Xenopus oocytes. J Agric Food Chem 50: 5215-5219. https://doi.org/10.1021/jf020252e
  29. Hossain SJ, Aoshima H, Koda H, Kiso Y. 2007. Review of functional studies of beverage components acting on the recombinant GABAA neuroreceptor, and $Na^+$/glucose cotransporter-response using the Xenopus oocyte expression system and electrophysiological measurements. Food Biotechnol 21: 237-270. https://doi.org/10.1080/08905430701534081
  30. Saeed NM, El-Demerdash E, Abdel-Rahman HM, Algandaby MM, Al-Abbasi FA, Abdel-Naim AB. 2012. Anti-inflammatory activity of methyl palmitate and ethyl palmitate in different experimental rat models. Toxicol Appl Pharmacol 264: 84-93. https://doi.org/10.1016/j.taap.2012.07.020
  31. Hunter JE, Zhang J, Kris-Etherton PM. 2010. Cardiovascular disease risk of dietary stearic acid compared with trans, other saturated, and unsaturated fatty acids: a systematic review. Am J Clin Nutr 91: 46-63. https://doi.org/10.3945/ajcn.2009.27661
  32. Rinelli S, Spadafranca A, Fiorillo G, Cocucci M, Bertoli S, Battezzati A. 2012. Circulating salicylic acid and metabolic and inflammatory responses after fruit ingestion. Plant Foods Hum Nutr 67: 100-104. https://doi.org/10.1007/s11130-012-0282-4