DOI QR코드

DOI QR Code

Assessments in biocides with omics approaches to ecosystem

  • Ma, Seohee (Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Yoon, Dahye (Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Kim, Hyunsu (Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Lee, Hyangjin (Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Kim, Seonghye (Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Lee, Huichan (Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Kim, Jieun (Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Lee, Soojin (Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Lee, Yunsuk (Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Lee, Yujin (Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Kim, Suhkmann (Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University)
  • 투고 : 2017.11.16
  • 심사 : 2018.12.01
  • 발행 : 2018.12.20

초록

Benzisothiazolinone (BIT) is the preservative that is widely used in industrial and household products. In this study, zebrafish (Danio rerio) was exposed to BIT at different concentrations (control, 0.5 g/L, 1.0 g/L and 2.0 g/L) for 72 hours. The techniques of nuclear magnetic resonance (NMR) spectroscopy were applied to analyze the effects of BIT on zebrafish. The advantages of NMR are the minimal sample preparation and high reproducibility of experimental results. With the multivariate statistical analysis, dimethylamine, N-acetylaspartate, glycine and histidine were identified as an important metabolite in differentiating between the control and BIT-exposed group. This study will improve the understanding the metabolite changes in the zebrafish in response to BIT exposure.

키워드

JGGMB2_2018_v22n4_91_f0001.png 이미지

Figure 1. Representative 1H-NMR spectrum of aqueous extracts of zebrafish. High and low field spectra were magnified. Ala, Gln and Glu indicate alanine, glutamine and glutamate, respectively.

JGGMB2_2018_v22n4_91_f0002.png 이미지

Figure 2. Data normalization plot (control-based z-score).

JGGMB2_2018_v22n4_91_f0003.png 이미지

Figure 2. PLS-DA score plot of zebrafish exposed to BIT (●, control; ●, exposure).

JGGMB2_2018_v22n4_91_f0004.png 이미지

Figure 4. VIP plot. The VIP plot displays the top 15 most important metabolite features identified by PLS-DA. (Metabolites of VIP > 1).

JGGMB2_2018_v22n4_91_f0005.png 이미지

Figure 5. ROC curve. The ROC curves are the comparison of control and BIT exposed group. AUC values: Dimethylamine (0.96) and N-Acetylaspartate (0.92).

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