Figure 1. Schematic illustration of the preparation of amorphous calcium phosphate (ACP) nanoparticles via a hydrothermal method.
Figure 2. FESEM images of as-synthesized ACP nanoparticles. (A) ACP nanoparticles synthesized in the presence of MgCl2. Inset is a higher magnification FESEM image. (B) ACP nanoparticles synthesized in the presence of sodium phytate. (C) Crystalline hydroxyapatite (HAP) nanorods that were transformed from ACP nanoparticles synthesized with no additives after a short induction period. Inset is a higher magnification FESEM image.
Figure 3. FESEM images and their corresponding EDS spectra of as-synthesized ACP nanoparticles. (A) ACP nanoparticles synthesized with no additives. (B) ACP nanoparticles synthesized in the presence of MgCl2.
Figure 4. X-ray powder diffraction (XRD) patterns of ACP nanoparticles and HAP nanorods. Blue curve (ACP1) exhibits the XRD pattern of the ACP nanoparticles synthesized in the presence of MgCl2 (blue curve). Red curve (ACP2) shows the XRD pattern of the ACP particles synthesized in the presence of sodium phytate. Green curve (HAP) corresponding to the XRD pattern of HAP nanorods shows their characteristic peaks (denoted with asterisk marks) that are assigned based on JCPDS No. 09-0432 (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).
Figure 5. FT-IR spectra of as-synthesized ACP nanoparticles and HAP nanorods. Black curve corresponds to FT-IR of Na2ATP. Red (ACP1) and blue (ACP2) curves correspond to the spectra from the as-synthesized ACP nanoparticles in the presence of MgCl2 and sodium phytate, respectively. Green (HAP) curve corresponds to HAP nanorods that were transformed from ACP nanoparticles synthesized with no additive. The positions of characteristic ν3 and ν4 vibrations of PO43- groups were embodied with the arrows in each spectrum (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).
References
- C. Qi, J. Lin, L. H. Fu, and P. Huang, Calcium-based biomaterials for diagnosis, treatment, and theranostics, Chem. Soc. Rev., 47, 357-403 (2018). https://doi.org/10.1039/C6CS00746E
- C. Combes, S. Cazalbou, and C. Rey, Apatite biominerals, Minerals, 6, 1-25 (2016).
- W. J. Jin, S. Q. Jiang, H. H. Pan, and R. K. Tang, Amorphous phase mediated crystallization: Fundamentals of biomineralization, Crystals, 8, 1-24 (2018).
- H. R. Wang et al., Oriented and ordered biomimetic remineralization of the surface of demineralized dental enamel using HAP@ACP nanoparticles guided by glycine, Sci. Rep., 7, 40701-40713 (2017). https://doi.org/10.1038/srep40701
- E. Beniash, R. A. Metzler, R. S. K. Lam, and P. U. P. A. Gilbert, Transient amorphous calcium phosphate in forming enamel, J. Struct. Biol., 166, 133-143 (2009). https://doi.org/10.1016/j.jsb.2009.02.001
- A. Dey et al., The role of prenucleation clusters in surface-induced calcium phosphate crystallization, Nat. Mater., 9, 1010-1014 (2010). https://doi.org/10.1038/nmat2900
- H. Zhou and J. Lee, Nanoscale hydroxyapatite particles for bone tissue engineering, Acta Biomater., 7, 2769-2781 (2011). https://doi.org/10.1016/j.actbio.2011.03.019
- M. Nagano, T. Nakamura, T. Kokubo, M. Tanahashi, and M. Ogawa, Differences of bone bonding ability and degradation behavior in vivo between amorphous calcium phosphate and highly crystalline hydroxyapatite coating, Biomaterials, 17, 1771-1777 (1996). https://doi.org/10.1016/0142-9612(95)00357-6
- A. L. Boskey, Amorphous calcium phosphate: The contention of bone, J. Dent. Res., 76, 1433-1436 (1997). https://doi.org/10.1177/00220345970760080501
- S. Kim, H. S. Ryu, H. Shin, H. S. Jung, and K. S. Hong, In situ observation of hydroxyapatite nanocrystal formation from amorphous calcium phosphate in calcium-rich solutions, Mater. Chem. Phys., 91, 500-506 (2005). https://doi.org/10.1016/j.matchemphys.2004.12.016
- C. G. Wang et al., Crystallization at Multiple Sites inside Particles of Amorphous Calcium Phosphate, Cryst. Growth Des., 9, 2620-2626 (2009). https://doi.org/10.1021/cg801069t
- A. L. Boskey and A. S. Posner, Conversion of amorphous calcium phosphate to microcrystalline hydroxyapatite. A pH-dependent, solution- mediated, solid-solid conversion, J. Phys. Chem., 77, 2313-2317 (1973). https://doi.org/10.1021/j100638a011
- G. H. Nancollas and B. Tomazic, Growth of calcium-phosphate on hydroxyapatite crystals - Effect of supersaturation and ionic medium, J. Phys. Chem., 78, 2218-2225 (1974). https://doi.org/10.1021/j100615a007
- H. C. Margolis, S. Y. Kwak, and H. Yamazaki, Role of mineralization inhibitors in the regulation of hard tissue biomineralization: Relevance to initial enamel formation and maturation, Front. Physiol., 5:339 (2014).
- S. Q. Jiang, W. Jin, Y.-N. Wang, H. Pan, Z. Sun, and R. Tang, Effect of the aggregation state of amorphous calcium phosphate on hydroxyapatite nucleation kinetics, RSC Adv., 7, 25497-25503 (2017). https://doi.org/10.1039/C7RA02208E
- Z. Zyman, D. Rokhmistrov, and V. Glushko, Structural changes in precipitates and cell model for the conversion of amorphous calcium phosphate to hydroxyapatite during the initial stage of precipitation, J. Cryst. Growth, 353, 5-11 (2012). https://doi.org/10.1016/j.jcrysgro.2012.04.041
- H. Furedi-Milhofer, L. Brecevic, and B. Purgaric, Crystal growth and phase transformation in the precipitation of calcium phosphates, Faraday Discuss. Chem. Soc., 61, 184-193 (1976). https://doi.org/10.1039/DC9766100184
- S. Q. Jiang, H. H. Pan, Y. Chen, X. R. Xu, and R. K. Tang, Amorphous calcium phosphate phase-mediated crystal nucleation kinetics and pathway, Faraday Discuss., 179, 451-461 (2015). https://doi.org/10.1039/C4FD00212A
- R. Wuthier and E. Eanes, Effect of phospholipids on the transformation of amorphous calcium phosphate to hydroxyapatite in vitro, Calcif. Tissue Res., 19, 197-210 (1975). https://doi.org/10.1007/BF02564004
- R. Z. LeGeros et al., Amorphous calcium phosphates (ACP): Formation and stability, Key Eng. Mater., 284, 7-10 (2005).
- N. C. Blumenthal, F. Betts, and A. S. Posner, Stabilization of amorphous calcium-phosphate by Mg and ATP, Calcif. Tissue Res., 23, 245-250 (1977). https://doi.org/10.1007/BF02012793
- Y. Chen, W. J. Gu, H. H. Pan, S. Q. Jiang, and R. K. Tang, Stabilizing amorphous calcium phosphate phase by citrate adsorption, Cryst. Eng. Comm., 16, 1864-1867 (2014). https://doi.org/10.1039/C3CE42274G
- Z. Amjad, Inhibition of the amorphous calcium phosphate phase transformation reaction by polymeric and non-polymeric inhibitors, Phosphorus Res. Bull., 7, 45-54 (1997). https://doi.org/10.3363/prb1992.7.0_45
- C. Qi, Y.-J. Zhu, X.-Y. Zhao, B.-Q. Lu, Q.-L. Tang, J. Zhao, and F. Chen, Highly stable amorphous calcium phosphate porous nanospheres: Microwave-assisted rapid synthesis using ATP as phosphorus source and stabilizer, and their application in anticancer drug delivery, Chemistry, 19, 981-987 (2013). https://doi.org/10.1002/chem.201202829
- Y. Tanizawa and T. Suzuki, Effects of silicate ions on the formation and transformation of calcium phosphates in neutral aqueous solutions, J. Chem. Soc. Faraday Trans., 91, 3499-3503 (1995). https://doi.org/10.1039/ft9959103499
- P. Bar-Yosef Ofir, R. Govrin-Lippman, N. Garti, and H. Füredi-Milhofer, The influence of polyelectrolytes on the formation and phase transformation of amorphous calcium phosphate, Cryst. Growth Des., 4, 177-183 (2004). https://doi.org/10.1021/cg034148g
- M. J. Root, Inhibition of the amorphous calcium phosphate phase transformation reaction by polyphosphates and metal ions, Calcif. Tissue Int., 47, 112-116 (1990). https://doi.org/10.1007/BF02555994
- C. Qi, Q. L. Tang, Y. J. Zhu, X. Y. Zhao, and F. Chen, Microwave-assisted hydrothermal rapid synthesis of hydroxyapatite nanowires using adenosine 5'-triphosphate disodium salt as phosphorus source, Mater. Lett., 85, 71-73 (2012). https://doi.org/10.1016/j.matlet.2012.06.106
- F. Syberg, Y. Suveyzdis, C. Koetting, K. Gerwert, and E. Hofmann, Time-resolved Fourier transform infrared spectroscopy of the nucleotide-binding domain from the ATP-binding cassette transporter MsbA, J. Biol. Chem., 287, 23923-23931 (2012). https://doi.org/10.1074/jbc.M112.359208
-
M. Liu, M. Krasteva, and A. Barth, Interaction of phosphate groups of ATP and aspartyl phosphate with the Sarcoplasmic Reticulum
$Ca^{2+}$ -ATPase: A FTIR study, Biophys. J., 89, 4352-4363 (2005). https://doi.org/10.1529/biophysj.105.061689 - Z. F. Zhou et al., Calcium phosphate-phosphorylated adenosine hybrid microspheres for anti-osteosarcoma drug delivery and osteogenic differentiation, Biomaterials, 121, 1-14 (2017). https://doi.org/10.1016/j.biomaterials.2016.12.031
- Z. He, C. W. Honeycutt, T. Zhang, and P. M. Bertsch, Preparation and FT-IR characterization of metal phytate compounds, J. Environ. Qual., 35, 1319-1328 (2006). https://doi.org/10.2134/jeq2006.0008
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