Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Effect of commercial sanitizers on microbial quality of fresh-cut iceberg lettuce during storage

세척용 시판 살균제 종류에 따른 신선편의 양상추의 저장 중 미생물 변화

  • Hwang, Tae-Young (Department of Food Science and Technology, Jungwon University)
  • 황태영 (중원대학교 식품공학과)
  • Received : 2017.08.02
  • Accepted : 2017.09.27
  • Published : 2017.10.30
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Abstract

This study was investigated the effects of various commercial sanitizers on microbial characteristics in fresh-cut iceberg lettuce during storage. For screening sanitizer, lettuce was cut and dipped in chlorine water ($0.2ml{\cdot}L^{-1}$), solution of organic acids such as ascorbic acid, citric acid, acetic acid, mixture of ascorbic acid and acetic acid (1-6%), and solutions of commercial sanitizers such as Formula 4$^{TM}$ (1,3,4%), Fresh produce wash$^{TM}$ (1,3,4%), Cleancol$^{TM}$ (1%), Chitochol$^{TM}$ (1%) and Natural Ca$^{TM}$ (0.1%) for 3 min, respectively. Washing lettuce with selected sanitizers resulted in reduction of aerobic bacteria of more than 2 log CFU/g. Initial pH of lettuce was related with the pH of sanitizers. pH ranged from 4.7 to 6.1 in Formula 4 (4%, pH 1.7) and Natural Ca (0.1%, pH 12.0), respectively. Chlorine water showed consistent and significant inhibition effect in all of microorganisms except total coliform. Over 3% of Formula 4 and Fresh produce wash were found to have high bactericidal activity among sanitizers. The sanitizers of chlorine water, Fresh produce wash, Chitochol and Natural Ca were effective in reducing yeast and mould populations. As coliform and E. coli, Formula 4 (4%) showed the highest bactericidal activity. The bactericidal effect of commercial sanitizers during storage varied with the kinds and concentrations of tested sanitizers. Although inhibition effect was not showed during storage, these results suggest that commercial sanitizers could be an alternative to chlorine for washing fresh-cut produce.

시판 살균제의 실제 적용 시 감균 효과를 조사하기 위해 양상추를 신선편의 가공하고, 세척 시 살균제로 이들을 적용한 후 저장 중 pH 및 일반세균, 효모, 곰팡이, 대장균, 대장균군의 변화를 조사하였다. 살균제 스크리닝 결과, 0.02%의 염소수 처리 시 3.1 log 감소를, 1% 농도에서 acetic acid는 2.4 log, ascorbic acid는 1.3, citric acid는 0.7 log의 감균 효과를 나타내었다. 시판 중인 살균제 대부분에서 2 log 의 감균 효과를 나타내어, 이들을 신선편의 양상추에 적용하고 $10^{\circ}C$에 저장하면서 양상추의 pH 변화 및 미생물의 변화를 조사하였다. 저장 초기 양상추의 pH는 살균제 용액의 pH에 따라 변화하고 있었는데, 용액의 pH가 가장 높은 칼슘제제(12.0)가 6.1, 가장 낮은 Formula 4(4%, pH 1.7)에서 가장 낮은 pH(4.7)로 나타났으며 저장일 경과에 따라 pH는 유의적으로 증가하고 있었다. 대장균군을 제외하고 0.02%의 염소수가 가장 높은 수준의 미생물 저해 효과를 나타내었다. 반면 Formula 4, Fresh produce wash 모두 3% 이상에서 미생물 저해 효과가 나타나고 있었다. 특히 Formula 4는 대장균 및 대장균군 저해 효과가 매우 좋았다. 모든 처리구에서 곰팡이는 검출되지 않았으며 효모의 경우 염소수와 Fresh produce wash 및 알코올 살균제 중 키토콜과 칼슘 제제 처리구가 효과적으로 저해하고 있었다. 시판 살균제에 따라 미생물 저해 효과는 다르게 나타나고 있었으나 염소수와 비교했을 때 초기 미생물 저해 효과는 존재하고 있기 때문에 부가적인 hurdle technology 및 공정관리를 통해 저장 중 항미생물 효과를 지속시킨다면 실제 적용이 가능할 것으로 판단된다.

Keywords

References

  1. Hwang TY, Moon KD (2005) Technical trend and prospect of minimal processing fruits and vegetables industry. Food Science and Industry, 38, 120-130
  2. KFDA (2007) Notification No. 2007-63, Korea Food and Drug Administration, Seoul, Korea, p 29-31
  3. Gil MI, Selma MV, Lopez-Galvez F, Allende A (2009) Fresh-cut product sanitation and wash water disinfection: Problems and solutions. Int J Food Microbiol, 134, 37-45 https://doi.org/10.1016/j.ijfoodmicro.2009.05.021
  4. Rico D, Martin-Diana AB, Barat JM, Barry-Ryan C (2007) Extending and measuring the quality of fresh-cut fruit and vegetables: A review. Trends Food Sci Technol, 18, 373-386 https://doi.org/10.1016/j.tifs.2007.03.011
  5. Francis GA, Thomas C, O'beirne D (1999) The microbiological safety of minimally processed vegetables. Int J Food Sci Technol, 34, 1-22 https://doi.org/10.1046/j.1365-2621.1999.00253.x
  6. Nou XW, Luo YG (2010) Whole-leaf wash improves chlorine efficacy for microbial reduction and prevents pathogen cross-contamination during fresh-cut lettuce processing. J Food Sci, 75, 283-290 https://doi.org/10.1111/j.1750-3841.2010.01630.x
  7. Van Haute S, Sampers I, Holvoet K, Uyttendaele M (2013) Physicochemical quality and chemical safety of chlorine as a reconditioning agent and wash water disinfectant for fresh-cut lettuce washing. Appl Environ Microbiol, 79, 2850-2861 https://doi.org/10.1128/AEM.03283-12
  8. Ongeng D, Devlieghere F, Coosemans J, Ryckeboer J (2006) The efficacy of electrolysed oxidising water for inactivating spoilage microorganisms in process water and on minimally processed vegetables. Int J Food Microbiol, 109, 187-197 https://doi.org/10.1016/j.ijfoodmicro.2005.12.013
  9. Zhang S, Farber JM (1996) The effects of various disinfectants against Listeria monocytogenes on fresh-cut vegetables. Food Microbiol, 13, 311-321 https://doi.org/10.1006/fmic.1996.0037
  10. Petri E, Rodriguez M, Garcia S (2015) Evaluation of combined disinfection methods for reducing Escherichia coli O157:H7 population on fresh-cut vegetables. Int J Environ Res Public Health, 12, 8678-8690 https://doi.org/10.3390/ijerph120808678
  11. Akbas MY, Olmez H (2007) Inactivation of Escherichia coli and Listeria monocytogenes on iceberg lettuce by dip wash treatments with organic acids. Lett Appl Microbiol, 44, 619-624 https://doi.org/10.1111/j.1472-765X.2007.02127.x
  12. Breidt Jr F, Hayes JS, McFeeters RF (2004) Independent effects of acetic acid and pH on survival of Escherichia coli in simulated acidified pickle products. J Food prot, 67, 12-18 https://doi.org/10.4315/0362-028X-67.1.12
  13. Dickson JS (1992) Acetic acid action on beef tissue surfaces contaminated with Salmonella typhimurium. J Food Sci, 57, 297-301 https://doi.org/10.1111/j.1365-2621.1992.tb05480.x
  14. Ahvenainen R (1996) New approaches in improving the shelf life of minimally processed fruit and vegetables. Trends Food Sci Technol, 7, 179-187 https://doi.org/10.1016/0924-2244(96)10022-4
  15. Ke D, Mateos M, Siriphanich J, Li C, Kader AA (1993) Carbon dioxide action on metabolism of organic and amino acids in crisphead lettuce. Postharvest Biol Technol, 3, 235-247 https://doi.org/10.1016/0925-5214(93)90059-C
  16. Tirpanalan O, Zunabovic M, Domig KJ, Kneifel W (2011) Mini review: Antimicrobial strategies in the production of fresh-cut lettuce products. In: Science against microbial pathogens: Communicating current research and technological advances, Mendez-Vilas A (Editor), Formatex Research Center, Bodajoz, Spain, p 176-188
  17. Ita PS, Hutkins RW (1991) Intracellular pH and survival of Listeria monocytogenes Scott A in tryptic soy broth containing acetic, lactic, citric, and hydrochloric acids. J Food Prot, 54, 15-19 https://doi.org/10.4315/0362-028X-54.1.15
  18. Booth IR (1985) Regulation of cytoplasmic pH in bacteria. Microbiol Rev, 49, 359-378
  19. Ouattara B, Simard RE, Holley RA, Piette GJP, Begin A (1997) Inhibitory effect of organic acids upon meat spoilage bacteria. J Food Prot, 60, 246-253 https://doi.org/10.4315/0362-028X-60.3.246
  20. Young KM, Foegeding PM (1993) Acetic, lactic, and citric acids and pH inhibition of Listeria monocytogenes Scott A and the effect on intracellular pH. J Appl Bacteriol, 74, 515-520
  21. Ingram LO (1981) Mechanism of lysis of Escherichia coli by ethanol and other chaotpic agents. J Bacteriol, 146, 331-336
  22. Tamblyn KC, Conner DE (1997) Bactericidal activity of organic acids in combination with transdermal compounds against Salmonella typhimurium attached to broiler skin. Food Microbiol, 14, 477-484 https://doi.org/10.1006/fmic.1997.0112
  23. Hassan R, El-Kadi S, Sand M (2015) Effect of some organic acids on some fungal growth and their toxins production. Int J Adv Biol, 2, 1-11 https://doi.org/10.5121/ijab.2015.2401