Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant and anti-inflammatory effects of phenolic rich Hylotelephium erythrostictum extracts

폴리페놀 함량이 높은 Hylotelephium erythrosticum 추출물의 항산화 및 항염 활성

  • Kim, Sang Suk (Citrus Research Institute, National Institute of Horticultural and Herbal Science, RDA) ;
  • Park, Kyung Jin (Citrus Research Institute, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Seung Eun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Jeong Hoon (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Choi, Young Hun (Citrus Research Institute, National Institute of Horticultural and Herbal Science, RDA)
  • 김상숙 (국립원예특작과학원 감귤연구소) ;
  • 박경진 (국립원예특작과학원 감귤연구소) ;
  • 이승은 (국립원예특작과학원 인삼특작부) ;
  • 이정훈 (국립원예특작과학원 인삼특작부) ;
  • 최영훈 (국립원예특작과학원 감귤연구소)
  • Received : 2017.09.08
  • Accepted : 2017.10.11
  • Published : 2017.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

Hylotelephium erythrostictum is commonly used as a medicinal herb. In this study, H. erythrostictum leaf (HEL), branch (HEB), root (HER), and above ground (HEAG) extracts were evaluated for their antioxidant properties. The antioxidant activities were assayed by three methods based on scavenging of DPPH, ABTS and superoxide anion radical. HEAG extract showed the highest DPPH, ABTS, superoxide anion radical scavenging activities. HEAG extract also exhibited the highest phenolic content (230 mg/g gallic acid equivalent). In our research for anti-inflammatory ingredients, the extract of HEAG inhibited the generation of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. To test the inhibitory effects of HEAG on pro-inflammatory cytokines, we conducted ELISA assay for the measuring the generation of tumor necrosis factor $(TNF)-{\alpha}$, IL (interleukin)-$1{\beta}$, and IL(interleukin)-6 in LPS-stimulated RAW264.7 macrophage cells. In these assays, HEAG ethanol extract showed a dose-dependent decrease in the production of $TNF-{\alpha}$, $IL-1{\beta}$, and IL-6. Based on these results, extract of HEAG could be the efficient candidate for anti-inflammatory agents.

피부병 치료에 사용되던 꿩의비름 추출물을 항산화 및 항염 효능을 지니는 천연물 소재로 활용하기 위해 꿩의비름의 뿌리(HER), 잎(HEL), 줄기(HEB), 지상부(HEAG) 추출물에 대해 항산화 활성을 측정한 결과 지상부 추출물인 HEAG에서 DPPH, ABTS, superoxide anion radical 소거 활성이 가장 우수하였으며, FRAP 활성 역시 HEAG에서 가장 우수하였다. 항산화 활성이 가장 우수한 HEAG의 총 폴리페놀 함량을 측정한 결과 $235.5{\pm}18.96mg/g$ gallic acid equivalent로 항산화 및 항염 활성을 갖는 것으로 알려진 페놀성 화합물을 다량 함유하고 있는 것을 확인할 수 있었다. 또한 마우스 대식세포인 RAW264.7 세포를 이용하여 항염 활성에서는 NO의 생성을 억제하였으며 염증성 사이토카인인 $IL-1{\beta}$의 생성 억제 화성이 가장 높았다. 꿩의비름 추출물은 항산화 및 항염 활성의 천연물 소재로 활용이 가능할 것으로 여겨진다.

Keywords

References

  1. Nanes MS (2003) Tumor necrosis factor-${\alpha}$: molecular and cellular mechanisms in skeletal pathology. Gene, 321, 1-15 https://doi.org/10.1016/S0378-1119(03)00841-2
  2. Jeon AL, Kim JE, Lee NH (2017) Whitening and anti-inflammatory constitutes from the extract of Citrullus lanatus vines. J Soc Cosmet Sci Korea, 53-60
  3. Kim, SS, Oh HJ, Baik JS, Oh TH, Yun PY, Kim CS, Lee NH, Hyun CG (2008) Chemical composition and biological activities of Elsholtzia splendens essential oil. J Appl Biol Chem, 51, 69-72 https://doi.org/10.3839/jabc.2008.013
  4. Kim SS, Hyun CG, Choi YH, Lee NH (2013) Tyrosinase inhibitory activities of the compounds isolated from Neolitsea aciculata (Blume) Koidz. J Enzyme Inhib Med Chem, 28, 685-689 https://doi.org/10.3109/14756366.2012.670806
  5. Shih MF, Cheng YD, Shen CR, Cherng JY (2010) A molecular pharmacology study into the anti-inflammatory actions of Euphorbia hirta L. on the LPS-induced RAW 264.7 cells through selective iNOS protein inhibition. J Nat Med, 64, 330-335 https://doi.org/10.1007/s11418-010-0417-6
  6. Tsatsanis C, Androulidaki A, Venihaki M, Margioris AN (2006) Signalling networks regulating cyclooxygenase-2. Int J Biochem Cell Biol, 38, 1654-1661 https://doi.org/10.1016/j.biocel.2006.03.021
  7. Kim MJ, Kim SS, Park KJ, An HJ, Choi YH, Lee NH, Hyun CG (2016) Methyl j asmonate inhibits lipopolysaccharide-induced inflammatory cytokine production via mitogen-activated protein kinase and nuclear factor-${\kappa}B$ pathways in RAW 264.7 cells. Die Pharmazie, 71, 540-543
  8. Ham YM, Yoon WJ, Lee WJ, Kim SC, Baik JS, Kim JH, Lee GS, Lee NH, Hyun CG (2015) Anti-inflammatory effects of isoketocharbroic acid from brown alga, Sargassum micracanthum. EXCLI J, 14, 1116-1121
  9. Yoon ES (1997) Effect of plant growth regulators on plant regeneration from leaf and stem explant cultures of Sedum erythrostichum Miq.. Korean J Plant Tissue Culture, 24, 285-289
  10. Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1200 https://doi.org/10.1038/1811199a0
  11. Liu F, Ooi VEC, Chang ST (1997) Free radical scavenging activity of mushroom polysaccharide extracts. Life Sci, 60, 763-771 https://doi.org/10.1016/S0024-3205(97)00004-0
  12. Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol, 299, 152-178
  13. Nieva Moreno MI, Isla MI, Sampietro AR, Vattuone MA (2000) Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol, 71, 109-114 https://doi.org/10.1016/S0378-8741(99)00189-0
  14. Devasagayam TPA, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD (2004) Free radicals and antioxidants in human health: Current status and future prospects. J Assoc Physicians India, 52, 794-804
  15. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol, 39, 44-84 https://doi.org/10.1016/j.biocel.2006.07.001
  16. Maeda H, Akaike T (1998) Nitric oxide and oxygen radicals in infection, inflammation, and cancer. Biochemistry (Mosc), 63, 854-865
  17. Samak G, Shenoy RP, Manjunatha SM, Vinayak KS (2009) Superoxide and hydroxyl radical scavenging actions of botanical extracts of Wagatea spicata. Food Chem, 115, 631-634 https://doi.org/10.1016/j.foodchem.2008.12.078
  18. Gulcin I, Berashvili D, Gepdiremen A (2005) Antiradical and antioxidant activity of total anthocyanins from Perilla pankinensis decne. J Ethmopharmacol, 101, 287-293 https://doi.org/10.1016/j.jep.2005.05.006
  19. Joung YM, Park SJ, Lee KY, Lee JY, Suh JK, Hwang SY, Park KE, Kang MH (2007) Antioxidant and antimicrobial activities of Lilium species extracts prepared from different aerial parts. Korean J Food Sci Technol, 39, 452-457
  20. Choi JS, Kim HY, Seo WT, Lee JH, Cho KM (2012) Roasting enhances antioxidant effect of bitter melon (Momordica charantia L.) increasing in flavan-3-ol and phenolic acid contents. Food Sci Biotechnol, 21, 19-26 https://doi.org/10.1007/s10068-012-0003-7
  21. Kim SS, Park KJ, An HJ, Choi YH (2017) Phytochemical, antioxidant, and antibacterial activities of fermented Citrus unshiu byproduct. Food Sci Biotechnol. 26, 461-466 https://doi.org/10.1007/s10068-017-0063-9
  22. Kwak JH, Choi GN, Park JH, Kim JH, Jeong HR, Jeong CH, Heo HJ (2010) Antioxidant and neuronal cell protective effect of purple sweet potato extract. J Agriculture Life Sciences, 44, 57-66
  23. Lee SY, Shin YJ, Park JH, Kim SM, Park CS (2008) An analysis of the Gyungokgo's ingredients and a comparison study on anti-oxidation effects according to the kinds of extract. Kor J Herbology, 23, 123-136
  24. Kang MH, Choi CS, Kim ZS, Chung HK, Min KS, Park CG, Park HW (2002) Antioxidative activities of ethanol extract prepared from leaves, seed, branch and aerial part of Crotalaria sessiflora L.. Korean J Food Sci Technol, 34, 1098-1102
  25. Yoon WJ, Lee JA, Kim KN, Kim JY, Park SY (2007) In vitro anti-inflammatory activity of the Artemisia fukudo extracts in murine macrophage RAW 264.7 cells. Korean J Food Sci Technol, 39, 464-469
  26. Fenton MJ, Golenbock DT (1998) LPS-binding proteins and receptors. J Leukocyte Biol, 64, 25-32 https://doi.org/10.1002/jlb.64.1.25
  27. Shih MF, Cheng YD, Shen CR, Cherng JY (2010) A molecular pharmacology study into the anti-inflammatory actions of Euphorbia hirta L. on the LPS-induced RAW 264.7 cells through selective iNOS protein inhibition. J Nat Med, 64, 330-335 https://doi.org/10.1007/s11418-010-0417-6
  28. Choe SI, Jo MJ, Kim SC, Byun SH (2008) Inhibitory effect of Sunbanghwalmyungeum MeOH extract on pro-inflammatory mediator in lipopolysaccharide-activated Raw 264.7 cells. Kor J Herbology, 23, 11-18

Cited by

  1. Chemical Constituents of Hylotelephium erythrostictum vol.57, pp.6, 2021, https://doi.org/10.1007/s10600-021-03571-z