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Structural Analysis and Biological Activities of Sesquiterpene Lactones Isolated from the Leaves and Stems of Chrysanthemum boreale Makino

산국의 잎과 줄기에서 분리한 Sesquiterpene Lactone들의 구조규명 및 생리활성

  • Lee, Jong Rok (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Park, Moon Ki (Department of Pharmaceutical Engineering, Daegu Haany University)
  • 이종록 (대구한의대학교 제약공학과) ;
  • 박문기 (대구한의대학교 제약공학과)
  • Received : 2017.10.24
  • Accepted : 2017.11.27
  • Published : 2017.11.30

Abstract

Chrysanthemum boreale Makino is widely distributed in Korea, China, Japan and Southeast Asian countries. C. boreale is one of the herbs used for treating various inflammatory diseases in oriental medicine. The present study was conducted to identify biologically active compounds from the leaves and stems of C. boreale. We isolated two sesquiterpene sactones from the leaves and stems of C. boreale using silica gel column chromatography and recyclic high perfomance liquid chromatography. The lactones were characterized by their spectroscopic data (NMR, IR, MASS). These compounds were subjected to Farnesyl Protein Transferase (FPTase) inhibition, Nitric Oxide (NO) release inhibition and apoptosis inhibition. The structur of the following isolated compound were elucidated 8,10-${\small{O}$-Acetyl-2-methoxy-10-hydroxy-3,11(13)-guaiadiene-12,6-olide and 4,10-dihydroxy-8-${\small{O}$-Acetyl-2,11(13)-guaiadiene-12,6-olide. In the NO release inhibition assay, compound 2 showed strong activities, with an $IC_{50}$ value of $7{\mu}g/mL$, whereas compound 1 did not exhibit significant activity with an $IC_{50}$ value of over $14{\mu}g/mL$ against murine macrophage.

Acknowledgement

Supported by : 대구한의대학교

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