Asian-Australasian Journal of Animal Sciences

  • 제31권4호
  • /
  • Pages.467-472
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  • 2018
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Molecular cloning and expression analysis of annexin A2 gene in sika deer antler tip

  • Xia, Yanling (College of Wildlife Resources, Northeast Forestry University) ;
  • Qu, Haomiao (College of Wildlife Resources, Northeast Forestry University) ;
  • Lu, Binshan (College of Wildlife Resources, Northeast Forestry University) ;
  • Zhang, Qiang (College of Wildlife Resources, Northeast Forestry University) ;
  • Li, Heping (College of Wildlife Resources, Northeast Forestry University)
  • 투고 : 2017.04.11
  • 심사 : 2017.08.03
  • 발행 : 2018.04.01
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초록

Objective: Molecular cloning and bioinformatics analysis of annexin A2 (ANXA2) gene in sika deer antler tip were conducted. The role of ANXA2 gene in the growth and development of the antler were analyzed initially. Methods: The reverse transcriptase polymerase chain reaction (RT-PCR) was used to clone the cDNA sequence of the ANXA2 gene from antler tip of sika deer (Cervus Nippon hortulorum) and the bioinformatics methods were applied to analyze the amino acid sequence of Anxa2 protein. The mRNA expression levels of the ANXA2 gene in different growth stages were examined by real time reverse transcriptase polymerase chain reaction (real time RT-PCR). Results: The nucleotide sequence analysis revealed an open reading frame of 1,020 bp encoding 339 amino acids long protein of calculated molecular weight 38.6 kDa and isoelectric point 6.09. Homologous sequence alignment and phylogenetic analysis indicated that the Anxa2 mature protein of sika deer had the closest genetic distance with Cervus elaphus and Bos mutus. Real time RT-PCR results showed that the gene had differential expression levels in different growth stages, and the expression level of the ANXA2 gene was the highest at metaphase (rapid growing period). Conclusion: ANXA2 gene may promote the cell proliferation, and the finding suggested Anxa2 as an important candidate for regulating the growth and development of deer antler.

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참고문헌

  1. Price JS, Allen S, Faucheux C, Althnaian T, Mount JG. Deer antlers: a zoological curiosity or the key to understanding organ regeneration in mammals? J Anat 2005;207:603-18. https://doi.org/10.1111/j.1469-7580.2005.00478.x
  2. Li CY, Yang FH, Li GY, et al. Antler regeneration: a dependent process of stem tissue primed via interaction with its enveloping skin. J Exp Zool 2007;307:95-105.
  3. Brockes JP, Kumar A. Appendage regeneration in adult vertebrates and implications for regenerative medicine. Science 2005;310:1919-23.
  4. Lord EA, Martin SK, Gray JP, Li CY, Clark DE. Cell cycle genes PEDF and CDKN1C in growing deer antlers. Anat Rec (Hoboken) 2007;290:994-1004. https://doi.org/10.1002/ar.20562
  5. Zhang, F, Wang ZY, Yuan J, et al. RNAi-mediated silencing of Anxa2 inhibits breast cancer cell proliferation by downregulating cyclin D1 in STAT3-dependent pathway. Breast Cancer Res Treat 2015;153:263-75. https://doi.org/10.1007/s10549-015-3529-6
  6. Maule F, Bresolin S, Rampazzo E, et al. Annexin 2A sustains glioblastoma cell dissemination and proliferation. Oncotarget 2016;7:54632-49.
  7. Lokman NA, Ween MP, Oehler MK, Ricciardelli C. The role of annexin A2 in tumorigenesis and cancer progression. Cancer Microenviron 2011;4:199-208.
  8. Zhang X, Liu S, Guo C, Zong J, Sun MZ. The association of annexin A2 and cancers. Clin Transl Oncol 2012;14:634-40. https://doi.org/10.1007/s12094-012-0855-6
  9. Li CY, Clark DE, Lord EA, Stanton JA, Suttie JM. Sampling technique to discriminate the different tissue layers of growing antler tips for gene discovery. Anat Rec 2002;268:125-30. https://doi.org/10.1002/ar.10120
  10. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the $2^{−{\Delta}{\Delta}CT}$ method. Methods 2001;25:402-8. https://doi.org/10.1006/meth.2001.1262
  11. Jost M, Gerke V. Mapping of a regulatory important site for protein kinase C phosphorylation in the N-terminal domain of annexin ii. Biochim Biophys Acta 1996;1313:283-9. https://doi.org/10.1016/0167-4889(96)00101-2
  12. Liu J, Rothermund CA, Ayala-Sanmartin J, Vishwanatha JK. Nuclear annexin ii negatively regulates growth of LNCaP cells and substitution of ser 11 and 25 to glu prevents nucleo-cytoplasmic shuttling of annexin ii. BMC Biochem 2003;4:10.
  13. Grewal T, Enrich C. Annexins-modulators of EGF receptor signalling and trafficking. Cell Signal 2009;21:847-58.
  14. Ackland ML, Newgreen DF, Fridman M, et al. Epidermal growth factor-induced epithelio-mesenchymal transition in human breast carcinoma cells. Lab invest 2003;83:435-48. https://doi.org/10.1097/01.LAB.0000059927.97515.FD
  15. Shetty PK, Thamake Si, Biswas S, Johansson SL, Vishwanatha JK. Reciprocal regulation of annexin A2 and EGFR with Her-2 in Her-2 negative and herceptin-resistant breast cancer. PLoS ONE 2012;7(9):e44299. https://doi.org/10.1371/journal.pone.0044299
  16. Molnar A, Gyurjan i, Korpos E, et al. identification of differentially expressed genes in the development antler of red deer (Cervus elaphus). Mol Genet Genomics 2007;277:237-48. https://doi.org/10.1007/s00438-006-0193-x