Asian-Australasian Journal of Animal Sciences

  • 제30권11호
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  • Pages.1575-1589
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  • 2017
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Carbohydrate and lipid spectroscopic molecular structures of different alfalfa hay and their relationship with nutrient availability in ruminants

  • Yari, Mojtaba (Department of Animal and Poultry Science, University of Saskatchewan) ;
  • Valizadeh, Reza (Department of Animal Science, Ferdowsi University of Mashhad) ;
  • Nnaserian, Abbas Ali (Department of Animal Science, Ferdowsi University of Mashhad) ;
  • Jonker, Arjan (Department of Animal and Poultry Science, University of Saskatchewan) ;
  • Yu, Peiqiang (Department of Animal and Poultry Science, University of Saskatchewan)
  • 투고 : 2016.09.28
  • 심사 : 2017.03.06
  • 발행 : 2017.11.01
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초록

Objective: This study was conducted to determine molecular structures related to carbohydrates and lipid in alfalfa hay cut at early bud, late bud and early flower and in the afternoon and next morning using Fourier transform infrared spectroscopy (FT/IR) and to determine their relationship with alfalfa hay nutrient profile and availability in ruminants. Methods: Chemical composition analysis, carbohydrate fractionation, in situ ruminal degradability, and DVE/OEB model were used to measure nutrient profile and availability of alfalfa hay. Univariate analysis, hierarchical cluster analysis (CLA) and principal components analysis (PCA) were conducted to identify FT/IR spectra differences. Results: The FT/IR non-structural carbohydrate (NSCHO) to total carbohydrates and NSCHO to structural carbohydrate ratios decreased (p<0.05), while lignin to NSCHO and lipid CH3 symmetric to CH2 symmetric ratios increased with advancing maturity (p<0.05). The FT/IR spectra related to structural carbohydrates, lignin and lipids were distinguished for alfalfa hay at three maturities by PCA and CLA, while FT/IR molecular structures related to carbohydrates and lipids were similar between alfalfa hay cut in the morning and afternoon when analyzed by PCA and CLA analysis. Positive correlations were found for FT/IR NSCHO to total carbohydrate and NSCHO to structural carbohydrate ratios with non-fiber carbohydrate (by wet chemistry), ruminal fast and intermediately degradable carbohydrate fractions and total ruminal degradability of carbohydrates and predicted intestinal nutrient availability in dairy cows ($r{\geq}0.60$; p<0.05) whereas FT/IR lignin to NSCHO and CH3 to CH2 symmetric stretching ratio had negative correlation with predicted ruminal and intestinal nutrient availability of alfalfa hay in dairy cows ($r{\geq}-0.60$; p<0.05). Conclusion: FT/IR carbohydrate and lipid molecular structures in alfalfa hay changed with advancing maturity from early bud to early flower, but not during the day, and these molecular structures correlated with predicted nutrient supply of alfalfa hay in ruminants.

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참고문헌

  1. Yari M, Valizadeh R, Naserian AA, et al. Botanical traits, protein and carbohydrate fractions, ruminal degradability and energy contents of alfalfa hay harvested at three stages of maturity and in the afternoon and morning. Anim Feed Sci Technol 2012;172:162-70. https://doi.org/10.1016/j.anifeedsci.2012.01.004
  2. Yu P, Christensen D, McKinnon J, Markert J. Effect of variety and maturity stage on chemical composition, carbohydrate and protein subfractions, in vitro rumen degradability and energy values of timothy and alfalfa. Can J Anim Sci 2003;83:279-90. https://doi.org/10.4141/A02-053
  3. Burns J, Fisher D, Mayland H. Diurnal shifts in nutritive value of alfalfa harvested as hay and evaluated by animal intake and digestion. Crop Sci 2007;47:2190-7. https://doi.org/10.2135/cropsci2007.02.0072
  4. Yari M, Valizadeh R, Naserian A, et al. Effects of including alfalfa hay cut in the afternoon or morning at three stages of maturity in high concentrate rations on dairy cows performance, diet digestibility and feeding behavior. Anim Feed Sci Technol 2014;92:62-72.
  5. Yu P, Nuez-Ortin WG. Relationship of protein molecular structure to metabolisable proteins in different types of dried distillers grains with solubles: a novel approach. Br J Nutr 2010;104:1429-37. https://doi.org/10.1017/S0007114510002539
  6. Van Soest PJ. Nutritional ecology of the ruminant. Cornell University Press; 1994.
  7. Zhang X, Yu P. Relationship of carbohydrate molecular spectroscopic features in combined feeds to carbohydrate utilization and availability in ruminants. Spectrochim Acta A Mol Biomol Spectrosc 2012;92:225-33. https://doi.org/10.1016/j.saa.2012.01.070
  8. Buta JG, Zadrazil F, Galletti GC. FT-IR determination of lignin degradation in wheat straw by white rot fungus Stropharia rugosoannulata with different oxygen concentrations. J Agric Food Chem 1989;37:1382-4. https://doi.org/10.1021/jf00089a038
  9. Elgersma A, Ellen G, Horst H, et al. Influence of cultivar and cutting date on the fatty acid composition of perennial ryegrass (Lolium perenne L.). Grass Forage Sci. 2003;58:323-31. https://doi.org/10.1046/j.1365-2494.2003.00384.x
  10. Kalac P, Samkova E. The effects of feeding various forages on fatty acid composition of bovine milk fat: A review. Czech J Anim Sci 2010;55:521-37. https://doi.org/10.17221/2485-CJAS
  11. Boufaied H, Chouinard P, Tremblay G, et al. Fatty acids in forages. I. Factors affecting concentrations. Can J Anim Sci 2003;83:501-11. https://doi.org/10.4141/A02-098
  12. Dewhurst RJ, Scollan N, Youell S, Tweed J, Humphreys M. Influence of species, cutting date and cutting interval on the fatty acid composition of grasses. Grass Forage Sci 2001;56:68-74. https://doi.org/10.1046/j.1365-2494.2001.00247.x
  13. Yari M, Valizadeh R, Naserian AA, Jonker A, Yu P. Protein molecular structures in alfalfa hay cut at three stages of maturity and in the afternoon and morning and relationship with nutrient availability in ruminants. J Sci Food Agric 2013;93:3072-80. https://doi.org/10.1002/jsfa.6141
  14. Kalu BA, Fick GW. Quantifying morphological development of alfalfa for studies of herbage quality. Crop Sci 1981;21:267-71. https://doi.org/10.2135/cropsci1981.0011183X002100020016x
  15. Wetzel D, Eilert A, Pietrzak L, Miller S, Sweat J. Ultraspatially-resolved synchrotron infrared microspectroscopy of plant tissue in situ. Cell Mol Biol 1998;44:145-68.
  16. Yu P. Synchrotron-based microspectroscopic analysis of molecular and biopolymer structures using multivariate techniques and advanced multi-components modeling. Can J Anal Sci Spectrosc 2008;53:220-31.
  17. Yu P, Damiran D. Heat-induced changes to lipid molecular structure in Vimy flaxseed: Spectral intensity and molecular clustering. Spectrochim Acta A Mol Biomol Spectrosc 2011;79:51-9. https://doi.org/10.1016/j.saa.2011.01.051
  18. Abeysekara S, Yu P. Response and sensitivity of lipid related molecular structure to wet and dry heating in Canola tissue. Spectrochim Acta A Mol Biomol Spectrosc 2012;90:63-71. https://doi.org/10.1016/j.saa.2011.12.045
  19. Lanzas C, Sniffen C, Seo SA, Tedeschi L, Fox D. A revised CNCPS feed carbohydrate fractionation scheme for formulating rations for ruminants. Anim Feed Sci Technol 2007;136:167-90. https://doi.org/10.1016/j.anifeedsci.2006.08.025
  20. Yari M, Valizadeh R, Naserian AA, Jonker A, Yu P. Modeling nutrient availability of alfalfa hay harvested at three stages of maturity and in the afternoon and morning in dairy cows. Anim Feed Sci Technol 2012;178:12-9. https://doi.org/10.1016/j.anifeedsci.2012.09.001
  21. Tamminga S, Van Straalen W, Subnel A, et al. The Dutch protein evaluation system: the DVE/OEB-system. Livest Prod Sci 1994;40:139-55. https://doi.org/10.1016/0301-6226(94)90043-4
  22. Statistical Analysis System. SAS user's guide: statistics 9.2 ed. SAS Institute; 2003.
  23. Jonker A, Gruber M, Wang Y, et al. Foam stability of leaves from anthocyanidin-accumulating Lc-alfalfa and relation to molecular structures detected by fourier-transformed infrared-vibration spectroscopy. Grass Forage Sci 2012;67:369-81. https://doi.org/10.1111/j.1365-2494.2012.00853.x
  24. Llamas-Lamas G, Combs D. Effect of alfalfa maturity on fiber utilization by high producing dairy cows. J Dairy Sci 1990;73:1069-80. https://doi.org/10.3168/jds.S0022-0302(90)78766-8
  25. Nelson W, Satter L. Effect of stage of maturity and method of preservation of alfalfa on production by lactating dairy cows. J Dairy Sci 1990;73:1800-11. https://doi.org/10.3168/jds.S0022-0302(90)78860-1
  26. Sheaffer CC, Martin NP, Lamb JF, et al. Leaf and stem properties of alfalfa entries. Agron J 2000;92:733-9. https://doi.org/10.2134/agronj2000.924733x
  27. Thompson D, Brooke B, Garland G, Hall J, Majak W. Effect of stage of growth of alfalfa on the incidence of bloat in cattle. Can J Anim Sci 2000;80:725-7. https://doi.org/10.4141/A00-065
  28. Glasser F, Doreau M, Maxin G, Baumont R. Fat and fatty acid content and composition of forages: a meta-analysis. Anim Feed Sci Technol 2013;185:19-34. https://doi.org/10.1016/j.anifeedsci.2013.06.010
  29. Yu P. Short communication: Relationship of carbohydrate molecular spectroscopic features to carbohydrate nutrient profiles in co-products from bioethanol production. J Dairy Sci 2012;95:2091-6. https://doi.org/10.3168/jds.2011-4885
  30. Belanche A, Weisbjerg MR, Allison GG, Newbold CJ, Moorby JM. Measurement of rumen dry matter and neutral detergent fiber degradability of feeds by Fourier-transform infrared spectroscopy. J Dairy Sci 2014;97:2361-75. https://doi.org/10.3168/jds.2013-7491

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