Journal of Korean society of Dental Hygiene (한국치위생학회지)

Korean Society of Dental Hygiene (한국치위생학회)
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The antibacterial effect of Pleurotus eryngii extracts on oral bacteria

새송이버섯 추출물이 구강세균에 작용하는 항균효과

  • Chon, In-Young (Department of BT-Convergent Pharmaceutical Engineering, Sunmoon University) ;
  • Yu, Eun-Ji (Department of BT-Convergent Pharmaceutical Engineering, Sunmoon University) ;
  • Yu, Sang-Cheol (Department of Life Science and Biochemical Engineering, Graduate School, Sunmoon University) ;
  • Lee, Ji-Youn (Department of Dental Hygiene, GangneungYeongdong University) ;
  • Jung, Sang-Hee (Department of Dental Hygiene, GangneungYeongdong University) ;
  • Oh, Tae-Jin (Department of BT-Convergent Pharmaceutical Engineering, Sunmoon University)
  • 전인영 (선문대학교 건강보건대학 BT융합제약공학과) ;
  • 유은지 (선문대학교 건강보건대학 BT융합제약공학과) ;
  • 유상철 (선문대학교 일반대학원 생명공학과) ;
  • 이지연 (강릉영동대학교 치위생과) ;
  • 정상희 (강릉영동대학교 치위생과) ;
  • 오태진 (선문대학교 건강보건대학 BT융합제약공학과)
  • Received : 2017.10.11
  • Accepted : 2017.12.18
  • Published : 2018.02.28
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Abstract

Objectives: Pleurotus eryngii is used both for edible and medicinal purposes, and has a physiological activity. The purpose of this study is to investigate the antibacterial effect of Pleurotus eryngii against six oral pathogens (Staphylococcus aureus, Streptococcus criceti, Streptococcus mutans, Streptococcus ratti, Streptococcus sobrinus, and Actinomyces viscosus). Methods: The antibacterial activities of various extracts of Pleurotus eryngii were examined by disc diffusion assay and minimum inhibitory concentration (MIC). The disc diffusion assay was performed by putting a paper disc soaked in extracts on plates inoculated bacterial cultures. The MIC of these extracts was determined by using a broth microdilution assay at a concentration ranging between 0.03 mg/ml to 15.00 mg/ml. The growth inhibition effect of extracts was measured at 600 nm for 24 hrs. Results: The antibacterial activity was confirmed against all six tested bacteria at Pleurotus eryngii ethyl acetate extract by the disc diffusion method. Acetone extract showed the antibacterial activity only against 4 strains containing Streptococcus criceti, Streptococcus mutans, Streptococcus ratti, and Actinomyces viscosus. In ethanol extract, no activity was observed against other strains except Staphylococcus aureus. MIC values of ethyl acetate extract were the same, 7.50 mg/ml in all tested bacteria. Conclusions: Pleurotus eryngii exhibited the antibacterial activity against oral pathogens (Staphylococcus aureus, Streptococcus criceti, Streptococcus mutans, Streptococcus ratti, Streptococcus sobrinus, and Actinomyces viscosus). Thus, Pleurotus eryngii may be considered as a natural antibacterial agent for treatment of dental diseases.

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References

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