Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Methoxy PEG-45 Thioctate (LA-PEG) against Oxidative Protein Damage and Anti-glycation

Methoxy PEG-45 Thioctate (LA-PEG)의 항노화 효과에 대한 연구

  • 김진화 ((주)잇츠한불 기술연구원) ;
  • 오정영 ((주)잇츠한불 기술연구원) ;
  • 배준태 ((주)잇츠한불 기술연구원) ;
  • 이근수 ((주)잇츠한불 기술연구원) ;
  • 표형배 ((주)잇츠한불 기술연구원)
  • Received : 2017.09.16
  • Accepted : 2017.09.26
  • Published : 2017.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Aging is a physiological and irreversible, progressive process involving changes in the ability to maintain cellular functionality. It affects tissues, organs and the whole organism and thus finally cause to death. Oxidative stress has been postulated to contribute significantly to the accelerated accumulation of advanced glycation endproducts (AGEs) in collagen, which is implicated in the process of skin aging. In the present study, glycation inhibitory activity of methoxy PEG-45 thioctate (LA-PEG), and its inhibitory effect of cellular oxidation and senescence was investigated. Treatment of LA-PEG significantly showed lower fluorescent intensity induced by AGEs. In addition, LA-PEG was significantly reduced the formation of ROS induced by AGEs. High antioxidant and anti-glycation activities of LA-PEG in glycated collagen model indicated its contribution to anti-aging process. Cellular senescence leads to an increase in senescence-associated ${\beta}$-galactosidase ($SA-{\beta}-gal$) activity, which can be used as a biomarker to identify senescent cells. Treatment with LA-PEG showed a dose-dependent, statistically significant decreased in $SA-{\beta}-gal$ indicating reduced senescence. These results suggest that LA-PEG may have potent anti-aging effects and can be used as new functional materials against cellular accumulation of AGEs.

노화는 생리학적으로 비가역적으로 일어나는 과정으로 노화가 진행됨에 따라 단백질의 산화화학반응 등으로 노화징후가 축적된다. 활성산소와 당화된 최종 당화산물(advanced glycation endproducts, AGEs 최종 당화산물)은 생체 조직과 세포를 공격하여 노화를 촉진한다고 알려져 있다. 본 연구에서는 항산화 물질로 알려진 aminoguanidine을 양성 대조군으로 anti-glycation 효과를 확인하였으며, methoxy PEG-45 thioctate(LA-PEG)를 농도별로 처리하여 anti-glycation 효과를 평가하였다. 실험결과 LA-PEG는 매우 우수한 anti-glycation 효과로 최종 당화산물(AGEs) 생성억제 활성이 매우 우수하게 나타났으며, 항산화 효과와 밀접한 연관을 나타냈다. 또, 세포노화 지표물질인 senescence-associated ${\beta}$-galactosidase ($SA-{\beta}-gal$) 활성을 사람 섬유아세포(HDF)를 이용하여 확인한 결과, LA-PEG를 처리하였을 때 염색된 세포의 수가 감소하여 세포의 senescence를 억제하는 것을 확인할 수 있었다. 본 연구결과, LA-PEG의 anti-glycation 효과 및 산화로 인한 단백질 손상에 대한 보호 효과가 우수하게 나타났으며, 항노화 화장품에 적용 시 효과적으로 적용할 수 있을 것으로 사료된다.

Keywords

References

  1. G. Jenkins, Molecular mechanisms of skin ageing, Mech. Ageing. Dev., 123, 801 (2002). https://doi.org/10.1016/S0047-6374(01)00425-0
  2. E. J. Middleton, C. Kandaswami, and T. C. Theoharides, The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer, Pharmacol. Rev., 52(4), 673 (2000).
  3. E. Naru, T. Suzuki, M. Moriyama, K. Inomata, A. Hayashi, K. Arakane, and K. Kaji, Functional changes induced by chronic UVA irradiation to cultured human dermal fibroblasts, Br. J. Dermatol., 153, 6 (2005).
  4. T. Jung, B. Catalgol, and T. Grune, The proteasomal system, Mol. Aspects Med., 30(4), 191 (2009). https://doi.org/10.1016/j.mam.2009.04.001
  5. K. Bedard and K. H. Krause, The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology, Physiol Rev., 87, 245 (2007). https://doi.org/10.1152/physrev.00044.2005
  6. K. Nowotny, T. Jung, T. Grune, and A. Hohn, Accumulation of modified proteins and aggregate formation in aging, Exp. Gerontol., 57, 122 (2014). https://doi.org/10.1016/j.exger.2014.05.016
  7. T. Kueper, T. Grune, S. Prahl, H. Lenz, V. Welge, T. Biernoth, Y. Vogt, G. M. Muhr, A. Gaemlich, T. Jung, G. Boemke, H. P. Elsasser, K. P. Wittern, H. Wenck, F. Stab, and T. Blatt, Vimentin is the specific target in skin glycation, structural prerequisites, functional consequences, and role in skin aging, J. Biol. Chem., 282, 23427 (2007). https://doi.org/10.1074/jbc.M701586200
  8. M. A. Baraibar and B. Friguet, Oxidative proteome modifications target specific cellular pathways during oxidative stress, cellular senescence and aging, Exp. Gerontol., 48, 620 (2013). https://doi.org/10.1016/j.exger.2012.10.007
  9. S. Y. Tang, M. Whiteman, Z. F. Peng, A. Jenner, E. L. Yong, and B. Halliwell, Characterization of antioxidant and antiglycation properties of active ingredients from traditional chinese medicines, Free. Radic. Biol. Med., 36, 1575 (2004). https://doi.org/10.1016/j.freeradbiomed.2004.03.017
  10. S. Shin, J. A. Lee, M. Kim, H. Kum, E. Jung, and D. Park, Anti-glycation activities of phenolic constituents from Silybum marianum (milk thistle) flower in vitro and on human explants, Molecules, 20, 3549 (2015). https://doi.org/10.3390/molecules20033549
  11. H. Pageon, H. Zucchi, Z. Dai, D. R. Sell, C. M. Strauch, V. M. Monnier, and D. Asselineau, Biological effects induced by specific advanced glycation end products in the reconstructed skin model of aging, Biores. Open. Access., 4(1), 54 (2015). https://doi.org/10.1089/biores.2014.0053
  12. J. H. Kim, G. S. Sim, J. T. Bae, J. Y. Oh, G. S. Lee, D. H. Lee, B. C. Lee, and H. B. Pyo, Synthesis and anti-melanogenic effects of lipoic acid-polyethylene glycol ester, J. Pharm. Pharmacol., 60(7), 863 (2008). https://doi.org/10.1211/jpp.60.7.0007