The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory Effects of Ethanol Extract of Chinese Medicinal Plants in Yanjin on LPS-stimulated RAW 264.7 Macrophages

LPS로 자극한 RAW 264.7 세포에서 중국 연변에 자생하는 약용 식물 에탄올 추출물의 항염증 효과 연구

  • Park, Yea-Jin (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Seo, Jong-Hwan (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Gil, Tae-Young (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Cheon, Se-Yun (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Piao, Ren-Zhe (College of Agriculture, Yanbian University) ;
  • Lee, Sang-Woo (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Cha, Yun-Yeop (Department of Rehabilitative Medicine of Korean Medicine and Neuropsychiatry, College of Korean Medicine, Sangji University) ;
  • An, Hyo-Jin (Department of Pharmacology, College of Korean Medicine, Sangji University)
  • 박예진 (상지대학교 한의과대학 약리학교실) ;
  • 서종환 (상지대학교 한의과대학 약리학교실) ;
  • 길태영 (상지대학교 한의과대학 약리학교실) ;
  • 천세윤 (상지대학교 한의과대학 약리학교실) ;
  • 박인철 (옌벤대학교 농과대학) ;
  • 이상우 (한국생명공학연구원 해외생물소재센터) ;
  • 차윤엽 (상지대학교 한의과대학 재활의학교실) ;
  • 안효진 (상지대학교 한의과대학 약리학교실)
  • Received : 2018.10.10
  • Accepted : 2018.11.25
  • Published : 2018.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This study was fulfilled to investigate nominee materials as anti-inflammatory agent from ethanol extract of Chinese medicinal plants in Yanjin. Among the 20 candidates, we selected most effective one, the ethanol extract of Cicuta virosa L. (CVL). The mechanism underlying the anti-inflammatory effects of CVL is not clearly identified as yet. Accordingly, we clarified the anti-inflammatory effects of CVL and its underlying molecular mechanisms in LPS-stimulated RAW 264.7 macrophages. Methods : RAW264.7 macrophages were incubated with CVL (12.5, 25, or $50{\mu}M$) and/or lipopolysaccharide (LPS) ($1{\mu}g/m{\ell}$). Cytotoxicity was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and the level of nitric oxide (NO) production was measured with Griess reagent. The prostaglandin $E_2$ ($PGE_2$) production was measured with enzyme immunoassay kits and the protein expression of inducible nitric oxide synthase (iNOS) was determined using Western blot analysis. Results : Among the 20 ethanol extract of Chinese medicinal plants of Yanjin tested, CVL significantly reduced the production of NO in a dose-dependent manner via inhibition the protein expressions of iNOS without cytotoxicity on the LPS-stimulated RAW 264.7 macrophages. In addition, CVL also effectively declined the production of $PGE_2$ in LPS-simulated RAW 264.7 macrophages. Conclusions : Taken together, these data presented in this study demonstrate that CVL possesses anti-inflammatory activity by suppressing the production of pro-inflammatory mediators NO and $PGE_2$, and pro-inflammatory protein iNOS expression in LPS-stimulated RAW 264.7 macrophages.

Keywords

DHBCBU_2018_v33n6_71_f0001.tif 이미지

Figure 1. Effect of CVL on the viability of RAW 264.7 cells.

DHBCBU_2018_v33n6_71_f0002.tif 이미지

Figure 2. Effect of CVL on production of NO in LPS-stimulated RAW 264.7 cells.

DHBCBU_2018_v33n6_71_f0003.tif 이미지

Figure 3. Effect of CVL on production of PGE2 in LPS-stimulated RAW 264.7 cells.

DHBCBU_2018_v33n6_71_f0004.tif 이미지

Figure 4. Effect of CVL on iNOS protein expression in LPS-stimulated RAW 264.7 cells.

Table 1. Effect of Ethanol extract of Chinese medicinal plants in Yanjin on the cell viability in RAW 264.7 macrophages.

DHBCBU_2018_v33n6_71_t0001.tif 이미지

Table 2. Effect of Ethanol extract of Chinese medicinal plants in Yanjin on LPS-stimulated NO production level in RAW 264.7 macrophages.

DHBCBU_2018_v33n6_71_t0002.tif 이미지

References

  1. Choi, Y.H., G.Y. Kim, and H.H. Lee, Anti-inflammatory effects of cordycepin in lipopolysaccharide-stimulated RAW 264.7 macrophages through Toll-like receptor 4-mediated suppression of mitogen-activated protein kinases and NF-kappaB signaling pathways. Drug Des Devel Ther. 2014;8:1941-53.
  2. Lee, W., N. Kang, S.Y. Park, S.H. Cheong, K.J. Chang, S.H. Kim, J.H. Um, E.J. Han, E.A. Kim, Y.J. Jeon, and G. Ahn, Xylose-Taurine Reduced Suppresses the Inflammatory Responses in Lipopolysaccharide-Stimulated Raw264.7 Macrophages. Adv Exp Med Biol. 2017;975:633-642.
  3. Wang, P., Q. Qiao, J. Li, W. Wang, L.P. Yao, and Y.J. Fu, Inhibitory effects of geraniin on LPS-induced inflammation via regulating NF-kappaB and Nrf2 pathways in RAW 264.7 cells. Chem Biol Interact. 2016;253:134-42. https://doi.org/10.1016/j.cbi.2016.05.014
  4. Oh, Y.C., W.K. Cho, J.H. Oh, G.Y. Im, Y.H. Jeong, M.C. Yang, and J.Y. Ma, Fermentation by Lactobacillus enhances anti-inflammatory effect of Oyaksungisan on LPS-stimulated RAW 264.7 mouse macrophage cells. BMC Complement Altern Med. 2012;12:17.
  5. Lee, H.J., J.S. Shin, K.G. Lee, S.C. Park, Y.P. Jang, J.H. Nam, and K.T. Lee, Ethanol Extract of Potentilla supina Linne Suppresses LPS-induced Inflammatory Responses through NF-kappaB and AP-1 Inactivation in Macrophages and in Endotoxic mice. Phytother Res. 2017;31(3):475-487. https://doi.org/10.1002/ptr.5773
  6. Gasparrini, M., T.Y. Forbes-Hernandez, F. Giampieri, S. Afrin, J.M. Alvarez-Suarez, L. Mazzoni, B. Mezzetti, J.L. Quiles, and M. Battino, Anti-inflammatory effect of strawberry extract against LPS-induced stress in RAW 264.7 macrophages. Food Chem Toxicol. 2017;102:1-10. https://doi.org/10.1016/j.fct.2017.01.018
  7. Yang, H.L., S.W. Lin, C.C. Lee, K.Y. Lin, C.H. Liao, T.Y. Yang, H.M. Wang, H.C. Huang, C.R. Wu, and Y.C. Hseu, Induction of Nrf2-mediated genes by Antrodia salmonea inhibits ROS generation and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophages. Food Funct. 2015;6(1):230-41. https://doi.org/10.1039/c4fo00869c
  8. He, J., J. Li, H. Liu, Z. Yang, F. Zhou, T. Wei, Y. Dong, H. Xue, L. Tang, and M. Liu, Scandoside Exerts Anti-Inflammatory Effect Via Suppressing NF-kappaB and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Macrophages. Int J Mol Sci. 2018;19(2).
  9. Park, S.B., G.H. Park, H.N. Kim, H.J. Son, H.M. Song, H.S. Kim, H.J. Jeong, and J.B. Jeong, Anti-inflammatory effect of the extracts from the branch of Taxillus yadoriki being parasitic in Neolitsea sericea in LPS-stimulated RAW264.7 cells. Biomed Pharmacother. 2018;104:1-7. https://doi.org/10.1016/j.biopha.2018.05.034
  10. Damodar, K., J.T. Lee, J.K. Kim, and J.G. Jun, Synthesis and in vitro evaluation of homoisoflavonoids as potent inhibitors of nitric oxide production in RAW-264.7 cells. Bioorg Med Chem Lett. 2018;28(11):2098-2102. https://doi.org/10.1016/j.bmcl.2018.04.037
  11. Liang, N., Y. Sang, W. Liu, W. Yu, and X. Wang, Anti-Inflammatory Effects of Gingerol on Lipopolysaccharide-Stimulated RAW 264.7 Cells by Inhibiting NF-kappaB Signaling Pathway. Inflammation. 2018.
  12. Guo, C., L. Yang, J. Luo, C. Zhang, Y. Xia, T. Ma, and L. Kong, Sophoraflavanone G from Sophora alopecuroides inhibits lipopolysaccharide-induced inflammation in RAW264.7 cells by targeting PI3K/Akt, JAK/STAT and Nrf2/HO-1 pathways. Int Immunopharmacol. 2016;38:349-56. https://doi.org/10.1016/j.intimp.2016.06.021
  13. Choi, H.E., H.J. Kwak, S.K. Kim, and H.G. Cheon, Foenumoside B isolated from Lysimachia foenum-graecum extract suppresses LPS-induced inflammatory response via NF-kappaB/AP-1 inactivation in murine macrophages and in endotoxin-induced shock model. Eur J Pharmacol. 2018;832:120-128. https://doi.org/10.1016/j.ejphar.2018.05.022
  14. Huang, C., W. Li, Q. Zhang, L. Chen, W. Chen, H. Zhang, and Y. Ni, Anti-inflammatory activities of Guang-Pheretima extract in lipopolysaccharide-stimulated RAW 264.7 murine macrophages. BMC Complement Altern Med. 2018;18(1):46. https://doi.org/10.1186/s12906-018-2086-z
  15. Cordeiro Caillot, A.R., I. de Lacerda Bezerra, L. Palhares, A.P. Santana-Filho, S.F. Chavante, and G.L. Sassaki, Structural characterization of blackberry wine polysaccharides and immunomodulatory effects on LPS-activated RAW 264.7 macrophages. Food Chem. 2018;257:143-149. https://doi.org/10.1016/j.foodchem.2018.02.122
  16. Park, S.B., G.H. Park, Y. Um, H.N. Kim, H.M. Song, N. Kim, H.S. Kim, and J.B. Jeong, Wood-cultivated ginseng exerts anti-inflammatory effect in LPS-stimulated RAW264.7 cells. Int J Biol Macromol. 2018;116:327-334. https://doi.org/10.1016/j.ijbiomac.2018.05.039
  17. Ngo, Q.T., T.Q. Cao, P.L. Tran, J.A. Kim, S.T. Seo, J.C. Kim, M.H. Woo, J.H. Lee, and B.S. Min, Lactones from the pericarps of Litsea japonica and their anti-inflammatory activities. Bioorg Med Chem Lett. 2018;28(11):2109-2115. https://doi.org/10.1016/j.bmcl.2018.04.023
  18. Jin, S., J. Wang, S. Chen, A. Jiang, M. Jiang, Y. Su, W. Yan, Y. Xu, and G. Gong, A novel limonin derivate modulates inflammatory response by suppressing the TLR4/NF-kappaB signalling pathway. Biomed Pharmacother. 2018;100:501-508. https://doi.org/10.1016/j.biopha.2018.02.046
  19. Li, Y.R., C.S. Fu, W.J. Yang, X.L. Wang, D. Feng, X.N. Wang, D.M. Ren, H.X. Lou, and T. Shen, Investigation of constituents from Cinnamomum camphora (L.) J. Presl and evaluation of their anti-inflammatory properties in lipopolysaccharide-stimulated RAW 264.7 macrophages. J Ethnopharmacol. 2018;221:37-47. https://doi.org/10.1016/j.jep.2018.04.017
  20. Zhang, Y. and O.J. Igwe, Lipopolysaccharide (LPS)-mediated priming of toll-like receptor 4 enhances oxidant-induced prostaglandin E2 biosynthesis in primary murine macrophages. Int Immunopharmacol. 2018;54:226-237. https://doi.org/10.1016/j.intimp.2017.11.017
  21. Kim, E.A., S.Y. Kim, B.R. Ye, J. Kim, S.C. Ko, W.W. Lee, K.N. Kim, I.W. Choi, W.K. Jung, and S.J. Heo, Anti-inflammatory effect of Apo-9'-fucoxanthinone via inhibition of MAPKs and NF-kB signaling pathway in LPS-stimulated RAW 264.7 macrophages and zebrafish model. Int Immunopharmacol. 2018;59:339-346. https://doi.org/10.1016/j.intimp.2018.03.034