Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Nematicidal Compounds from the Leaves of Schinus terebinthifolius Against Root-knot Nematode, Meloidogyne incognita Infecting Tomato

  • Abdel Bar, Fatma M. (Department of Pharmacognosy, Faculty of Pharmacy, Prince Sattam Bin Abdulaziz University) ;
  • Ibrahim, Dina S. (Department of Nematology, Plant Pathology Res. Inst., Agric. Res. Center) ;
  • Gedara, Sahar R. (Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University) ;
  • Abdel-Raziq, Mohammed S. (Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University) ;
  • Zaghloul, Ahmed M. (Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University)
  • Received : 2018.05.31
  • Accepted : 2018.08.18
  • Published : 2018.12.31
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Abstract

The root-knot nematode, Meloidogyne incognita caused a serious damage to many plants. The phenolic components of the leaves of Schinus terebinthifolius were investigated as potential nematicidal agents for M. incognita. Nine compounds were isolated and characterized as viz., 1,2,3,4,6-pentagalloyl glucose (1), kaempferol-3-O-${\alpha}$-L-rhamnoside (Afzelin) (2), quercetin-3-O-${\alpha}$-L-rhamnoside (Quercetrin) (3), myricetin (4), myricetin-3-O-${\alpha}$-L-rhamnoside (Myricetrin) (5), methylgallate (6), protocatechuic acid (7), quercetin (8), and gallic acid (9) using nuclear magnetic resonance (NMR) spectroscopy. Compound 1 showed pronounced nematicidal activity compared to Oxamyl as a positive control. It showed the lowest eggs-hatchability (34%) and the highest mortality in nematode population (21% after 72 hours of treatment) at a concentration of $200{\mu}g/mL$. It exhibited the best suppressed total nematode population, root galling and number of eggmasses in infected tomato plants. The total carbohydrates and proteins were also significantly induced by 1 with reduction in total phenolics and increase in defense-related proteins. Thus, compound 1 could be a promising, more safe and effective natural nematicidal agent for the control of root-knot nematodes.

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