Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of dietary supplementation of lipid-coated zinc oxide on intestinal mucosal morphology and expression of the genes associated with growth and immune function in weanling pigs

  • Song, Young Min (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Kim, Myeong Hyeon (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Kim, Ha Na (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Jang, Insurk (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Han, Jeong Hee (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Fontamillas, Giselle Ann (Graduate School of International Agricultural Technology, and Institute of Green Bio Science and Technology, Seoul National University) ;
  • Lee, Chul Young (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Park, Byung-Chul (Graduate School of International Agricultural Technology, and Institute of Green Bio Science and Technology, Seoul National University)
  • Received : 2017.09.28
  • Accepted : 2017.12.17
  • Published : 2018.03.01
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Abstract

Objective: The present study was conducted to investigate the effects of a lipid-coated zinc oxide (ZnO) supplement Shield Zn (SZ) at the sub-pharmacological concentration on intestinal morphology and gene expression in weanling pigs, with an aim to gain insights into the mechanism of actions for SZ. Methods: Forty 22-day-old weanling pigs were fed a nursery diet supplemented with 100 or 2,500 mg Zn/kg with uncoated ZnO (negative control [NC] or positive control [PC], respectively), 100, 200, or 400 mg Zn/kg with SZ for 14 days and their intestinal tissues were taken for histological and molecular biological examinations. The villus height (VH) and crypt depth (CD) of the intestinal mucosa were measured microscopically following preparation of the tissue specimen; expression of the genes associated with growth and immune function was determined using the real-time quantitative polymerase chain reaction. Results: There was no difference in daily gain, gain:feed, and diarrhea score between the SZ group and either of NC and PC. The VH and VH:CD ratio were less for the SZ group vs NC in the jejunum and duodenum, respectively (p<0.05). The jejunal mucosal mRNA levels of insulin-like growth factor (IGF-I) and interleukin (IL)-10 regressed and tended to regress (p = 0.053) on the SZ concentration with a positive coefficient, respectively, whereas the IL-6 mRNA level regressed on the SZ concentration with a negative coefficient. The mRNA levels of IGF-I, zonula occludens protein-1, tumor necrosis $factor-{\alpha}$, IL-6, and IL-10 did not differ between the SZ group and either of NC and PC; the occludin and transforming growth $factor-{\beta}1$ mRNA levels were lower for the SZ group than for PC. Conclusion: The present results are interpreted to suggest that dietary ZnO provided by SZ may play a role in intestinal mucosal growth and immune function by modulating the expression of IGF-I, IL-6, and IL-10 genes.

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References

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