Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effect of Gamma Ray on Germination, Growth and Antioxidant Activity of Senna tora

감마선 조사가 결명자의 생육과 항산화 활성에 미치는 영향

  • Um, Min (Division of Forest Resources, Chonnam National University) ;
  • Kang, Si Yong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Jae Won (Division of Forest Resources, Chonnam National University) ;
  • Lee, Ok Ran (Department of Plant Biotechnology, Chonnam National University)
  • 엄민 (전남대학교 산림자원학부) ;
  • 강시용 (한국원자력연구원 첨단방사선연구소) ;
  • 이재원 (전남대학교 산림자원학부) ;
  • 이옥란 (전남대학교 식물생명공학부)
  • Received : 2017.08.07
  • Accepted : 2017.10.09
  • Published : 2017.10.30
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Abstract

Background: Senna tora is a flowering plant in the legume family Fabaceae. Its seeds are roasted and consumed as tea in Asia, to reduce inflammation in the liver and improve eyesight. Thus, it has been considered as an important medicinal crops in Asia. However, breeding trials to improve its genetic properties are rare. Mutation breeding by gamma ray is known to be an effective and highly successful approach for the generation of agronomically useful cultivars. Here we analyzed the effects of several dosages of gamma ray on the biological conditions of Senna tora seeds. Methods and Results: The germination rate and growth patterns of Senna tora were examined following irradiation with gamma ray at 100, 200, 300 and 400 Gy. The total phenolic compound contents and antioxidant activities of Senna tora were analyzed. Germination increased at 100 and 200 Gy in the M1 and M2 generations compared with that of the control (M0). The total phenolic compound contents and antioxidant activity of the seeds significantly decreased as the radiation dosage increased above 100 Gy in the M1 generation. Conclusions: Senna tora, irradiated with gamma ray at dosages 100, 200, 300, and 400 Gy, showed maximum germination rate at 200 Gy in the M2 generation. Plant height and leaf size gradually decreased with increasing gamma ray intensity in the M2 generation. The total phenolic compound contents decreased significantly at 400 Gy, and the related antioxidant activity was also decreased as the radiation dosage increased.

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