Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Protective effect of ginsenoside Rb1 against tacrolimus-induced apoptosis in renal proximal tubular LLC-PK1 cells

  • Lee, Dahae (School of Pharmacy, Sungkyunkwan University) ;
  • Lee, Dong-Soo (Institute of Human-Environment Interface Biology, Biomedical Research Institute, Department of Dermatology, Seoul National University College of Medicine) ;
  • Jung, Kiwon (College of Pharmacy, CHA University) ;
  • Hwang, Gwi Seo (College of Korean Medicine, Gachon University) ;
  • Lee, Hye Lim (College of Korean Medicine, Gachon University) ;
  • Yamabe, Noriko (College of Korean Medicine, Gachon University) ;
  • Lee, Hae-Jeong (Department of Food and Nutrition, Gachon University) ;
  • Eom, Dae-Woon (Department of Pathology, University of Ulsan College of Medicine) ;
  • Kim, Ki Hyun (School of Pharmacy, Sungkyunkwan University) ;
  • Kang, Ki Sung (College of Korean Medicine, Gachon University)
  • Received : 2016.09.06
  • Accepted : 2016.12.26
  • Published : 2018.01.15
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Abstract

Background: The aim of the present study was to evaluate the potential protective effects of six ginsenosides (Rb1, Rb2, Rc, Rd, Rg1, and Rg3) isolated from Panax ginseng against tacrolimus (FK506)-induced apoptosis in renal proximal tubular LLC-PK1 cells. Methods: LLC-PK1 cells were treated with FK506 and ginsenosides, and cell viability was measured. Protein expressions of mitogen-activated protein kinases, caspase-3, and kidney injury molecule-1 (KIM-1) were evaluated by Western blotting analyses. The number of apoptotic cells was measured using an image-based cytometric assay. Results: Reduction in cell viability by $60{\mu}M$ FK506 was ameliorated significantly by cotreatment with ginsenosides Rg1 and Rb1. The phosphorylation of p38, extracellular signal-regulated kinases, and KIM-1, and cleavage of caspase-3, increased markedly in LLC-PK1 cells treated with FK506 and significantly decreased after cotreatment with ginsenoside Rb1. The number of apoptotic cells decreased by 6.0% after cotreatment with ginsenoside Rb1 ($10{\mu}M$ and $50{\mu}M$). Conclusion: The antiapoptotic effects of ginsenoside Rb1 on FK506-induced apoptosis were mediated by the inhibition of mitogen-activated protein kinases and caspase activation.

Keywords

References

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