Allergy, Asthma & Immunology Research

The Korean Academy of Asthma, Allergy and Clinical Immunology (대한천식알레르기학회)
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Monoclonal Antibodies to Recombinant Fag e 3 Buckwheat Allergen and Development of a Two-site ELISA for Its Quantification

  • Jeong, Kyoung Yong (Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine) ;
  • Park, Kyung Hee (Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine) ;
  • Lee, Jae-Hyun (Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine) ;
  • Park, Jung-Won (Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine)
  • Received : 2016.10.14
  • Accepted : 2017.03.14
  • Published : 2017.09.01
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Abstract

Purpose: Buckwheat is a major cause of anaphylaxis, and Fag e 3 is the key major allergen in buckwheat. However, an immunoassay system for the quantification of Fag e 3 has yet to be developed. Methods: We developed a 2-site enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies (mAbs) produced against recombinant Fag e 3. We applied this ELISA to quantify native Fag e 3 in total buckwheat extract. Results: Four clones of mAbs were produced, and all recognized vicilin allergens not only from buckwheat, but also from peanut and walnut. However, the ELISA using these antibodies was only able to quantify Fag e 3 in the total extract after addition of 1% sodium dodecyl sulphate (SDS) and heating, which facilitated dissociation of the allergen. The detection limit of the developed 2-site ELISA was $0.8{\mu}g/mL$. The measurement of Fag e 3 in the total extract of buckwheat showed that approximately 12% of protein in total buckwheat extract was Fag e 3. Conclusions: We have developed an ELISA system for the quantification of the group 3 buckwheat allergen, Fag e 3, specifically. This assay will be useful for standardization of buckwheat allergens and monitoring of buckwheat contamination in foods.

Keywords

Acknowledgement

Supported by : Korea Centers for Disease Control & Prevention, Korean Health Industry Development Institute (KHIDI)

References

  1. Han SM, Heo YR. Changes of prevalence of food allergy in elementary school student and perception of it in school nutritionist in Korea, 1995-2015. J Nutr Health 2016;49:8-17. https://doi.org/10.4163/jnh.2016.49.1.8
  2. Kim SR, Park HJ, Park KH, Lee JH, Park JW. IgE sensitization patterns to commonly consumed foods determined by skin prick test in Korean adults. J Korean Med Sci 2016;31:1197-201. https://doi.org/10.3346/jkms.2016.31.8.1197
  3. Lee SY, Ahn K, Kim J, Jang GC, Min TK, Yang HJ, et al. A multicenter retrospective case study of anaphylaxis triggers by age in Korean children. Allergy Asthma Immunol Res 2016;8:535-40. https://doi.org/10.4168/aair.2016.8.6.535
  4. Chandrupatla CV, Kundu RV, Aronson IK. Buckwheat allergy and atopic dermatitis. J Am Acad Dermatol 2005;53:356-7. https://doi.org/10.1016/j.jaad.2005.02.044
  5. Heffler E, Nebiolo F, Asero R, Guida G, Badiu I, Pizzimenti S, et al. Clinical manifestations, co-sensitizations, and immunoblotting profiles of buckwheat-allergic patients. Allergy 2011;66:264-70. https://doi.org/10.1111/j.1398-9995.2010.02469.x
  6. Taylor SL, Baumert JL. Worldwide food allergy labeling and detection of allergens in processed foods. Chem Immunol Allergy 2015; 101:227-34.
  7. Park JW, Kang DB, Kim CW, Ko SH, Yum HY, Kim KE, et al. Identification and characterization of the major allergens of buckwheat. Allergy 2000;55:1035-41. https://doi.org/10.1034/j.1398-9995.2000.00763.x
  8. Choi SY, Sohn JH, Lee YW, Lee EK, Hong CS, Park JW. Characterization of buckwheat 19-kD allergen and its application for diagnosing clinical reactivity. Int Arch Allergy Immunol 2007;144:267-74. https://doi.org/10.1159/000106315
  9. Maruyama N, Sato S, Yanagida N, Cabanos C, Ito K, Borres MP, et al. Clinical utility of recombinant allergen components in diagnosing buckwheat allergy. J Allergy Clin Immunol Pract 2016;4:322-323.e3. https://doi.org/10.1016/j.jaip.2015.11.028
  10. Koeberl M, Clarke D, Lopata AL. Next generation of food allergen quantification using mass spectrometric systems. J Proteome Res 2014;13:3499-509. https://doi.org/10.1021/pr500247r
  11. Jeong KY, Hong CS, Lee JS, Park JW. Optimization of allergen standardization. Yonsei Med J 2011;52:393-400. https://doi.org/10.3349/ymj.2011.52.3.393
  12. Shewry PR, Napier JA, Tatham AS. Seed storage proteins: structures and biosynthesis. Plant Cell 1995;7:945-56. https://doi.org/10.1105/tpc.7.7.945
  13. Villalta D, Conte M, Asero R, Da Re M, Stella S, Martelli P. Isolated IgE reactivity to native walnut vicilin-like protein (nJug r 2) on ISACTM microarray is due to cross-reactive carbohydrate epitopes. Clin Chem Lab Med 2013;51:1991-5.
  14. Barre A, Sordet C, Culerrier R, Rancc F, Didier A, Rougc P. Vicilin allergens of peanut and tree nuts (walnut, hazelnut and cashew nut) share structurally related IgE-binding epitopes. Mol Immunol 2008;45:1231-40. https://doi.org/10.1016/j.molimm.2007.09.014
  15. Badenoch-Jones J, Spencer D, Higgins TJ, Millerd A. The role of glycosylation in storage-protein synthesis in developing pea seeds. Planta 1981;153:201-9. https://doi.org/10.1007/BF00383888
  16. Yang ZH, Li C, Li YY, Wang ZH. Effects of Maillard reaction on allergenicity of buckwheat allergen Fag t 3 during thermal processing. J Sci Food Agric 2013;93:1510-5. https://doi.org/10.1002/jsfa.5928
  17. Pomcs A, Vinton R, Chapman MD. Peanut allergen (Ara h 1) detection in foods containing chocolate. J Food Prot 2004;67:793-8. https://doi.org/10.4315/0362-028X-67.4.793
  18. Shin DS, Compadre CM, Maleki SJ, Kopper RA, Sampson H, Huang SK, et al. Biochemical and structural analysis of the IgE binding sites on Ara h 1, an abundant and highly allergenic peanut protein. J Biol Chem 1998;273:13753-9. https://doi.org/10.1074/jbc.273.22.13753
  19. de Jong EC, Van Zijverden M, Spanhaak S, Koppelman SJ, Pellegrom H, Penninks AH. Identification and partial characterization of multiple major allergens in peanut proteins. Clin Exp Allergy 1998;28:743-51. https://doi.org/10.1046/j.1365-2222.1998.00301.x
  20. Pomcs A, Helm RM, Bannon GA, Burks AW, Tsay A, Chapman MD. Monitoring peanut allergen in food products by measuring Ara h 1. J Allergy Clin Immunol 2003;111:640-5. https://doi.org/10.1067/mai.2003.118
  21. Perry TT, Conover-Walker MK, Pomcs A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol 2004;113:973-6. https://doi.org/10.1016/j.jaci.2004.02.035
  22. Lee SY, Lee KS, Hong CH, Lee KY. Three cases of childhood nocturnal asthma due to buckwheat allergy. Allergy 2001;56:763-6. https://doi.org/10.1034/j.1398-9995.2001.056008763.x
  23. Park HS, Nahm DH. Buckwheat flour hypersensitivity: an occupational asthma in a noodle maker. Clin Exp Allergy 1996;26:423-7. https://doi.org/10.1111/j.1365-2222.1996.tb00558.x
  24. Wensing M, Knulst AC, Piersma S, O'Kane F, Knol EF, Koppelman SJ. Patients with anaphylaxis to pea can have peanut allergy caused by cross-reactive IgE to vicilin (Ara h 1). J Allergy Clin Immunol 2003;111:420-4. https://doi.org/10.1067/mai.2003.61
  25. Matsuda R, Yoshioka Y, Akiyama H, Aburatani K, Watanabe Y, Matsumoto T, et al. Interlaboratory evaluation of two enzyme-linked immunosorbent assay kits for the detection of egg, milk, wheat, buckwheat, and peanut in foods. J AOAC Int 2006;89:1600-8.
  26. Choi SY, Sohn JH, Lee YW, Lee EK, Hong CS, Park JW. Application of the 16-kDa buckwheat 2 S storage albumin protein for diagnosis of clinical reactivity. Ann Allergy Asthma Immunol 2007;99:254-60. https://doi.org/10.1016/S1081-1206(10)60661-8
  27. Lee SE, Kim H. Update on early nutrition and food allergy in children. Yonsei Med J 2016;57:542-8. https://doi.org/10.3349/ymj.2016.57.3.542

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