Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Polymorphism in the intron 20 of porcine O-linked N-acetylglucosamine transferase

  • Kim, Jong Gug (Department of Animal Sciences, College of Agriculture and Life Science, and Institute of Molecular Biology and Genetics, Chonbuk National University) ;
  • Nonneman, Dan (U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center) ;
  • Kim, Doo-Wan (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Shin, Sangsu (Department of Animal Biotechnology, Kyungpook National University) ;
  • Rohrer, Gary A. (U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center)
  • Received : 2017.03.01
  • Accepted : 2017.05.22
  • Published : 2017.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of O-GlcNAc and GlcNAcylation has extensive crosstalk with phosphorylation to regulate signaling and transcription. Pig OGT is located near the region of chromosome X that affects follicle stimulating hormone level and testes size. The objective of this study was to find the variations of OGT between European and Chinese pigs. Methods: Pigs were tested initially for polymorphism in OGT among European and Chinese pigs by polymerase chain reaction and sequencing at the U.S. Meat Animal Research Center (USMARC). The polymorphism was also determined in an independent population of pigs including European and Chinese Meishan (ME) breeds at the National Institute of Animal Science (NIAS, RDA, Korea). Results: The intron 20 of OGT from European and Chinese pigs was 514 and 233 bp, respectively, in the pigs tested initially. They included 1 White composite (WC) boar and 7 sows ($2Minzu{\times}WC$, $2Duroc\;[DU]{\times}WC$, $2ME{\times}WC$, $1Fengzing{\times}WC$) at USMARC. The 281-bp difference was due to an inserted 276-bp element and GACTT in European pigs. When additional WC and ME boars, the grandparents that were used to generate the $1/2ME{\times}1/2WC$ parents, and the 84 boars of 16 litters from mating of $1/2ME{\times}1/2WC$ parents were analyzed, the breeds of origin of X chromosome quantitative trait locus (QTL) were confirmed. The polymorphism was determined in an independent population of pigs including DU, Landrace, Yorkshire, and ME breeds at NIAS. OGT was placed at position 67 cM on the chromosome X of the USMARC swine linkage map. Conclusion: There was complete concordance with the insertion in European pigs at USMARC and NIAS. This polymorphism could be a useful marker to identify the breed of origin of X chromosome QTL in pigs produced by crossbreeding Chinese and European pigs.

Keywords

References

  1. Butkinaree C, Park K, Hart GW. O-linked beta-N-acetylglucosamine (O-GlcNAc): Extensive crosstalk with phosphorylation to regulate signaling and transcription in response to nutrients and stress. Biochim Biophys Acta 2010;1800:96-106. https://doi.org/10.1016/j.bbagen.2009.07.018
  2. Bullen JW, Balsbaugh JL, Chanda D, et al. Cross-talk between two essential nutrient-sensitive enzymes: O-GlcNAc transferase (OGT) and AMP-activated protein kinase (AMPK). J Biol Chem 2014;289: 10592-606. https://doi.org/10.1074/jbc.M113.523068
  3. Hartweck LM, Scott CL, Olszewski NE. Two O-linked N-acetylglucosamine transferase genes of Arabidopsis thaliana L. Heynh. have overlapping functions necessary for gamete and seed development. Genetics 2002;161:1279-91.
  4. Dehennaut V, Lefebvre T, Leroy Y, et al. Survey of O-GlcNAc level variations in Xenopus laevis from oogenesis to early development. Glycoconj J 2009;26:301-11. https://doi.org/10.1007/s10719-008-9166-0
  5. Shafi R, Iyer SP, Ellies LG, et al. The O-GlcNAc transferase gene resides on the X chromosome and is essential for embryonic stem cell viability and mouse ontogeny. Proc Natl Acad Sci USA 2000;97:5735-9. https://doi.org/10.1073/pnas.100471497
  6. Howerton CL, Morgan CP, Fischer DB, Bale TL. O-GlcNAc transferase (OGT) as a placental biomarker of maternal stress and reprogramming of CNS gene transcription in development. Proc Natl Acad Sci USA 2013;110:5169-74. https://doi.org/10.1073/pnas.1300065110
  7. Shibutani M, Mori T, Miyano T, Miyake M. Removal of O-GlcNAcylation is important for pig preimplantation development. J Reprod Dev 2015;61:341-50. https://doi.org/10.1262/jrd.2014-173
  8. Rohrer GA, Ford JJ, Wise TH, Vallet JL, Christenson RK. Identification of quantitative trait loci affecting female reproductive traits in a multigeneration Meishan-White composite swine population. J Anim Sci 1999;77:1385-91. https://doi.org/10.2527/1999.7761385x
  9. Jiang Z, Robinson JAB, Verrinder Gibbins AM, et al. Mapping of QTLs for prolificacy traits on SSC8 using a candidate gene approach. In: 7th World Congress on Genetics Applied to Livestock Production; 2002 August 19-23; Montpellier, France; 2002.
  10. King AH, Jiang Z, Gibson JP, Haley CS, Archibald AL. Mapping quantitative trait loci affecting female reproductive traits on porcine chromosome 8. Biol Reprod 2003;68:2172-9. https://doi.org/10.1095/biolreprod.102.012955
  11. Rohrer GA, Wise TH, Lunstra DD, Ford JJ. Identification of genomic regions controlling plasma FSH concentrations in Meishan-White Composite boars. Physiol Genomics 2001;6:145-51. https://doi.org/10.1152/physiolgenomics.2001.6.3.145
  12. Kim JG, Vallet JL, Rohrer GA, Christenson RK. Characterization of porcine uterine estrogen sulfotransferase. Domest Anim Endocrinol 2002;23:493-506. https://doi.org/10.1016/S0739-7240(02)00172-8
  13. Kim JG, Vallet JL, Christenson RK. Molecular cloning and endometrial expression of porcine amphiregulin. Mol Reprod Dev 2003;65:366-72. https://doi.org/10.1002/mrd.10314
  14. Thorson JF, Desaulniers AT, Lee C, et al. The role of RFamide-related peptide 3 (RFRP3) in regulation of the neuroendocrine reproductive and growth axes of the boar. Anim Reprod Sci 2015;159:60-5. https://doi.org/10.1016/j.anireprosci.2015.05.013
  15. Kim JG, Ford JJ, Rohrer GA, Nonneman DJ. Molecular cloning of porcine OGT cDNA and mapping to the X chromosome. Biology of Reproduction, Society for the Study of Reproduction Meeting 2006; Supplement:154.
  16. Nam YS, Kim DW, Kim MJ, Cho KH, Kim JG. Length polymorphism in OGT between Korean native pig, Chinese Meishan, and the Western pig breeds. J Anim Sci Technol 2015;57:12. https://doi.org/10.1186/s40781-015-0045-5
  17. Estecio MR, Gallegos J, Dekmezian M, et al. SINE retrotransposons cause epigenetic reprogramming of adjacent gene promoters. Mol Cancer Res 2012;10:1332-42. https://doi.org/10.1158/1541-7786.MCR-12-0351
  18. Wang M, Marin A. Characterization and prediction of alternative splice sites. Gene 2006;366:219-27. https://doi.org/10.1016/j.gene.2005.07.015
  19. Bischoff SR, Tsai SQ, Hardison NE, et al. Differences in X-chromosome transcriptional activity and cholesterol metabolism between placentae from swine breeds from Asian and Western origins. PLoS One 2013; 8:e55345. https://doi.org/10.1371/journal.pone.0055345
  20. Olivier-Van Stichelen S, Abramowitz LK, Hanover JA. X marks the spot: does it matter that O-GlcNAc transferase is an X-linked gene? Biochem Biophys Res Commun 2014;453:201-7. https://doi.org/10.1016/j.bbrc.2014.06.068
  21. Groenen MA, Archibald AL, Uenishi H, et al. Analyses of pig genomes provide insight into porcine demography and evolution. Nature 2012; 491:393-8. https://doi.org/10.1038/nature11622
  22. Ford JJ, Wise TH, Lunstra DD, Rohrer GA. Interrelationships of porcine X and Y chromosomes with pituitary gonadotropins and testicular size. Biol Reprod 2001;65:906-12. https://doi.org/10.1095/biolreprod65.3.906
  23. Rohrer GA, Alexander LJ, Keele JW, Smith TP, Beattie CW. A microsatellite linkage map of the porcine genome. Genetics 1994;136:231-45.