Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Physicochemical components of Cynanchi wilfordii Radix fermented with Rhizopus delemar

Rhizopus delemar 발효 백수오의 이화학적 성분 분석

  • Song, Bit-Na (Fermentated Food Science Division, National Institute of Agricultural Sciences, RDA) ;
  • Kim, Eun-Ju (Fermentated Food Science Division, National Institute of Agricultural Sciences, RDA) ;
  • Jeong, Da-Som (Fermentated Food Science Division, National Institute of Agricultural Sciences, RDA) ;
  • Kim, So-Young (Fermentated Food Science Division, National Institute of Agricultural Sciences, RDA) ;
  • Cho, Yong-Sik (Fermentated Food Science Division, National Institute of Agricultural Sciences, RDA) ;
  • Park, Shin-Young (Fermentated Food Science Division, National Institute of Agricultural Sciences, RDA)
  • 송빛나 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과) ;
  • 김은주 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과) ;
  • 정다솜 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과) ;
  • 김소영 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과) ;
  • 조용식 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과) ;
  • 박신영 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과)
  • Received : 2017.09.14
  • Accepted : 2017.10.20
  • Published : 2017.10.30
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Abstract

This study carried out to determine the physicochemical components of Cynanchi wilfordii Radix (CWR) fermented with Rhizopus delemar. The physicochemical components such as changes on moisture content, pH, total acidity, reducing sugars, and the contents of free sugars, free amino acid, cynandione A were investigated. The moisture content of fermented CWR was increased after 4 days of fermentation. The pH value of fermented CWR was decreased after 4 days of fermentation. The reducing sugars contents were in the range of 1.42-1.69%. The major free sugars were identified as rhamnose, glucose, fructose and sucrose. The content of free sugars was decreased during fermentation process of CWR while the glucose contents in 80% ethanol and water extracts were increased. In addition, the free amino acid increased significantly during fermentation. Finally, The content of cynandion A of the fermented CWR was highest at the level of 14.09 mg/g on the $8^{th}$ day. These results are indicated that fermentation with Rhizopus strains could be used to increase the bioactivity of CWR and the fermented CWR can be used as a functional material and edible resource in the food industry.

본 연구에서는 발효 백수오의 이용성 및 기능성을 증진시킨 식품 및 소재를 개발하기 위한 연구의 일환으로 기초 연구를 실시하였다. 총산도는 대조구에 비해 증가하는 경향을 보였고 발효 4일차에 0.48%로 가장 높았다. 환원당 함량 측정결과 발효 4일차 백수오는 대조구 비해 3.5배 증가하는 경향을 보였다. 유리당 함량 분석결과 발효 4일차 백수오의 80% 에탄올 추출물의 경우 glucose의 함량이 대조구에 비해 약 27.7배 증가하였고, 열수추출물에서도 약 15배 증가하였다. 총 아미노산은 필수아미노산 8종을 포함해서 총 17종이 검출되었다. 배양 8일차에는 90.36 mg/100 g 으로 증가하는 경향을 보였다. 백수오의 쓴맛을 내는 아미노산 arginine은 발효기간 동안 대조구에 비해 함량이 감소하였고, 단맛을 내는 아미노산인 alanine, threonine과 감칠맛을 나타내는 aspartic acid은 증가하였다. 백수오에 존재하는 지표물질인 cynandione A를 분석한 결과 80% 에탄올 추출물과 물 추출물은 발효 8일차에 가장 높은 함량을 나타내었다. 본 연구에서 R. delemar의 발효를 통해 백수오의 기능성을 증대시킬 수 있음을 알 수 있었고, 이러한 R. delemar 균주 발효 백수오를 이용하여 기능성 및 생리활성 효능 등이 증대된 식품소재 및 건강기능식품으로 개발 가능성이 있음을 제시하였다. 따라서 본 연구결과를 기초자료로 하여 백수오의 기능성을 증대시키기에 적합한 R. delemar 균주를 이용하여 배양일에 따른 백수오의 이화학적 특성 및 성분분석과 더불어 세포실험 등을 통해 기대되는 생리활성 효능에 대한 검증이 필요할 것으로 생각된다.

Keywords

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