Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Characterization of a Thermophilic Lignocellulose-Degrading Microbial Consortium with High Extracellular Xylanase Activity

  • Zhang, Dongdong (Institute of Marine Biology, Ocean College, Zhejiang University) ;
  • Wang, Yi (Institute of Agricultural Products Processing and Nuclear Agriculture Technology Research, Hubei Academy of Agricultural Sciences) ;
  • Zhang, Chunfang (Institute of Marine Biology, Ocean College, Zhejiang University) ;
  • Zheng, Dan (Institute of Agricultural Products Processing and Nuclear Agriculture Technology Research, Hubei Academy of Agricultural Sciences) ;
  • Guo, Peng (Institute of Agricultural Products Processing and Nuclear Agriculture Technology Research, Hubei Academy of Agricultural Sciences) ;
  • Cui, Zongjun (College of Agronomy and Biotechnology, China Agricultural University)
  • Received : 2017.09.18
  • Accepted : 2017.10.30
  • Published : 2018.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

A microbial consortium, TMC7, was enriched for the degradation of natural lignocellulosic materials under high temperature. TMC7 degraded 79.7% of rice straw during 15 days of incubation at $65^{\circ}C$. Extracellular xylanase was effectively secreted and hemicellulose was mainly degraded in the early stage (first 3 days), whereas primary decomposition of cellulose was observed as of day 3. The optimal temperature and initial pH for extracellular xylanase activity and lignocellulose degradation were $65^{\circ}C$ and between 7.0 and 9.0, respectively. Extracellular xylanase activity was maintained above 80% and 85% over a wide range of temperature ($50-75^{\circ}C$) and pH values (6.0-11.0), respectively. Clostridium likely had the largest contribution to lignocellulose conversion in TMC7 initially, and Geobacillus, Aeribacillus, and Thermoanaerobacterium might have also been involved in the later phase. These results demonstrate the potential practical application of TMC7 for lignocellulosic biomass utilization in the biotechnological industry under hot and alkaline conditions.

Keywords

References

  1. Kaparaju P, Serrano M, Thomsen AB, Kongjan P, Angelidaki I. 2009. Bioethanol, biohydrogen and biogas production from wheat straw in a biorefinery concept. Bioresour. Technol. 100: 2562-2568.
  2. Jimenez-Quero A, Pollet E, Zhao M, Marchioni E, Averous L, Phalip V. 2017. Fungal fermentation of lignocellulosic biomass for itaconic and fumaric acid production. J. Microbiol. Biotechnol. 27: 1-8. https://doi.org/10.4014/jmb.1607.07057
  3. Xin F, He J. 2013. Characterization of a thermostable xylanase from a newly isolated Kluyvera species and its application for biobutanol production. Bioresour. Technol. 135: 309-315. https://doi.org/10.1016/j.biortech.2012.10.002
  4. Himmel ME, Ding S, Johnson DK, Adney WS, Nimlos MR, Brady JW, et al. 2007. Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315: 804-807. https://doi.org/10.1126/science.1137016
  5. Yan X, Geng A, Zhang J, Wei Y, Zhang L, Qian C, et al. 2013. Discovery of (hemi-) cellulose genes in a metagenomics library from a biogas digester using 454 pyrosequencing. Appl. Microbiol. Biotechnol. 97: 8173-8182. https://doi.org/10.1007/s00253-013-4927-5
  6. Khandeparker R, Numan MT. 2008. Bifunctional xylanases and their potential use in biotechnology. J. Ind. Microbiol. Biotechnol. 35: 635-644. https://doi.org/10.1007/s10295-008-0342-9
  7. Hu J, Arantes V, Saddler JN. 2011. The enhancement of enzymatic hydrolysis of lignocellulosic substrates by the addition of accessory enzymes such as xylanase: is it an additive or synergistic effect? Biotechnol. Biofuels 4: 36. https://doi.org/10.1186/1754-6834-4-36
  8. Saha BC. 2003. Hemicellulose bioconversion. J. Ind. Microbiol. Biotechnol. 30: 279-291.
  9. Kumar R, Singh S, Singh OV. 2008. Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives. J. Ind. Microbiol. Biotechnol. 35: 377-391. https://doi.org/10.1007/s10295-008-0327-8
  10. Duff SJB, Murray WD. 1996. Bioconversion of forest products industry waste cellulosics to fuel ethanol: a review. Bioresour. Technol. 55: 1-33. https://doi.org/10.1016/0960-8524(95)00122-0
  11. Beg Q, Kapoor M, Mahajan L, Hoondal G. 2001. Microbial xylanases and their industrial applications: a review. Appl. Microbiol. Biotechnol. 56: 326-338. https://doi.org/10.1007/s002530100704
  12. Maalej I, Belhaj I, Masmoudi NF, Belghith H. 2009. Highly thermostable xylanase of the thermophilic fungus Talaromyces thermophilus: purification and characterization. Appl. Biochem. Biotechnol. 158: 200-212.
  13. Madlala AM, Bissoon S, Singh S, Christov L. 2001. Xylanaseinduced reduction of chlorine dioxide consumption during elemental chlorine-free bleaching of different pulp types. Biotechnol. Lett. 23: 345-351. https://doi.org/10.1023/A:1005693205016
  14. Guo P, Zhu W, Wang H, Lv Y, Wang X, Zheng D, Cui Z. 2010. Functional characteristics and diversity of a novel lignocelluloses degrading composite microbial system with high xylanase activity. J. Microbiol. Biotechnol. 20: 254-264.
  15. Zhang D, Wang Y, Zheng D, Guo P, Cheng W, Cui Z. 2016. New combination of xylanolytic bacteria isolated from the lignocellulose degradation microbial consortium XDC-2 with enhanced xylanase activity. Bioresour. Technol. 221: 686-690. https://doi.org/10.1016/j.biortech.2016.09.087
  16. Kato S, Haruta S, Cui Z, Ishii M, Yokota A, Igarashi Y. 2004. Clostridium straminisolvens sp. nov., a moderately thermophilic, aerotolerant and cellulolytic bacterium isolated from a cellulose-degrading bacterial community. Int. J. Syst. Evol. Microbiol. 54: 2043-2047. https://doi.org/10.1099/ijs.0.63148-0
  17. Wongwilaiwalin S, Rattanachomsri U, Laothanachareon T, Eurwilaichitr L, Igarashi Y, Champreda V. 2010. Analysis of a thermophilic lignocellulose degrading microbial consortium and multi-species lignocellulolytic enzyme system. Enzyme Microb. Technol. 47: 283-290. https://doi.org/10.1016/j.enzmictec.2010.07.013
  18. Feng Y, Yu Y, Wang X, Qu Y, Li D, He W, et al. 2011. Degradation of raw corn stover powder (RCSP) by an enriched microbial consortium and its community structure. Bioresour. Technol. 102: 742-747. https://doi.org/10.1016/j.biortech.2010.08.074
  19. Subramaniyan S, Prema P. 2002. Biotechnology of microbial xylanases: enzymology, molecular biology, and application. Crit. Rev. Biotechnol. 22: 33-64. https://doi.org/10.1080/07388550290789450
  20. Haruta S, Cui Z, Huang Z, Li M, Ishii M, Igarashi Y. 2002. Construction of a stable microbial community with high cellulose-degradation ability. Appl. Microbiol. Biotechnol. 59: 529-534. https://doi.org/10.1007/s00253-002-1026-4
  21. Bailey MJ, Biely P, Poutanen K. 1992. Interlaboratory testing of methods for assay of xylanase activity. J. Biotechnol. 23: 257-270. https://doi.org/10.1016/0168-1656(92)90074-J
  22. Ghose TK. 1987. Measurement of cellulase activities. Pure Appl. Chem. 59: 257-268. https://doi.org/10.1351/pac198759020257
  23. Zeng X, Borole AP, Pavlostathis SG. 2015. Biotransformation of furanic and phenolic compounds with hydrogen gas production in a microbial electrolysis cell. Environ. Sci. Technol. 49: 13667-13675.
  24. Liu J, Wang W, Yang H, Wang X, Gao L, Cui Z. 2006. Process of rice straw degradation and dynamic trend of pH by the microbial community MC1. J. Environ. Sci. 18: 1142-1146.
  25. Lv Z, Yang J, Yuan H. 2008. Production, purification and characterization of an alkaliphilic endo-${\beta}$-1,4-xylanase from a microbial community EMSD5. Enzyme Microb. Technol. 43: 343-348.
  26. Yang H, Wu H, Wang X, Cui Z, Li Y. 2011. Selection and characteristics of a switchgrass-colonizing microbial community to produce extracellular cellulases and xylanases. Bioresour. Technol. 102: 3546-3550. https://doi.org/10.1016/j.biortech.2010.09.009
  27. Wang H, Li J, Lv Y, Guo P, Wang X, Mochidzuki K, Cui Z. 2013. Bioconversion of un-pretreated lignocellulosic materials by a microbial consortium XDC-2. Bioresour. Technol. 136: 481-487. https://doi.org/10.1016/j.biortech.2013.03.015
  28. Bayer EA, Kenig R, Lamed R. 1983. Adherence of Clostridium thermocellum to cellulose. J. Bacteriol. 156: 818-827.
  29. Schellenberg JJ, Verbeke TJ, McQueen P, Krokhin OV, Zhang X, Alvare G, et al. 2014. Enhanced whole genome sequence and annotation of Clostridium stercorarium DSM8532T using RNA-Seq transcriptomics and high-throughput proteomics. BMC Genomics 15: 567-583. https://doi.org/10.1186/1471-2164-15-567
  30. Shiratori H, Sasaya K, Ohiwa H, Ikeno H, Ayame S, Kataoka N, et al. 2009. Clostridium clariflavum sp. nov. and Clostridium caenicola sp. nov., moderately thermophilic, cellulose-/cellobiose-digesting bacteria isolated from methanogenic sludge. Int. J. Syst. Evol. Microbiol. 59: 1764-1770.
  31. Hormeyer HF, Tailliez P, Millet J, Girard H, Bonn G, Bobleter O, et al. 1998. Ethanol production by Clostridium thermocellum grown on hydrothermally and organosolvpretreated lignocellulosic materials. Appl. Microbiol. Biotechnol. 29: 528-535.
  32. Leitao V, Noronha EF, Camargo BR, Hamann PRV, Steindorff AS, Quirino BF, et al. 2017. Growth and expression of relevant metabolic genes of Clostridium thermocellum cultured on lignocellulosic residues. J. Ind. Microbiol. Biotechnol. 44: 825-834. https://doi.org/10.1007/s10295-017-1915-2
  33. Espina G, Eley K, Pompidor G, Schneider TR, Crennell SJ, Danson MJ. 2014. A novel ${\beta}$-xylosidase structure from Geobacillus thermoglucosidasius: the first crystal structure of a glycoside hydrolase family GH52 enzyme reveals unpredicted similarity to other glycoside hydrolase folds. Acta Crystallogr. D 70: 1366-1374. https://doi.org/10.1107/S1399004714002788
  34. Lin PP, Rabe KS, Takasumi JL, Kadisch M, Arnold FH, Liao JC. 2014. Isobutanol production at elevated temperatures in thermophilic Geobacillus thermoglucosidasius. Metab. Eng. 24: 1-8. https://doi.org/10.1016/j.ymben.2014.03.006

Cited by

  1. Production of an alkali-stable xylanase from Bacillus pumilus K22 and its application in tomato juice clarification vol.33, pp.4, 2018, https://doi.org/10.1080/08905436.2019.1674157
  2. Digestibility of Wheat and Cattail Biomass Using a Co-culture of Thermophilic Anaerobes for Consolidated Bioprocessing vol.13, pp.1, 2018, https://doi.org/10.1007/s12155-020-10103-0
  3. N-Terminal Fused Signal Peptide Prompted Extracellular Production of a Bacillus-Derived Alkaline and Thermo Stable Xylanase in E. coli Through Cell Autolysis vol.192, pp.2, 2018, https://doi.org/10.1007/s12010-020-03323-9
  4. Symbiotic Bacteroides and Clostridium-rich methanogenic consortium enhanced biogas production of high-solid anaerobic digestion systems vol.14, pp.None, 2021, https://doi.org/10.1016/j.biteb.2021.100685
  5. Metagenomic Insight into Lignocellulose Degradation of the Thermophilic Microbial Consortium TMC7 vol.31, pp.8, 2018, https://doi.org/10.4014/jmb.2106.06015