Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Characteristics of Eggshell Powder as Carriers of Probiotics

생균제의 부형제(운반체)로서의 난각분말의 특성

  • Lee, Woo-Do (Department of Animal Science and Technology, Konkuk University) ;
  • Niu, Kai-Min (Department of Animal Science and Technology, Konkuk University) ;
  • Lim, Jeong-Min (Department of Animal Science and Technology, Konkuk University) ;
  • Yi, Kwon-Jung (Department of Animal Science and Technology, Konkuk University) ;
  • Lee, Bong-Joo (Aquafeed Research Center, National Institute of Fisheries Science) ;
  • Kim, Kang-Woong (Aquafeed Research Center, National Institute of Fisheries Science) ;
  • Kim, Kyoung-Duck (Aquafeed Research Center, National Institute of Fisheries Science) ;
  • Hur, Sang-Woo (Aquafeed Research Center, National Institute of Fisheries Science) ;
  • Han, Hyon-Sob (Aquafeed Research Center, National Institute of Fisheries Science) ;
  • Kim, Soo-Ki (Department of Animal Science and Technology, Konkuk University)
  • 이우도 (건국대학교 동물자원과학과) ;
  • 우개민 (건국대학교 동물자원과학과) ;
  • 임정민 (건국대학교 동물자원과학과) ;
  • 이권정 (건국대학교 동물자원과학과) ;
  • 이봉주 (국립수산과학원 사료연구센터) ;
  • 김강웅 (국립수산과학원 사료연구센터) ;
  • 김경덕 (국립수산과학원 사료연구센터) ;
  • 허상우 (국립수산과학원 사료연구센터) ;
  • 한현섭 (국립수산과학원 사료연구센터) ;
  • 김수기 (건국대학교 동물자원과학과)
  • Received : 2018.01.08
  • Accepted : 2018.01.16
  • Published : 2018.01.30
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Abstract

Eggshell (ES) is a by-product of table eggs with high content of calcium carbonate which can be used as a calcium source in feed. In this study, we have first illuminated the potential application of ES as a novel carrier for probiotics. The carriers used in the study include a SBM (Soybean meal), ESL (Eggshell powder with large particles), ESF (Eggshell powder with fine particles), and the complex carriers (SBM+ESL, SBM+ESF). The structure of carriers absorbed by L. plantarum was confirmed by SEM image. Among these carriers, the complex carrier SBM+ESF showed the highest viability of L. plantarum with pH 7~8 during four weeks storage at room temperature. The SBM+ESF was further tested as a carrier for various probiotic strains at $4^{\circ}C$ or $30^{\circ}C$. All the probiotic strains showed high viability at $4^{\circ}C$ storage. However, a significant reduction of Lactobacillus cells was observed at $30^{\circ}C$ storage. B. lichenifomis maintained high viability whereas B. subtilis, B. amyloliquefaciens, and S. cerevisiae showed the reduction of $2{\log}_{10}$ (CFU/g). These results suggest that if the ESF as a calcium source in feed was mixed with SBM, it can be used as an effective complex carrier for improving the viability of some probiotics including B. licheniformis.

계란 가공 부산물인 난각(ES: Eggshell)은 탄산칼슘 함량이 높아 사료에 첨가하여 칼슘원으로 이용되고 있다. 본 연구에서는 ES를 생균제의 부형제인 운반체로서 활용 가능성을 처음으로 시도하였다. L. plantarum을 대두박(SBM: Soybean meal), 난각조각(ESL: Eggshell powder with large particles), 난각미세분말(ESF: Eggshell powder with fine particles), 그리고 이들의 복합운반체인 SBM+ESL과 SBM+ESF에 생균제를 흡착시켜 그 부착상태를 주사전자현미경으로 확인하였다. 이 중 복합운반체인 SBM+ESF는 상온에서 4주 동안 pH 7~8을 유지하면서 L. plantarum의 가장 높은 생존율을 보였다. 본 연구에 사용한 모든 생균제들은 보존기간 동안 $4^{\circ}C$에서는 높은 생존율을 보였다. $30^{\circ}C$에서는 유산균수는 크게 감소하였으나, B. licheniformis는 높은 생존율을 유지하였고 B. subtilis, B. amyloliquefaciens와 S. cerevisiae는 $2{\log}_{10}$ (CFU/g)정도 감소하였다. 상기 연구결과는 사료의 칼슘원으로 이용되는 난각미세분말(ESF)을 대두박과 혼합하여 사용하면 B. licheniformis를 비롯한 일부 생균제의 생존성을 향상시켜 부형제(운반체)로도 사용할 수 있음을 밝혔다.

Keywords

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