Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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High-performance liquid chromatography analysis of phytosterols in Panax ginseng root grown under different conditions

  • Lee, Dong Gu (Department of Integrative Plant Science, Chung-Ang University) ;
  • Lee, Jaemin (Department of Integrative Plant Science, Chung-Ang University) ;
  • Kim, Kyung-Tack (Korea Food Research Institute) ;
  • Lee, Sang-Won (Department of Medicinal Crop Research Institute, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Young-Ock (Department of Medicinal Crop Research Institute, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Cho, Ik-Hyun (Department of Convergence Medical Science, Brain Korea 21 Plus Program, and Institute of Korean Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Hak-Jae (Department of Clinical Pharmacology, College of Medicine, Soonchunhyang University) ;
  • Park, Chun-Gun (Department of Medicinal Crop Research Institute, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University)
  • Received : 2016.04.18
  • Accepted : 2016.10.25
  • Published : 2018.01.15
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Abstract

Background: The Panax ginseng plant is used as an herbal medicine. Phytosterols of P. ginseng have inhibitory effects on inflammation-related factors in HepG2 cells. Methods: Phytosterols (e.g., stigmasterol and ${\beta}$-sitosterol) in the roots of P. ginseng grown under various conditions were analyzed using high-performance liquid chromatography. The P. ginseng roots analyzed in this study were collected from three cultivation areas in Korea (i.e., Geumsan, Yeongju, and Jinan) and differed by cultivation year (i.e., 4 years, 5 years, and 6 years) and production process (i.e., straight ginseng, red ginseng, and white ginseng). Results: The concentrations of stigmasterol and ${\beta}$-sitosterol in P. ginseng roots were 2.22-23.04 mg/g and 7.35-59.09 mg/g, respectively. The highest concentrations of stigmasterol and ${\beta}$-sitosterol were in the roots of 6-year-old P. ginseng cultivated in Jinan (82.14 mg/g and 53.23 mg/g, respectively). Conclusion: Six-year-old white ginseng and white ginseng cultivated in Jinan containing stigmasterol and b-sitosterol are potentially a new source of income in agriculture.

Keywords

References

  1. Attele AS, Wu JA, Yuan CS. Ginseng pharmacology: multiple constituents and multiple actions. Biochem Pharmacol 1999;58:1685-93. https://doi.org/10.1016/S0006-2952(99)00212-9
  2. Hofseth LJ, Wargovich MJ. Inflammation, cancer, and targets of ginseng. J Nutr 2007;137(1 Suppl.):183S-5S. https://doi.org/10.1093/jn/137.1.183S
  3. Kim SK, Park JH. Trends in ginseng research in 2010. J Ginseng Res 2011;35: 389-98. https://doi.org/10.5142/jgr.2011.35.4.389
  4. Sun BS, Gu LJ, Fang ZM, Wang CY, Wang Z, Lee MR, Li Z, Li JJ, Sung CK. Simultaneous quantification of 19 ginsenosides in black ginseng developed from Panax ginseng by HPLC-ELSD. J Pharm Biomed Anal 2009;50:15-22. https://doi.org/10.1016/j.jpba.2009.03.025
  5. Chen CF, Chiou WF, Zhang JT. Comparison of the pharmacological effects of Panax ginseng and Panax quinquefolium. Acta Pharmacol Sin 2008;29:1103-8. https://doi.org/10.1111/j.1745-7254.2008.00868.x
  6. Yue PY, Mak NK, Cheng YK, Leung KW, Ng TB, Fan DT, Yeung HW, Wong RN. Pharmacogenomics and the yin/yang actions of ginseng: anti-tumor, angiomodulating and steroid-like activities of ginsenosides. Chin Med 2007;2:1-21. https://doi.org/10.1186/1749-8546-2-1
  7. Jung CH, Seog HM, Choi IW, Cho HY. Antioxidant activities of cultivated and wild Korean ginseng leaves. Food Chem 2005;92:535-40. https://doi.org/10.1016/j.foodchem.2004.08.021
  8. Rai D, Bhatia G, Sen T, Palit G. Anti-stress effects of Ginkgo biloba and Panax ginseng: a comparative study. J Pharmacol Sci 2003;4:458-64.
  9. Choi S. Epidermis proliferative effect of the Panax ginseng ginsenoside Rb2. Arch Pharm Res 2002;25:71-6. https://doi.org/10.1007/BF02975265
  10. Surh YJ, Na HK, Lee JY, Keum YS. Molecular mechanisms underlying antitumor promoting activities of heat-processed Panax ginseng C. A. Meyer. J Kor Med Sci 2001;16:38-41. https://doi.org/10.3346/jkms.2001.16.S.S38
  11. Chang LK, Whitaker DC. The impact of herbal medicine on dermatologic surgery. Dermatol Surg 2001;27:759-63.
  12. Keum YS, Park KK, Lee JM, Chun KS, Park JH, Lee SK, Kwon H, Surh YJ. Antioxidant and anti-tumor promoting activities of the methanol extract of heatprocessed ginseng. Cancer Lett 2000;150:41-8. https://doi.org/10.1016/S0304-3835(99)00369-9
  13. Jones PJH, MacDougall DE, Ntanios F, Vanstone CA. Dietary phytosterols as cholesterol-lowering agents in humans. Can J Physiol Pharmacol 1997;75: 217-27. https://doi.org/10.1139/y97-011
  14. Miettinen TA, Puska P, Gylling H, Vanhanen H, Vartiainen E. Reduction of serum cholesterol with sitostanol-ester margarine in a mildly hypercholesterolemic population. N Engl J Med 1995;333:1308-12. https://doi.org/10.1056/NEJM199511163332002
  15. Ronco A, De Stefani E, Boffetta P, Deneo-Pellegrini H, Mendilaharsu M, Leborgne F. Vegetables, fruits, and related nutrients and risk of breast cancer: a case-control study in Uruguay. Nutr Cancer 1999;35:111-9. https://doi.org/10.1207/S15327914NC352_3
  16. Berges RR, Windeler J, Trampisch HJ, Senge T. Randomised, placebocontrolled, double-blind clinical trial of ${\beta}$-sitosterol in patients with benign prostatic hyperplasia. ${\beta}$-Sitosterol study group. Lancet 1995;345:1529-32. https://doi.org/10.1016/S0140-6736(95)91085-9
  17. Akihisa T, Yasukawa K, Yamaura M, Ukiya M, Kimura Y, Shimizu N, Arai K. Triterpene alcohol and sterol ferulates from rice bran and their antiinflammatory effects. J Agric Food Chem 2000;48:2313-9. https://doi.org/10.1021/jf000135o
  18. Arisawa M, Kinghorn AD, Cordell GA, Phoebe CH, Farnsworth NR. Plant anticancer agents. XXXVI. Schottenol glucoside from Baccharis cordifolia and Ipomopsis aggregata. Planta Med 1985;6:544-5.
  19. Recio MC, Giner RM, Manez S, Rios JL. Structural requirements for the antiinflammatory activity of natural triterpenoids. Planta Med 1995;61:182-5. https://doi.org/10.1055/s-2006-958045
  20. Ling WH, Jones PJ. Dietary Phytosterols: a review of metabolism, benefits and side effects. Life Sci 1995;57:195-206. https://doi.org/10.1016/0024-3205(95)00263-6
  21. Kobayashi Y, Sugaya Y, Tokue A. Clinical effects of ${\beta}$-sitosterol (phytosterol) on benign prostatic hyperplasia: preliminary study. Hinyokika Kiyo 1998;44: 865-8 [in Japanese].
  22. Pegel KH. The importance of sitosterol and sitosterol in human and animal nutrition. S Afr J Sci 1997;93:263-8.
  23. Lee DG, Kim KT, Lee S. Taste profile characterization of white ginseng by electronic tongue analysis. Afr J Biotechnol 2012;11:9280-7.
  24. Lee DG, Lee AY, Kim KT, Cho EJ, Lee S. Novel dammarane-type triterpene saponins from Panax ginseng root. Chem Pharm Bull (Tokyo) 2015;63:927-34. https://doi.org/10.1248/cpb.c15-00302
  25. Chang IM, Yun HS, Yamasaki K. Revision of $^{13}C$- NMR assignments of bsitosterol and ${\beta}$-sitostery-3-O-${\beta}$-d-glucopyranoside isolated from Plantago asiatica seed. Kor J Pharmacogn 1981;12:12-4.
  26. Chaturvedula VSP, Prakash I. Isolation of stigmasterol and ${\beta}$-sitosterol from the dichloromethane extract of Rubus suavissimus. Int Curr Pharm J 2012;1: 239-42.
  27. Panda S, Jafri M, Kar A, Meheta BK. Thyroid inhibitory, antiperoxidative and hypoglycemic effects of stigmasterol isolated from Butea monosperma. Fitoterapia 2009;80:123-6. https://doi.org/10.1016/j.fitote.2008.12.002
  28. Jamaluddin F, Mohamed S, Lajis MN. Hypoglycaemic effect of Parkia speciosa seeds due to the synergistic action of ${\beta}$-sitosterol and stigmasterol. Food Chem 1994;49:339-45. https://doi.org/10.1016/0308-8146(94)90002-7
  29. Bouic PJ. Sterols and sterolins: new drugs for the immune system? Drug Discov Today 2002;7:775-8. https://doi.org/10.1016/S1359-6446(02)02343-7
  30. Cho IH. Volatile compounds of ginseng (Panax sp.): a review. J Korean Soc Appl Biol Chem 2015;58:67-75. https://doi.org/10.1007/s13765-015-0007-0
  31. Beveridge TH, Li TS, Drover JC. Phytosterol content in American ginseng seed oil. J Agric Food Chem 2002;50:744-50. https://doi.org/10.1021/jf010701v
  32. Kim JA, Son JH, Song SB, Yang SY, Kim YH. Sterols isolated from seeds of Panax ginseng and their anti-inflammatory activities. Pharmacogn Mag 2013;9:182-5. https://doi.org/10.4103/0973-1296.111288

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