Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Study on antioxidant and anti-inflammatory activity of compound K extract produced by Saccharomyces servazzii(GB-07) strain derived from traditional soy

전통장류 유래 GB-07균주에 의해 생산된 Compound K 함유 발효인삼의 항산화 및 항염증 활성 연구

  • 신동규 (제너럴바이오(주)) ;
  • 서정훈 (제너럴바이오(주)) ;
  • 조상민 (제너럴바이오(주)) ;
  • 최학주 (대전대학교 난치성면역질환의동서생명의학연구센터)
  • Received : 2017.05.11
  • Accepted : 2017.09.15
  • Published : 2017.09.30
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Abstract

Ginseng, whichhas long been used for its medicinal properties, has recently been investigated by scientific research to identify its components and evaluate its efficacy. Recently, two components of ginseng, Rg3 and compound K, have been attracting attention and various functional materials containing these materials have been developed and investigated. In this study, compound K wasproduced using yeast (Saccharomyces servazzii, GB-07) and To be used for industrialization. The antioxidant and anti-inflammatory activity of compound K (containing 20 g/g) ginseng fermented extract was investigated. In the fermented ginseng extract, the free radical DPPH was scavenged in a concentration-dependent manner and the production of ROS was inhibited in macrophages(RAW 264.7 cell). Moreover, the LPS-induced inflammatory cytokines $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ were suppressed. These results suggest the possibility of industrialization viathe development of products containing compound K through future process development and various efficacy tests.

인삼은 약용으로 수 천년 동안 소중하게 사용되어 왔으며 근대에 들어와 단일 성분의 분리와 각 성분에 대한 효능의 과학적인 연구가 활발히 이어져 오고 있다. 최근에는 인삼 성분 중에 Rg3, compound K 등이 주목 받고 있으며 기능성을 갖는 다양한 소재들이 연구 개발되어 제품화 되고 있다. 본 연구에서는 전통 장류에서 유래한 효모균 (Saccharomyces servazzii, GB-07)을 이용하여 인삼 추출물을 발효하여 compound K를 생산하고 기능성을 확인하여 상업적 이용 가능성을 확인하였다. Compound K 함유 ($20{\mu}g/g$ 함유) 인삼 발효 추출물을 이용하여 항산화 및 항염증 활성을 측정하였다. 발효 인삼추출물은 자유 라디칼 DPPH (di(phenyl) -(2,4,6-trinitrophenyl)를 농도 의존적으로 소거하였으며. macrophage(RAW 264.7 cell)에서 ROS의 생성을 농도 의존적으로 억제하였다. 또한 LPS에 의해 유도된 염증 사이토카인 $IL-1{\beta}$와 IL-6 그리고 $TNF-{\alpha}$의 생성을 억제하는 것으로 나타났다. 본 연구 결과는 향후 공정개발과 다양한 효능 시험을 통해 compound K를 함유한 제품의 개발을 통한 산업화의 가능성 시사하고 있다.

Keywords

References

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