Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Efficient Isolation of Dihydrophaseic acid 3'-O-β-D-Glucopyranoside from Nelumbo nucifera Seeds Using High-performance Countercurrent Chromatography and Reverse-phased High-performance Liquid Chromatography

  • Rho, Taewoong (College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea) ;
  • Yoon, Kee Dong (College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea)
  • Received : 2018.07.04
  • Accepted : 2018.08.18
  • Published : 2018.12.31
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Abstract

High-performance countercurrent chromatography (HPCCC) coupled with reversed-phase highperformance liquid chromatography (RP-HPLC) method was developed to isolate dihydrophaseic acid 3'-O-${\beta}$-D-glucopyranoside (DHPAG) from the extract of Nelumbo nucifera seeds. Enriched DHPAG sample (2.3 g) was separated by HPCCC using ethyl acetate/n-butanol/water system (6:4:10, v/v/v, normal-phase mode, flow rate: 4.0 mL/min) to give 23.1 mg of DHPAG with purity of 88.7%. Further preparative RP-HPLC experiment gave pure DHPAG (16.3 mg, purity > 98%). The current study demonstrates that utilization of CCC method maximizes the isolation efficiency compared with that of solid-based conventional column chromatography.

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References

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