The Journal of the Korean dental association (대한치과의사협회지)

The Korean Dental Association (대한치과의사협회)
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Application of the QLF technology to monitor recovery rates of enamel caries lesions with human saliva

법랑질 병소 회복율 평가를 위한 QLF 기술의 적용

  • Kim, Gyung-Min (Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry) ;
  • Ku, Hye-Min (Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry) ;
  • Lee, Eun-Song (Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry) ;
  • Kang, Si-Mook (Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry) ;
  • Jong, Elbert de Josselin de (Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry) ;
  • Kwon, Ho-Keun (Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry) ;
  • Kim, Baek-Il (Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry)
  • 김경민 (연세대학교 치과대학 예방치과학교실) ;
  • 구혜민 (연세대학교 치과대학 예방치과학교실) ;
  • 이은송 (연세대학교 치과대학 예방치과학교실) ;
  • 강시묵 (연세대학교 치과대학 예방치과학교실) ;
  • ;
  • 권호근 (연세대학교 치과대학 예방치과학교실) ;
  • 김백일 (연세대학교 치과대학 예방치과학교실)
  • Received : 2016.12.06
  • Accepted : 2017.01.03
  • Published : 2017.02.28
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Abstract

Purpose: The aim of this in vitro study was to assess changes in remineralization by stimulated human saliva over a short period of 48 hours with quantitative light-induced fluorescence (QLF) technology. Materials and Methods: Bovine incisor surfaces were demineralized for 10 days. Two types of stimulated saliva were collected from 7 healthy persons. 24 hours after tooth brushing (Stimulated saliva group) and immediately after tooth brushing with 1,000 ppm NaF dentifrice (Dentifrice saliva group). The specimens were immersed in saliva and fluorescence images were obtained by QLF-digital (QLF-D $biluminator^{TM}$,) at 2, 4, 6, 12, 24, and 48 hours fluorescence loss (${\Delta}F%$) of the lesions. A paired t-test was performed to assess fluorescence differences between before (${\Delta}F_{baseline}$) and after (${\Delta}F_{treatment\;time}$) the remineralization process. Results: Before the remineralization, the mean ${\Delta}F_{baseline}$ of the initial demineralized specimens was $-18.42{\pm}0.15$ (%). In both groups, the ${\Delta}F$ values obtained at baseline and after 2 hours were statistically significant (P < 0.001), indicating recovery of the lesions by approximately 40% after 2 hours. After 48 hours, remineralization rates were slightly higher (49%) for the stimulated saliva group than for the dentifrice saliva group (41%), but the difference was not statistically significant. Conclusions: With QLF minute degrees of remineralization by saliva can be measured in periods as short as 2 hours. Additionally no significantly higher effects of remineralization were observed in the dentifrice saliva group when compared to the stimulated saliva group.

Keywords

References

  1. Oliveira GM, Ritter AV, Heymann HO, et al. Remineralization effect of CPP-ACP and fluoride for white spot lesions in vitro. J Dent 2014;42(12):1592-1602. https://doi.org/10.1016/j.jdent.2014.09.004
  2. Hannig M, Balz M. Influence of in vivo formed salivary pellicle on enamel erosion. Caries Res 1999;33(5):372-379. https://doi.org/10.1159/000016536
  3. Brevik S, Lussi A, Rakhmatullina E. A new optical detection method to assess the erosion inhibition by in vitro salivary pellicle layer. J Dent 2013;41(5):428-435. https://doi.org/10.1016/j.jdent.2013.02.011
  4. Ionta FQ, Mendon?a FL, de Oliveira GC, et al. In vitro assessment of artificial saliva formulations on initial enamel erosion remineralization. J Dent 2014;42(2):175-179. https://doi.org/10.1016/j.jdent.2013.11.009
  5. Meller C, Heyduck C, Tranaeus S, Splieth C. A new in vivo method for measuring caries activity using quantitative light-induced fluorescence. Caries Res 2006;40(2):90-96. https://doi.org/10.1159/000091053
  6. Meller C, Santamaria R, Connert T, Splieth C. Predicting caries by measuring its activity using quantitative light-induced fluorescence in vivo: a 2-year caries increment analysis. Caries Res 2012;46(4):361-367. https://doi.org/10.1159/000338379
  7. Cochrane NJ, Walker GD, Manton DJ, Reynolds EC. Comparison of quantitative light-induced fluorescence, digital photography and transverse microradiography for quantification of enamel remineralization. Aust Dent J 2012;57(3):271-276. https://doi.org/10.1111/j.1834-7819.2012.01706.x
  8. Restrepo M, Bussaneli DG, Jeremias F, et al. Control of white spot lesion adjacent to orthodontic bracket with use of fluoride varnish or chlorhexidine gel. The Scientific World Journal 2015;2015:218452.
  9. Gmur R, Giertsen E, van der Veen MH, et al. In vitro quantitative light-induced fluorescence to measure changes in enamel mineralization. Clin Oral Investig 2006;10(3):187-195. https://doi.org/10.1007/s00784-006-0058-z
  10. Angmar-Mansson B, ten Bosch JJ. Quantitative light-induced fluorescence (QLF): a method for assessment of incipient caries lesions. Dentomaxillofac Radiol 2001;30(6):298-307. https://doi.org/10.1038/sj.dmfr.4600644
  11. 김백일. QLF 의 원리와 임상적 활용. 대한치과의사협회지 2011;49(8):443-450.
  12. Fujikawa H, Matsuyama K, Uchiyama A, et al. Influence of salivary macromolecules and fluoride on enamel lesion remineralization in vitro. Caries Res 2008;42(1):37-45. https://doi.org/10.1159/000111748
  13. al-Khateeb S, ten Cate JM, Angmar-Mansson B, et al. Quantification of formation and remineralization of artificial enamel lesions with a new portable fluorescence device. Adv Dent Res 1997;11(4):502-506. https://doi.org/10.1177/08959374970110041801
  14. Kim HE, Kwon HK, Kim B. Recovery percentage of remineralization according to severity of early caries. Am J Dent 2013;26(3):132-136.
  15. Pretty I, Pender N, Edgar W, Higham S. The in vitro detection of early enamel de?and re?mineralization adjacent to bonded orthodontic cleats using quantitative light-induced fluorescence. Eur J Orthod 2003;25(3):217-223. https://doi.org/10.1093/ejo/25.3.217
  16. Lussi A, von Salis-Marincek M, Ganss C, et al. Clinical study monitoring the pH on tooth surfaces in patients with and without erosion. Caries Res 2012;46(6):507-512. https://doi.org/10.1159/000339783
  17. Hara A, Ando M, Gonzalez-Cabezas C, et al. Protective effect of the dental pellicle against erosive challenges in situ. J Dent Res 2006;85(7):612-616 https://doi.org/10.1177/154405910608500706
  18. Eisenburger M, Addy M, Hughes J, Shellis R. Effect of time on the remineralisation of enamel by synthetic saliva after citric acid erosion. Caries Res 2001;35(3):211-215. https://doi.org/10.1159/000047458
  19. Ten Cate J. Remineralization of caries lesions extending into dentin. J Dent Res 2001;80(5):1407-1411. https://doi.org/10.1177/00220345010800050401
  20. Vogel G, Mao Y, Chow L, Proskin H. Fluoride in plaque fluid, plaque, and saliva measured for 2 hours after a sodium fluoride monofluorophosphate rinse. Caries Res 2000;34(5):404-411. https://doi.org/10.1159/000016615
  21. Duckworth R, Jones Y, Nicholson J, et al. Studies on plaque fluoride after use of F-containing dentifrices. Adv Dent Res 1994;8(2):202-207. https://doi.org/10.1177/08959374940080021101
  22. Garcia-Godoy F, Hicks MJ. Maintaining the integrity of the enamel surface: the role of dental biofilm, saliva and preventive agents in enamel demineralization and remineralization. J Am Dent Assoc. 2008;139:25S-34S. https://doi.org/10.14219/jada.archive.2008.0352
  23. Featherstone JD. The science and practice of caries prevention. J Am Dent Assoc. 2000;131(7):887-899. https://doi.org/10.14219/jada.archive.2000.0307