Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Ginsenoside Rg1 from Panax ginseng enhances myoblast differentiation and myotube growth

  • Go, Ga-Yeon (Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University) ;
  • Lee, Sang-Jin (Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University) ;
  • Jo, Ayoung (Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University) ;
  • Lee, Jaecheol (Division of Cardiology, Department of Medicine, Stanford University School of Medicine) ;
  • Seo, Dong-Wan (College of Pharmacy, Dankook University) ;
  • Kang, Jong-Sun (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute) ;
  • Kim, Si-Kwan (Department of Biomedical Chemistry, College of Biomedical & Health Science, Konkuk University) ;
  • Kim, Su-Nam (Natural Products Research Institute, Korea Institute of Science and Technology) ;
  • Kim, Yong Kee (Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University) ;
  • Bae, Gyu-Un (Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University)
  • Received : 2017.05.04
  • Accepted : 2017.05.25
  • Published : 2017.10.15
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Abstract

Background: Ginsenoside Rg1 belongs to protopanaxatriol-type ginsenosides and has diverse pharmacological activities. In this report, we investigated whether Rg1 could upregulate muscular stem cell differentiation and muscle growth. Methods: C2C12 myoblasts, MyoD-transfected 10T1/2 embryonic fibroblasts, and HEK293T cells were treated with Rg1 and differentiated for 2 d, subjected to immunoblotting, immunocytochemistry, or immunoprecipitation. Results: Rg1 activated promyogenic kinases, p38MAPK (mitogen-activated protein kinase) and Akt signaling, that in turn promote the heterodimerization with MyoD and E proteins, resulting in enhancing myogenic differentiation. Through the activation of Akt/mammalian target of rapamycin pathway, Rg1 induced myotube growth and prevented dexamethasone-induced myotube atrophy. Furthermore, Rg1 increased MyoD-dependent myogenic conversion of fibroblast. Conclusion: Rg1 upregulates promyogenic kinases, especially Akt, resulting in improvement of myoblast differentiation and myotube growth.

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References

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