Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지
DOI QR코드

DOI QR Code

Study on Ovum-pick up for Improvement of Embryo Transfer Efficiency in Hanwoo Cows

한우의 수정란이식 효율성 향상을 위한 생체난포란 채취에 관한 연구

  • 조상래 (농촌진흥청 국립축산과학원) ;
  • 강성식 (농촌진흥청 국립축산과학원) ;
  • 김의형 (농촌진흥청 국립축산과학원) ;
  • 이석동 (농촌진흥청 국립축산과학원) ;
  • 이명숙 (농촌진흥청 국립축산과학원) ;
  • 양병철 (농촌진흥청 국립축산과학원)
  • Received : 2017.08.18
  • Accepted : 2017.09.15
  • Published : 2017.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Commercial applications of OPU/IVP were to produce embryos and calves from high genetic cows. The aim of this present study was to compare the number of recovered oocytes and cultured In vitro produced embryos from Ovum Pick-up (OPU). OPU derived embryo production was carried out of oocytes by ultrasonographic guided follicular aspiration and then produced in vitro produced blastocysts by IVP culture system. In result, the rate of recovered oocytes was obtained 612 (57.2%) and 451(73.7) G1+G2 grade oocytes. No difference of recovered rate (51.1~62.1%) was seen in six donor. The rate of cleavage and blastocyst development were obtained 320 (70.9%) and 78 (24.4%) that was $3.3{\pm}0.4$ cleaved embryo and $0.9{\pm}0.2$ blastocysts per session. Cleavage rate of OPU oocytes in No. 6 donor was 90.6%, significantly (P<0.05) higher than that in the other donors, However, blastocysts was similar (25.8~30.0%). In conclusion, limited numbers of OPU oocytes had competent development when cultured in SOF culture medium.

Keywords

References

  1. Abe H, Shiku H, Aoyagi S and Hoshi H. 2004. In vitro culture and evaluation of embryos for production of high quality bovine embryos. J. Mamm. Ova. Res. 21:22-30. https://doi.org/10.1274/jmor.21.22
  2. Broadbent PJ, Dolman DF, Watt RG, Smith AK and Franklin MF. 1997. Effect of frequency of follicle aspiration on oocyte yield and subsequent superovulatory response in cattle. Theriogenology 47:1027-1040. https://doi.org/10.1016/S0093-691X(97)00060-5
  3. Chaubal SA, Ferre LB, Molina JA, Faber DC, Bols PJ, Rezamand PTX and Yang X. 2007. Hormonal treatments for increasing the oocyte and embryo production in an OPU-IVP system. Thriogenology 67:719-728 https://doi.org/10.1016/j.theriogenology.2006.07.022
  4. Galli C, Crotti G, Notari C, Turini P, Duchi R and Lazzari G. 2000. Embryo production by ovum pick-up from live donors. Thriogenology 55:1341-1357.
  5. Hasler JF, McCauley AD, Lathrop WF and Foote RH. 1987. Effect of donor-embryo-recipient interactions on pregnancy rate in a large-scale bovine embryo transfer program. Theriogenology 27:139-168. https://doi.org/10.1016/0093-691X(87)90075-6
  6. Hasler JF. 1998. The current status of oocytes recovery, in vitro embryo production, and embryo transfer in domestic animals, with an emphasis on bovine. J. Anim. Sci. 76(Suppl.3):52-74. https://doi.org/10.2527/1998.76suppl_352x
  7. Holm P and Callesen H. 1998. In vivo versus in vitro produced bovine ova: similarities and differences relevant for practical application. Reprod. Dev. Nutr. 38:579-594. https://doi.org/10.1051/rnd:19980601
  8. Looney CR, Lindsey BR, Gonseth CL and Johnson DL. 1994. Commercial aspects of oocytes retrival and in vitro fertilization (IVF) for embryo production in problem cows. Thriogenology 41:67-72. https://doi.org/10.1016/S0093-691X(05)80050-0
  9. Lopez AS, Larsen LH, Ramsing N, Lovendahl P, Raty M, Peippo J, Greve T and Callesen H. 2005. Respiration rates of individual bovine in vitro-produced embryos measured with a novel, non-invasive and highly sensitive microsensor system. Reproduction 130:669-679. https://doi.org/10.1530/rep.1.00703
  10. Pieterse MC, Kappen KA, Kruip TA and Taverne MA. 1988. Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries. Theriogenology 30:751-762. https://doi.org/10.1016/0093-691X(88)90310-X
  11. Sakagami N, Yamamoto T, Akiyama K, Nakazawa Y, Kojima N, Nishida K, Yokomizo S, Takagi Y, Abe H, Suzuki C and Yoshioka K. 2010. Viability of porcine embryos after vitrification using water-soluble pullulan films. J. Reprod. Dev. 56:279-284. https://doi.org/10.1262/jrd.09-101H
  12. Scheider HJ, Castleberry RS and Griffin JL. 1980. Commercial aspects of bovine embryo transfer. Theriogenology 13:73-85. https://doi.org/10.1016/0093-691X(80)90016-3
  13. Scott CA, Robertson L, de Moura R.T.D, Paterson C and Boyd JS. 1994. Technical aspects of transvaginal ultrasound-guided follicular aspiration in cows. Vet. Record, 134:440-443. https://doi.org/10.1136/vr.134.17.440
  14. Sreenan JM and Diskin MG. 1989. Effect of a unilateral or bilateral twin embryo distribution on twinning and embryo survival rate in the cow. Biol. Reprod. 87:657-664.
  15. Ward FA, Lonergan P, Enright BP and Boland MP. 2000. Factors affecting recovery and quality of oocytes for bovine embryo production in vitro using ovum pick-up technology. Theriogenology 54:433-446. https://doi.org/10.1016/S0093-691X(00)00360-5