The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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A Novel Recombined Potato virus Y Isolate in China

  • Han, Shuxin (College of Agricultural, Northeast Agricultural University) ;
  • Gao, Yanling (College of Agricultural, Northeast Agricultural University) ;
  • Fan, Guoquan (Virus-free Seeding Research Institute of Heilongjiang Academy of Agricultural Sciences) ;
  • Zhang, Wei (College of Agricultural, Northeast Agricultural University) ;
  • Qiu, Cailing (Virus-free Seeding Research Institute of Heilongjiang Academy of Agricultural Sciences) ;
  • Zhang, Shu (Virus-free Seeding Research Institute of Heilongjiang Academy of Agricultural Sciences) ;
  • Bai, Yanju (Virus-free Seeding Research Institute of Heilongjiang Academy of Agricultural Sciences) ;
  • Zhang, Junhua (College of Agricultural, Northeast Agricultural University) ;
  • Spetz, Carl (Norwegian Institute of Bieconomy Research)
  • Received : 2016.09.13
  • Accepted : 2017.05.20
  • Published : 2017.08.01
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Abstract

This study reports the findings of a distinct Potato virus Y (PVY) isolate found in Northeast China. One hundred and ten samples (leaves and tubers) were collected from potato plants showing mosaic symptoms around the city of Harbin in Heilongjiang province of China. The collected tubers were planted and let to grow in a greenhouse. New potato plants generated from these tubers showed similar symptoms, except for one plant. Subsequent serological analyses revealed PVY as the causing agent of the disease. A novel PVY isolate (referred to as HLJ-C-44 in this study) was isolated from this sample showing unique mild mosaic and crisped leaf margin symptoms. The complete genome of this isolate was analyzed and determined. The results showed that HLJ-C-44 is a typical PVY isolate. Phylogenetic analysis indicated that this isolate belongs to the N-Wi strain group of PVY recombinants ($PVY^{N-Wi}$) and also shared the highest overall sequence identity (nucleotide and amino acid) with other members of this strain group. However, recombination analysis of isolate HLJ-C-44 revealed a recombination pattern that differed from that of other $PVY^{N-Wi}$ isolates. Moreover, biological assays in four different potato cultivars and in Nicotiana tabacum also revealed a different phenotypic response than that of a typical $PVY^{N-Wi}$ isolate. This data, combined, suggest that HLJ-C-44 is a novel PVY recombinant with distinct biological properties.

Keywords

References

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