Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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The anti-oxidant, whitening and anti-wrinkle effects of Castanea crenata inner shell extracts processed by enzyme treatment and pressurized extraction

효소처리 및 가압추출 공정을 이용한 율피 추출물의 항산화, 피부 미백 및 주름개선 효과

  • Gu, Yul Ri (Department of Food Science and Technology, Daegu Catholic University) ;
  • Kim, Ju Hyeon (Department of Food Science and Technology, Daegu Catholic University) ;
  • Hong, Joo-Heon (Department of Food Science and Technology, Daegu Catholic University)
  • 구율리 (대구가톨릭대학교 식품공학전공) ;
  • 김주현 (대구가톨릭대학교 식품공학전공) ;
  • 홍주헌 (대구가톨릭대학교 식품공학전공)
  • Received : 2017.12.06
  • Accepted : 2018.01.26
  • Published : 2018.02.28
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Abstract

In this study, the anti-oxidant, whitening, and anti-wrinkle effects of Castanea crenata inner shell extracts processed by enzyme treatment and pressurized extraction were investigated. The Castanea crenata inner shell was first hydrolyzed using celluclast, viscozyme, or hemicellulase. Then, it was subjected to pressure extraction for different durations (30, 60, and 120 min). The yields of the Castanea crenata inner shell extracts processed by different enzyme treatments followed by pressurized extraction for different times are in the range of 12.42-29.80%. The total polyphenol, flavonoid, and tannin contents of the C30m (celluclast enzyme and autoclave extracts at 30 min) extract were 15.48, 10.82, and 15.82 g/100 g, respectively. The total sugar content of the H120m(hemicellulase enzyme and autoclave extracts at 120 min) extract is 61.07 g/100 g. The 1,1-diphenyl-2-pycrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities of the C30m extract at $1,000{\mu}g/mL$ are 89.20, and 81.96%, respectively. The superoxide radical scavenging and ferric-reducing antioxidant power of the C30m extract at $1,000{\mu}g/mL$ are 67.63% and $1,324.79{\mu}M$, respectively. Further, the tyrosinase and elastase inhibition activity of the C30m extract at $1,000{\mu}g/mL$ are 61.32, and 61.06%, respectively. Our results indicate that the Castanea crenata inner shell extracts processed by enzyme treatment followed by pressurized extraction could have beneficial effects on facial skin and they should be considered for use in new functional cosmetics.

본 연구에서는 율피 추출물을 이용하여 화장품 소재로서의 기능성을 조사하고자 효소종류별 및 가압추출 시간별에 따른 항산화 활성과 피부 미백 및 주름개선 효과를 실시하였다. 효소 종류별 및 가압추출 시간에 따른 율피 추출물의 추출수율은 12.42-29.80%로, C30m 추출물이 29.80%로 가장 높게 나타났다. 이화학적 특성으로 총 폴리페놀, 총 플라보노이드 및 총 탄닌 함량은 모두 C30m 추출물($1,000{\mu}g/mL$)에서 각각 22.69 g/100 g, 12.13 g/100 g 및 21.66 g/100 g으로 높은 함량을 나타내었으며, 총 당 함량은 H120m 추출물($1,000{\mu}g/mL$)에서 61.07 g/100 g으로 높은 함량을 나타내었다. 항산화 활성으로 DPPH radical 소거활성, ABTS radical 소거활성, superoxide radical 소거활성 및 FRAP 활성 모두 C30m 추출물($1,000{\mu}g/mL$)에서 각각 89.20%, 81.96%, 67.63% 및 $1,324.79{\mu}M$로 높은 항산화 활성을 나타내었으며, 모든 효소 처리구간에서 가압추출 시간이 증가함에 따라 항산화 활성이 감소하였다. 피부 미백 및 주름개선 효과를 확인하기 위해 tyrosinase 및 elastase 저해활성을 측정한 결과 C30m 추출물($1,000{\mu}g/mL$)에서 각각 61.32% 및 61.06%로 높은 저해활성을 나타내었으며, HE 추출물에서 가장 낮은 저해활성을 나타내었다. 이는 효소처리 및 가압추출에 의해 율피의 생리활성물질이 많이 용출된 것으로 판단되며, 율피의 새로운 추출공정 확립을 통해 항산화, 피부 미백 및 주름개선에 효과가 있는 항노화 기능성 소재로 화장품 제조용 원료 로 적합함을 확인하였다.

Keywords

References

  1. Fisher GJ, Talwar HS, Lin J, Voorhees JJ (1999) Molecular mechanisms of photoaging in human skin in vivo and their prevention by all-trans retinoic acid. Photochem Photobiol, 69, 154-157
  2. Halliwell B, Aeschbach R, Lliger J, Aruoma OI (1995) The characterization of antioxidants. Food Chem Toxicol, 33, 601-617
  3. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol, 39, 44-84
  4. Wang KH, Lin RD, Hsu FL, Huang YH, Chang HC, Huang CY, Lee MH (2006) Cosmetic applications of selected traditional chinese herbal medicines. J Ethnopharmacol, 106, 353-359
  5. Brenneisen P, Sies H, Scharffetter-Lochanek K (2002) Ultraviolet-B irradiation and matrix metalloproteinase: from induction via signaling to initial events. Ann N Y Acad Sci, 973, 31-43
  6. Philips N, Keller T, Hendrix C, Hamilton S, Arena R, Tuason M, Gonzalez S (2007) Regulation of the extracellular matrix remodeling by lutein in dermal fibroblasts, melanoma cells, and ultraviolet radiation exposed fibroblasts. Arch Dermatol Res, 299, 373-379
  7. Tsukahara K, Nakagawa H, Moriwaki S, Takema Y, Fujimura T, Imokawa G (2006) Inhibition of ultraviolet-B-induced wrinkle formation by an elastase-inhibiting herbal extract: implication for the mechanism underlying elastase-associated wrinkles. Int J Dermatol, 45, 460-468
  8. Joo SY (2013) Antioxidant activities of medicinal plant extracts. J Korean Soc Food Sci Nutr, 42, 512-519
  9. Kang MC, Lee JY, Ko RK, Kim HB, Hong SH, Kim GO (2008) Melanin inhibitory effect and anti-inflammatory effects of Dictyota corlacca extracts derived from adjacent sea of the Jeju island. Korean J biotechnol Bioeng, 23, 311-316
  10. Jung SW, Lee NK, Kim SJ, Han D (1995) Screening of tyrosinase inhibitor from plants. Korean J Food Sci Technol, 27, 891-896
  11. Yagi A, Kanbara T, Morinobu N (1986) The effect of tyrosinase inhibition for aloe. Planta Medica, 3981, 517-519
  12. Lee HJ, Chung MJ, Cho JY, Ham SS, Choe M (2008) Antioxidative and macrophage phagocytic activities and functional component analyses of selected Korean chestnut (Castanea crenata S. et Z) cultivars. J Korean Soc Food Sci Nutr, 37, 1095-1100
  13. Jang MJ, Cheon SJ, Kim HY, Kwoen DJ, Kim HY, Kim SH, Lee JT (2011) The anti-wrinkle and whitening effect of extracts of Castanea crenata inner shell. J Life Sci, 21, 734-738
  14. Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic, 16, 144-158
  15. Jia Z, Tang M, Wu J (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem, 64, 555-559
  16. Duval B, Shetty K (2001) The stimulation of phenolics and antioxidant activity in pea (Pisum sativum) elicited by genetically transformed anise root extract. J Food Biochem, 25, 361-377
  17. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. J Anal Chem, 28, 350-356
  18. Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1200
  19. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med, 26, 1231-1237
  20. Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem, 239, 70-76
  21. Oyaizu M (1986) Studies on products of browning reaction:antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr Diet, 44, 307-315
  22. James AEK, Timothy DW, Gordon L (1996) Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic tyrosin inhibitor. Biochemistry, 35, 9090-9096
  23. Shahidi F, Janitha PK, Wanasundara PD (2009) Phenolic antioxidant. Crit Rev Food Sci Nutr, 32, 67-103
  24. Choi MO, Kim BJ, Jo SK, Jung HK, Lee JT, Kim HY, Kweon DJ (2013) Anti-allergic activities of Castanea crenta inner shell extracts fermented by Lactobacillus bifermentans. Korean J Food Preserv, 20, 583-591
  25. Jo HE, Chong MS, Choi YH (2010) Evaluation of composition and antioxidant activity form Zizyphus jujuba fruits and leaves extracts for development medicinal food. Korean J orient Physiol Pathol, 24, 859-865
  26. Hwang IG, Woo KS, Kim TM, Kim DJ, Yang MH, Jeong HS (2006) Change of physicochemical characteristics of Korean pear (Pyrus pyrifolia Nakai) juice with heat treatment conditions. Korean J Food Sci Technol, 38, 342-347
  27. Shouqin Z, Junjie Z, Changzhen W (2004) Novel high pressure extraction technology. Int J Pharm, 278, 471-474
  28. Koo SY, Cha KH, Lee DU (2007) Effects of high hydrostatic pressure of foods and biological system. Food Science and Industry, 40, 23-30
  29. Tobin D, Thody AJ (1994) The superoside anion may mediate short-but not long-term effects of ultraviolet radiation on melanogenesis. Exp Dermatol, 3, 99-105
  30. Bernadette E, Marianne P, Berngard P (1998) Protective effect against sunburn of combined systemic ascorbic acid (vitamin C) and d-$\alpha$-tocopherol (vitamin E). J Am Acad Dermatol, 38, 45-48
  31. Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR (2004) Screening of the antioxidant activity of some medicinal plants. Korean J Food Sci Technol, 36, 333-338
  32. Yoo KH, Jeong JM (2009) Antioxidative and antiallergic effect of persimmon leaf extracts. J Korean Soc Food Sci Nutr, 38, 1691-1698
  33. Kwon OC, Woo KS, Kim TM, Kim DJ, Hong JT, Jeong HS (2006) Physicochemical characteristics of garlic (Allium sativum L.) on the high temperature and pressure treatment. Korean J Food Sci Technol, 38, 331-336
  34. Sim GS, Kim JH, Lee BC, Lee DH, Lee GS, Pyo HB (2008) Inhibitory effects on melanin production in B16 melanoma cells of Sedum sarmentosum. Yakhak Hoeji, 52, 165-171
  35. Kim SJ, Heo MY, Bae KH, Kang SS, Kim HP (2003) Tyrosinase inhibitory activity of plant extract (III): Fifty Korean indigenous plants. J Appl Phamacol, 11, 245-248
  36. Kim YJ, Uyama H (2005) Tyrosinase inhibitors from natural and synthetic sources: Structure, inhibition mechanism and perspective for the future. Cell Mol Life Sci, 62, 1707-1723
  37. Boissy RE, Manga P (2004) On the etiology of contact/occupational vitiligo. Pigm Cell Res, 17, 208-214
  38. Yang MJ, Lim SJ, Ahn HS, Kim MA, Ahn RM (1999) Inhibitory effects of chestnut bark extracts on tyrosinase activity and melanin biosynthesis. Korean J Environ Health Soc, 25, 37-43
  39. Yang MJ, Kim MG, Lim SJ, Ahn HS, Ahn RM (1999) Inhibitory effects of water-acetone extract of chestnut inner shell, pine needle and hop on the melanin biosynthesis. Yakhak Hoeji, 43, 494-501
  40. Dewitt DL, Rollins TE, Day JS, Gauger JA, Smith WL (1981) Orientation of the active site, and antigenic determinants of prostaglandin endoperoxide of synthase in the endoplasmic reticulum. J Biol Chem, 256, 10375-10382
  41. Kim MK, Kim JY, Choi SW, Hong JT, Yoon KS (2004) Anti-wrinkle effect of safflower (Cathamus tinctorius L.) seed extract. J Soc Cosmet Sci Korea, 30, 15-22
  42. Jeong HR, Kim JH, Jo YN, Jeong JH, Heo HJ (2011) Characterization as cosmetic substances of chestnut inner skim extract with antioxidant activity. J Agric Life Sci, 45, 183-191
  43. Kwak YJ, Lee DH, Kim NM, Lee JS (2005) Screening and extraction condition of anti-skin aging elastase inhibitor from medicinal plants. Korean J Medicianl Crop Sci, 13, 213-216