Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant activities and anti-inflammatory effects of fresh and air-dried Abeliophyllum distichum Nakai leaves

건조방법에 따른 미선나무 잎의 항산화 및 항염증 효과

  • Chang, Seong Jun (Teakyung food and processing R&D center) ;
  • Jeon, Nam Bae (Teakyung food and processing R&D center) ;
  • Park, Joo Won (Teakyung food and processing R&D center) ;
  • Jang, Tae Won (Department of Medicinal Plant Resources, Andong National University) ;
  • Jeong, Jin Boo (Department of Medicinal Plant Resources, Andong National University) ;
  • Park, Jae Ho (Department of Medicinal Plant Science, Jungwon University)
  • Received : 2017.12.06
  • Accepted : 2018.01.26
  • Published : 2018.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we evaluated the antioxidant activity and anti-inflammatory effects of Abeliophyllum distichum (A. distichum) leaves that were prepared via air-drying. Fresh and air-dried A. distichum leaves were examined via 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay and measurements of the reducing power. The suppression effects on inflammation of the leaves were analyzed by a western blot and RT-PCR on LPS-induced RAW 264.7 cells. As a result, the antioxidant activity of the fresh leaves was found to be more effective than that of the air-dried leaves. Also, the fresh leaves were more effective in suppressing the protein and mRNA levels of iNOS and COX-2 than the air-dried leaves, thereby indicating the better anti-inflammatory effects. In addition, the contents of phenolic compounds and acteoside were analyzed by high-performance liquid chromatography (HPLC). The results showed that the acteoside content decreased with the use of the air-drying method, while there was no change in the content of phenolic compounds. Therefore, this study indicated that fresh A. distichum leaves potential antioxidant and suppression activities of various factors that are involved in the production of NO, which were found to be better than those of air-dried A. distichum leaves. These biological activities were also found to be independent of the content of phonolic compounds and were assumed to be directly or indirectly related to the content of acteoside.

건조 방식에 따른 미선나무 잎의 항산화 활성 및 항염증 효과를 확인한 결과, 높은 항산화 활성를 나타냈으며, 건조 잎에 비해 생잎의 항산화 활성이 뛰어났다. 페놀류 화합물의 함량은 유의적인 차이가 없었으나, 미선나무의 활성물질로 알려진 acteoside의 함량을 분석한 결과, 생잎의 acteoside의 함량이 높았다. 또한 항염증 효과를 확인하기 위해 nitric oxide의 함량 및 세포 수준에서의 염증 관련 인자인 iNOS와 COX-2의 단백질 발현 및 mRNA 수준을 확인한 결과, 생잎의 억제활성이 건조잎보다 높았다. 이를 통해 건조 잎에 비해 생잎의 이용가치가 높았으며, 이러한 항산화 및 항염증 활성은 acteoside의 함량의 차이와 밀접한 관련이 있는 것으로 사료된다.

Keywords

References

  1. Maxwell SR (1995) Prospects for the use of antioxidant therapies. Drugs, 49, 345-361
  2. Asensi M, Ortega A, Mena S, Feddi F, Estrela JM (2011) Natural polyphenols in cancer therapy. Crit Rev Clin Lab Sci, 48, 197-216
  3. Kehrer JP (1993) Free radicals as mediators of tissue injury and disease. Crit Rev Toxicol, 23, 21-48
  4. Lawrence T, Willoughby DA, Gilroy DW (2002) Anti-inflammatory lipid mediators and insights into the resolution of inflammation. Nat Rev Immunol, 2, 787-795
  5. Choi DS (2009) Study on the antiinflammatory effect and mechanism of isoegomaketone in vitro and in vivo. MS Thesis, Chonbuk National University, Korea, p 4-5
  6. Chae CH (2004) Functional role of inducible nitric oxide synthase and cyclooxygenase-2 in acute inflammation. Ministry of Health and Welfare, Gwacheon, Korea, p 10-11
  7. Bak JP, Son JH, Kim YM, Jung JH, Leem KH, Lee EY, Kim EH (2011) Suppression effect of the inflammatory response in macrophages by paeoniae radix rubra extracts. Korean J Med Crop Sci, 19, 373-379
  8. Jung HK, Kang BM, Jang JH, Ahn BK, Yeo JH, Jung WS, Cho JH, Kuk YI, Hyun KH, Cho HW (2014) Inhibitory effect of Alopecurus aequalis Sobol ethanol extracts on LPS-induced inflammatory response in RAW 264.7 cells. Korean J Med Crop Sci, 22, 98-104
  9. Park JH (2011) Antioxidant activities and inhibitory effect on oxidative DNA damage of extracts from abeliophylli distichi folium. Korean J Herbol, 26, 95-99
  10. Oh H, Kang DG, Kwon TO, Jang KK, Chai KY, Yun YG, Chung HT, Lee HS (2003) Four glycosides from the leaves of Abeliophyllum distichum with inhibitory effects on angiotensin converting enzyme. Phytother Res, 17, 811-813
  11. Park GH, Park JH, Eo HJ, Song HM, Lee MH, Lee JR, Jeong JB (2014) Anti-inflammatory effect of the extracts from Abeliophyllum distichum Nakai in LPS-stimulated RAW264.7 cells. Korean J Plant Res, 27, 209-214
  12. Xiong Q, Hase K, Tezuka Y, Tani T, Namba T, Kadota S (1998) Hepatoprotective activity of phenylethanoids from Cistanche deserticola. Planta Med, 64, 120-125
  13. Schlesier K, Harwat M, Bohm V, Bitsch R (2002) Assessment of antioxidant activity by using different in vitro methods. Free Radic Res, 36, 177-187
  14. He ZD, Lau KM, Xu HX, Li PC, Pui-Hay BP (2000) Antioxidant activity of phenylethanoid glycosides from Brandisia hancei. J Ethnopharmacol, 71, 483-486
  15. Li J, Wang PF, Zheng R, Liu ZM, Jia Z (1993) Protection of phenylpropanoid glycosides from pedicularis against oxidative hemolysis in vitro. Planta Med, 59, 315-317
  16. Xiong Q, Kadota S, Tani T, Namba T (1996) Antioxidative effects of phenylethanoids from Cistanche deserticola. Biol Pharm Bull, 19, 1580-1585
  17. Bondet V, Brand-Williams W, Berset C (1997) Kinetics and mechanisms of antioxidant activity using the DPPH free radical method. LWT-Food Sci Technol, 30, 609-615
  18. Van den Berg R, Haenen GR, Van den Berg H, Bast A (1999) Applicability of an improved trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures. Food Chem, 66, 511-517
  19. Oyaizu M (1986) Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr, 44, 307-315
  20. AOAC (1995) Official Methods of Analysis. 14th ed, Association of Official Analytical Chemists, Washington DC, USA, p 8-35
  21. Que F, Mao L, Pan X (2006) Antioxidant activities of five Chinese rice wines and the involvement of phenolic compounds. Food Res Int, 39, 581-587
  22. Ferreira IC, Baptista P, Vilas-Boas M, Barros L (2007) Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food Chem, 100, 1511-1516
  23. Ferreres F, Gomes D, Valentano P, Goncalves R, Pio R, Chagas EA, Seabra RM, Andrade PB (2009) Improved loquat (Eriobotrya japonica Lindl.) cultivars: Variation of phenolics and antioxidative potential. Food Chem, 114, 1109-1127
  24. Bremer B, Bremer K, Heidari N, Erixon P, Olmstead RG, Anderberg AA, Kallersjo M, Barkhordarian E (2002) Phylogenetics of asterids based on 3 coding and 3 non-coding chloroplast DNA markers and the utility of non-coding DNA at higher taxonomic levels. Mol Phylogenet Evol, 24, 274-301
  25. Szabo C (1998) Role of nitric oxide in endotoxic shock: An overview of recent advances. Ann N Y Acad Sci, 851, 422-425
  26. Martel-Pelletier J, Pelletier JP, Fahmi H (2003) Cyclooxygenase-2 and prostaglandins in articular tissues. Semin Arthritis Rheum, 33, 155-167
  27. Weisz A, Cicatiello L, Esumi H (1996) Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferongamma, bacterial lipopolysaccharide and NG-monomethyl-L-arginine. Biochem J, 316, 209-215
  28. An SM, Kim HG, Choi EJ, Hwang HH, Lee ES, Baek JH, Boo YC, Koh JS (2014) Screening for anti-inflammatory activities in extracts from Korean herb medicines. J Soc Cosmet Sci Korea, 40, 95-108
  29. Kang CH, Choi YH, Choi IW, Lee JD, Kim GY (2011) Inhibition of lipopolysaccharide-induced iNOS, COX-2, and TNF-$\alpha$ expression by aqueous extract of Orixa japonica in Raw 264.7 cells via suppression of NF-${\kappa}B$ activity. Trop J Pharm Res, 10, 161-168
  30. Hur GM, Ryu YS, Yun HY, Jeon BH, Kim YM, Seok JH, Lee JH (1999) Hepatic ischemia/reperfusion in rats induces iNOS gene transcription by activation of NF-kappaB. Biochem Biophys Res Commun, 261, 917-922
  31. Sato T, Nakajima H, Fujio K, Mori Y (1997) Enhancement of prostaglandin E2 production by epidermal growth factor requires the coordinate activation of cytosolic phospholipase A2 and cyclooxygenase 2 in human squamous carcinoma A431 cells. Prostaglandins, 53, 355-369
  32. Huang M, Stolina M, Sharma S, Mao JT, Zhu L, Miller PW, Wollman J, Herschman H, Dubinett SM (1998) Non-small cell lung cancer cyclooxygenase-2-dependent regulation of cytokine balance in lymphocytes and macrophages: up-regulation of interleukin 10 and down-regulation of interleukin 12 production. Cancer Res, 58, 1208-1216

Cited by

  1. Sprague-Dawley 랫드를 이용한 미선나무주정추출물의 2주 반복 경구투여 독성평가 vol.34, pp.6, 2018, https://doi.org/10.7318/kjfc/2019.34.6.801
  2. Prediction and Identification of Biochemical Pathway of Acteoside from Whole Genome Sequences of Abeliophyllum Distichum Nakai, Cultivar Ok Hwang 1ho vol.10, pp.3, 2018, https://doi.org/10.22156/cs4smb.2020.10.03.076
  3. 섬기린초(Sedum takesimense)에서 분리된 화합물들의 RAW 264.7 Cell에 대한 항염증 효과와 여드름 원인균에 대한 항균 효과 vol.54, pp.4, 2021, https://doi.org/10.5657/kfas.2021.0552