Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Radical scavenging and α-glucosidase inhibitory effects of Mongolian Iris bungei extract

몽골산 Iris bungei 추출물의 자유 라디칼 소거 및 α-glucosidase 저해 활성

  • Jeong, Yun Hee (Department of Food Science and Biotechnology, Daegu University) ;
  • Jeong, Gyeong Han (Department of Food Science and Biotechnology, Daegu University) ;
  • Kim, Tae Hoon (Department of Food Science and Biotechnology, Daegu University)
  • 정윤희 (대구대학교 식품공학과) ;
  • 정경한 (대구대학교 식품공학과) ;
  • 김태훈 (대구대학교 식품공학과)
  • Received : 2017.08.22
  • Accepted : 2017.09.25
  • Published : 2017.10.30
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Abstract

In a continuing screening of selected medicinal plants native to Mongolia, the antioxidant and ${\alpha}$-glucosidase inhibitory activities of methanol extract of Iris bungei were investigated. After extraction with 80% of methanol, the methanol fraction was further extracted with n-hexane, EtOAc and n-BuOH in order to obtain four different solvent-soluble fractions, namely n-hexane-soluble, EtOAc-soluble, n-BuOH-soluble and $H_2O$ residue. The antioxidant properties were evaluated by radical scavenging assay using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) ($ABTS^+$) radicals. The anti-diabetic efficacy of I. bungei extract was investigated by ${\alpha}$-glucosidase assay. All tested samples showed dose-dependent radical scavenging and ${\alpha}$-glucosidase inhibitory activities. Among the tested extracts, the EtOAc-soluble fractions showed the greatest radical scavenging activity and ${\alpha}$-glucosidase inhibitory properties among other solvent-soluble fractions. This result suggested that there was a significant relationship between the total phenolic content and biological efficacy. Thus, I. bungei extract might be considered as a new potential source of natural antioxidant and as a ${\alpha}$-glucosidase inhibitory source. A more systematic investigation of this biomass sill be performed for further investigation of activity against antioxidative and anti-diabetic effects.

몽골산 I. bungei를 80% 메탄올로 침지 추출하여 얻어진 추출물에 대해 n-hexane, EtOAc 및 n-BuOH의 유기용매를 활용하여 순차 분획을 실시하였고, 얻어진 결과물에 대하여 총 페놀함량, DPPH, $ABTS^+$ 라디칼 소거능 및 ${\alpha}$-glucosidase 저해활성을 평가하였다. DPPH 라디칼 소거활성은 페놀성 화합물의 함량이 높은 EtOAc 가용 분획물에서 $IC_{50}$ 값이 $148.7{\pm}1.6{\mu}g/mL$으로 우수한 DPPH 라디칼 소거 활성을 확인하였고, $ABTS^+$ 라디칼 소거활성 역시 I. bungei의 EtOAc 가용 분획물에서 $IC_{50}$ 값이 $27.8{\pm}0.9{\mu}g/mL$의 매우 우수한 라디칼 소거활성을 확인하였고, 다량 존재하는 페놀성 화합물과 라디칼 소거활성의 관련성을 나타내었다. 또한 ${\alpha}$-glucosidase 저해활성을 평가한 결과 EtOAc 가용 분획물에서 $IC_{50}$값은 $4.6{\pm}0.1{\mu}g/mL$의 강력한 저해활성을 나타내었으며, 이는 양성 대조군인 acarbose의 $IC_{50}$ 값인 $473.2{\pm}5.5{\mu}g/mL$과 비교했을 때 매우 우수한 활성을 나타내었으며, 다양한 화합물이 함유된 상태의 추출물 시료를 단일물질로 정제할 경우 더욱 우수한 활성의 화합물이 존재할 가능성을 기대하게 한다. 향후 이들 추출물의 활성물질 동정을 통한 활성 기작에 대한 연구가 필요하며, 본 연구결과로 보다 우수한 라디칼 소거활성 및 ${\alpha}$-glucosidase 저해활성을 가지는 새로운 천연 기능성 소재 발굴을 위한 기초자료로 활용 가능할 것으로 사료된다.

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