Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
권호 표지
DOI QR코드

DOI QR Code

Rapid Analysis of Nitrate Concentration in Different Growth Stages and Plant Parts of Paprika Leaf Using On-site Rapid Detection Kit

신속분석기기를 이용한 파프리카 생육단계 및 부위별 엽내 질산태질소 농도 신속분석

  • Lee, Min Ji (Department of Horticulture, College of Agriculture & Life Sciences, Chonbuk National University) ;
  • Rhee, Han Cheol (Protected Horticulture Research Station, National Institute of Horticulture & Herbal Science, Rural Development Administration) ;
  • Choi, Gyeong Lee (Protected Horticulture Research Station, National Institute of Horticulture & Herbal Science, Rural Development Administration) ;
  • Oh, Sang Seok (Gyeongnam Agricultural Research & Extension Services) ;
  • Lee, Jae Taek (Fruit Vegetable Research Institute, Jeonbuk ARES.) ;
  • Lee, Jun Gu (Department of Horticulture, College of Agriculture & Life Sciences, Chonbuk National University)
  • 이민지 (전북대학교 원예학과) ;
  • 이한철 (국립원예특작과학원 시설원예연구소) ;
  • 최경이 (국립원예특작과학원 시설원예연구소) ;
  • 오상석 (경상남도농업기술원 미래농업교육과) ;
  • 이재택 (전라북도농업기술원 과채류연구소) ;
  • 이준구 (전북대학교 원예학과)
  • Received : 2017.08.11
  • Accepted : 2017.10.11
  • Published : 2017.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

This research was aimed to establish rapid analysis technique for the determination of nitrate ($NO_3{^-}$) concentration in the leaves of paprika, which has key role for the stable vegetative and reproductive growth. Leaf petiole and blade sap of two paprika cultivars ('Raon red' and 'Raon yellow') were used for the determination of $NO_3{^-}$ concentration, separately using rapid detection kit (RQ-flex) and spectroscopy quantification methods. In addition, two paprika cultivars namely, 'Nicole' and 'TP2001' were used to determine the status of $NO_3{^-}$ concentration in leaf of each fruiting group. $NO_3{^-}$ concentration in leaf blade sap and the content in leaf showed significant correlation ($R^2=0.8628$), analysed by RQ-flex and spectroscopy methods, respectively. Furthermore, leaf petiole sap and the content in leaf also showed significant correlation ($R^2=0.6734$) but the relationship was poor compared to leaf blade sap and the leaf content. $NO_3{^-}$ concentration in petiole sap decreased in all the cultivars from early to late fruiting group. The higher concentration in the lower leaves and the continuous decrease towards the upper leaves in the both years were found through the analysis of $NO_3{^-}$ concentration in different leaf position. In addition, daily short-term fluctuation of $NO_3{^-}$ in petiole sap could be rapidly monitored. These results showed that long-term or short-term monitoring by test strip-based rapid analysis technique might be useful tool for the diagnosis of nutritional status for the stable of nutritional management in paprika.

본 연구는 겨울철 파프리카의 안정된 영양생장, 생식생장을 파악하는 방법으로 간이영양진단기기를 이용해 $NO_3{^-}$ 농도를 신속하게 측정하고 영양상태 파악을 위해 실시되었다. 'Raon red', 'Raon yellow' 두 품종을 각각 비대기 단계로 나누어 측정한 결과 엽신의 test strip 분석은 분광광도계를 통한 관행의 방법과 상관성이 인정되었고($R^2=0.8628$), 엽병의 test strip분석은 엽신 즙액 보다는 낮은 상관성을 보이지만 간접적으로 $NO_3{^-}$ 농도 분석에 유용하였다($R^2=0.6734$). 착과 그룹이 높아짐에 따라 해당 부위 엽 내 질산태질소 농도가 낮아지는 경향을 보이고 있으며, 동일 시점의 마디별 엽병 즙액 내 질산태질소 농도 역시 정단부로 갈수록 낮아지는 경향이 확인되었다. 엽병 즙액 내 $NO_3{^-}$ 농도 역시 간이진단기기를 활용하여 신속한 모니터링이 가능하였다. 따라서 간이영양진단기기를 사용해 기존 분석방법보다 효과적으로 농가 현장에서 파프리카의 질소 영양상태를 파악할 수 있었으며, 이를 통해 추후 합리적인 양액공급 및 표준영양범위의 설정이 가능할 것으로 판단된다.

Keywords

References

  1. Bennett, W.F. 1993. Nutrient deficiencies and toxicities in crop plants. APS Press, St. Paul, USA.
  2. Cataldo, D.A., M. Maroon, L.E. Schrader, and V.L. Youngs. 1975. Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Commun. Soil Sci. Plant Anal. 6:71-80. https://doi.org/10.1080/00103627509366547
  3. Del Amor, F.M. 2006. Growth, photosynthesis and chlorophyll fluorescence of sweet pepper plants as affected by the cultivation method. Ann. Appl. Biol. 148:133-139. https://doi.org/10.1111/j.1744-7348.2006.00048.x
  4. Heuvelink, E., L.F.M. Marcelis, and O. Korner. 2004. How to reduce yield fluctuations in sweet pepper? Acta Hort. 633:349-355.
  5. Hochmuth, G.J. 1994. Efficiency ranges for nitrate-nitrogen and potassium for vegetable petiole sap quick tests. Hort. Technol. 4:218-222.
  6. Husni M.H.A., A.S.N. Akmar, C.C. Law, and A.R. Zaharah. 2014. Leaf nitrogen content in oil palm seedlings and their relationship to SPAD chlorophyll meter readings. J. Oil Palm Res. Environ Health 5:8-17.
  7. Islam M.S., M.S.U. Bhuiya, S. Rahman, and M.M. Hussain. 2009. Evaluation of SPAD and LCC based nitrogen management in rice (Oryza sativa L.). Bangladesh J. Agric. Res. 34:661-672.
  8. Islam M.R., K.S. Haque, N. Akter, and M.A. Karim. 2014. Leaf chlorophyll dynamics in wheat based on SPAD meter reading and its relationship with grain yield. Sci. Agri. 8:13-18.
  9. Jeong, W.J., I.K. Kang, J.Y. Lee, S.H. Park, H.S. Kim, D.J. Myoung, G.T. Kim, and J.H. Lee. 2008. Study of dry and bio-mass of sweet pepper fruit and yield between glasshouse and plastic greenhouse. Kor. Soc. Bio-Environ. Contr. 17:541-544.
  10. Korea Agricultural Trade Information (KATI). 2014. The state of sweet pepper industry in Korea. Korean Agro-Fisheries Trade Corporation, Seoul, Korea.
  11. Korea Agricultural Trade Information (KATI). 2015. The state of sweet pepper industry in Korea. Korean Agro-Fisheries Trade Corporation, Seoul, Korea.
  12. Kim, J.Y. 2011. Rapid and simple methods of nutrient analysis for sweet pepper (Capsicum annuum L.) M.S Thesis. University of Seoul Univ. Seoul, Korea.
  13. Kim, K.R, and K.H. Kim. 2003. Rapid nutrient diagnosis of cucumber by test strip and chlorophyll meter. Kor. J. Soil Sci. Fertil. 36:272-279.
  14. Kim, K.R., H.U. Jeoung, and K.H. Kim. 2003. Rapid nutrient diagnosis of tomato by test strips and a chlorophyll meter. J. Kor. Soc. Agric. Chem. Biotechnol. 46:140-143.
  15. Lim, G.H., M.N. Lee, H.J. Kim, J.H. Yoon, and H.K. Kim. 2012. Nitrogen, phosphorus, potassium diagnosis of strawberry 'Maehyang' using rapid and simple methods. Kor. J. Soil Sci. Fertil. 128-129.
  16. Studstill, D.W., E.H. Simonne, C.M. Hutchinson, R.C. Hochmuth, M.D. Dukes, and W.E. Davis. 2006. Petiole sap testing sampling procedures for monitoring pumpkin nutritional status. Commun. Soil Sci. Plant Anal. 34:2355-2362.
  17. Smith, J.H., J.C. Silvertooth, and E.R. Norton. 1998. Comparison of the two methods for the analysis of petiole nitrate nitrogen concentration in irrigated cotton. Cotton: A College of Agriculture Report 476-479.
  18. Taber, H.G, and V. Lawson. 2007. Use of diluted tomato petiole sap for potassium measurement with the cardy electrode meter. Commun. Soil Sci. Plant Anal. 38:713-718. https://doi.org/10.1080/00103620701220627
  19. Waskom, R.M., D.G. Westfall, D.E. Spellman, and P.N. Soltanpour. 1996. Monitoring nitrogen status of corn with a portable chlorophyll meter. Commun. Soil Sci. Plant Anal. 27:545-560. https://doi.org/10.1080/00103629609369576
  20. Won, J.H., B.C. Jeong, J.K. Kim, and S.J. Jeon. 2009. Selection of suitable cultivars for the hydropoincs of sweet pepper (Capsicum annuum L.) in the alpine area in summer. J. Bio-Env. Cont. 18:425-430.
  21. Zheng H.L., Y.C. Liu, Y.L. Qin, C.H.E.N. Yang, and M.S. Fan. 2015. Establishing dynamic thresholds for potato nitrogen status diagnosis with the SPAD chlorophyll meter. J. Integr. Agric. 14:190-195. https://doi.org/10.1016/S2095-3119(14)60925-4