Journal of Chitin and Chitosan (한국키틴키토산학회지)

The Korean Society for Chitin and Chitosan (한국키틴키토산학회)
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Sulfated Polysaccharide Protects against Ionizing Radiation-induced Skin Damages

이온화 방사선으로 인한 피부 손상에 대한 Sulfated Polysaccharide의 보호 효과

  • Kim, Areum (Interdisciplinary Graduate Program in Advanced Convergence Technology & Science, Jeju National University) ;
  • Cho, Jinhee (College of Veterinary Medicine, Jeju National University) ;
  • Madushani Herath, Kalahe Hewage Iresha Nadeeka (Interdisciplinary Graduate Program in Advanced Convergence Technology & Science, Jeju National University) ;
  • Jee, Youngheun (Interdisciplinary Graduate Program in Advanced Convergence Technology & Science, Jeju National University)
  • 김아름 (제주대학교 차세대융복합과학기술협동과정) ;
  • 조진희 (제주대학교 수의과대학 수의학과) ;
  • ;
  • 지영흔 (제주대학교 차세대융복합과학기술협동과정)
  • Received : 2017.12.08
  • Accepted : 2017.12.18
  • Published : 2017.12.31
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Abstract

Sulfated polysaccharide (SP), generally known as fucoidan and commonly found in the cell walls of brown algae, has been reported to present biological effects including anti-inflammatory, anticancer, and antioxidant activities. Exposure to ionizing gamma-ray induces cellular production of reactive oxygen species and hair follicular cells, being the most sensitive to gammaray immediately go through apoptosis and damage their rapid differentiation essential for regeneration of hair. In this study we investigated the protective potential of SP's against gamma-ray induced hair follicle cells injury. For that, 8.5Gy gamma-ray was irradiated to the whole body of C57BL/6 mouse at day 6 after depilation treated with and without SP. Treatment with SP markedly inhibited formation of thiobarbituric acid-reactive substances(TBARS) compared to control group (without SP) after 48 h of irradiation. Increased cleaved caspase-3 and p53 expression by irradiation was decreased by treatment with SP after 8 h of irradiation. Furthermore, terminal deoxyribonucleotidyl transferase-mediated d-uridine 5' triphosphate nick end labelling (TUNEL) staining showed that the proportion of apoptotic cells in gamma-ray irradiated skins was significantly lower in mouse treated with SP than control group (without SP). Also, SP treatment increased the percentage of Ki-67 positive cells compared with those of irradiation only group. These results suggest that SP has potential as a radioprotector for hair follicular cells.

본 연구에서는 F. vesiculosus에서 fucoidan을 이용하여 피부에 대한 방사선 보호 효과를 확인하기 위한 실험을 수행하였다. 방사선을 조사한 마우스피부의 지질과산화물 함량이 방사선을 조사하지 않은 건강한 마우스 피부 조직보다 유의적으로 증가하였고, SP를 도포하였을 경우 지질과산화물의 함량이 유의적으로 감소하는 것을 확인하였다. 또한 SP의 도포로 인하여 cleaved caspase-3와 p53의 발현이 감소함으로써 세포자멸사(apoptosis)가 억제되었다. 세포 증식능 표지자로 세포주기(cell cycle) 중 M, G1, S, G2기에 발현하며, 세포분열기인 M기에 특히 많이 발현한다고 알려진 Ki-67의 발현이 증가함으로써 방사선으로 인하여 손상된 모낭세포의 복구를 증진시켰다. 이상의 결과로부터, SP의 도포는 방사선으로 인한 과산화물의함량을감소시켜모낭세포의세포자멸사(apoptosis)를 억제시키고, 또한 방사선으로 인하여 손상된 모낭세포의 복구를 증진시킴으로써 방사선에 대한 보호 효과를 가진다는 것을 확인하였다. 이는 SP가 방사선에 의한 모낭세포 손상에 대한 보호제로서의 가능성을 시사한다.

Keywords

Acknowledgement

Supported by : 제주대학교

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