Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Genome-wide association study of carcass weight in commercial Hanwoo cattle

  • Edea, Zewdu (Department of Animal Science, Chungbuk National University) ;
  • Jeoung, Yeong Ho (Korea Animal Improvement Association) ;
  • Shin, Sung-Sub (Korea Institute for Animal products Quality Evaluation) ;
  • Ku, Jaeul (Biomedical Research Center, Turbosoft Inc.) ;
  • Seo, Sungbo (Biomedical Research Center, Turbosoft Inc.) ;
  • Kim, Il-Hoi (Department of Animal Science, Chungbuk National University) ;
  • Kim, Sang-Wook (Department of Animal Science, Chungbuk National University) ;
  • Kim, Kwan-Suk (Department of Animal Science, Chungbuk National University)
  • Received : 2017.04.11
  • Accepted : 2017.10.22
  • Published : 2018.03.01
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Abstract

Objective: The objective of the present study was to validate genes and genomic regions associated with carcass weight using a low-density single nucleotide polymorphism (SNP) Chip in Hanwoo cattle breed. Methods: Commercial Hanwoo steers (n = 220) were genotyped with 20K GeneSeek genomic profiler BeadChip. After applying the quality control of criteria of a call rate ${\geq}90%$ and minor allele frequency (MAF) ${\geq}0.01$, a total of 15,235 autosomal SNPs were left for genome-wide association (GWA) analysis. The GWA tests were performed using single-locus mixed linear model. Age at slaughter was fitted as fixed effect and sire included as a covariate. The level of genome-wide significance was set at $3.28{\times}10^{-6}$ (0.05/15,235), corresponding to Bonferroni correction for 15,235 multiple independent tests. Results: By employing EMMAX approach which is based on a mixed linear model and accounts for population stratification and relatedness, we identified 17 and 16 loci significantly (p<0.001) associated with carcass weight for the additive and dominant models, respectively. The second most significant (p = 0.000049) SNP (ARS-BFGL-NGS-28234) on bovine chromosome 4 (BTA4) at 21 Mb had an allele substitution effect of 43.45 kg. Some of the identified regions on BTA2, 6, 14, 22, and 24 were previously reported to be associated with quantitative trait loci for carcass weight in several beef cattle breeds. Conclusion: This is the first genome-wide association study using SNP chips on commercial Hanwoo steers, and some of the loci newly identified in this study may help to better DNA markers that determine increased beef production in commercial Hanwoo cattle. Further studies using a larger sample size will allow confirmation of the candidates identified in this study.

Keywords

References

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