Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지
DOI QR코드

DOI QR Code

Quantitative analysis of a myxosporean parasite, Parvicapsula sp. detected from emaciated olive flounder, Paralichthys olivaceus in Korea

국내 여윔 넙치에서 검출된 점액포자충 Parvicapsula sp.의 정량적 분석

  • 김승민 (주식회사 우진비앤지) ;
  • 정준범 (제주대학교 해양의생명과학부)
  • Received : 2018.11.05
  • Accepted : 2018.12.10
  • Published : 2018.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Quantitative analysis of a myxosporean parasite, Parvicapsula sp. in internal organs (kidney, intestine, spleen, brain and liver) from non-emaciated (farm-A) or emaciated (farm-B and farm-C) olive flounder Paralichthys olivaceus were performed by real-time PCR. The highest DNA copy number ($1.7{\times}10^7copies/mg$ tissue) was detected in kidney of the emaciated olive flounder from farm-C, while the DNA copy number was below detection limit in all the organs of the olive flounder from farm-B. There was not positive result in all of organs from olive flounder in farm-A. PCR and histopathological analysis were also performed using the same specimen and showed same results as those by real-time PCR.

여윔증상이 나타난 양식장(farm-B 및 farm-C)의 넙치 및 여윔증상이 나타나지 않은 양식장(farm-A) 넙치의 각 내부 장기(신장, 장, 비장, 뇌 및 간)를 대상으로 점액포자충 Parvicapsula sp.의 양적 분석을 real-time PCR 방법을 사용하여 각각 실시하였다. 여윔증상을 보였던 farm-C의 넙치 신장에서 가장 높은 DNA copy number ($1.7{\times}10^7copies/mg$ tissue)를 보였고, farm-B의 넙치에서는 모든 내부 장기에서 낮은 수치가 나타났으며, farm-A의 넙치에서는 모든 내부 장기에서 음성 결과를 나타내었다. 동일한 시료를 사용한 PCR 및 병리조직학적 분석에서도 real-time PCR에서의 결과와 같은 양상을 보였다.

Keywords

References

  1. Cohen, M., Harrison, P.F. and Lederberg, J. Antimicrobial Resistance: Issues and Options. Division of Health Sciences Policy, pp. 41, 1998.
  2. Ishimatsu, A., Hayashi, M., Nakane, M. and Sameshima, M. Pathophysiology of cultured tiger puffer Takifugu rubripes suffering from the myxosporean emaciation disease. Fish Pathol., 42, 211-217, 2007. https://doi.org/10.3147/jsfp.42.211
  3. Kim, S.M., Jun, L.J., Park, M.A., Jeong, H.D. and Jeong, J.B. Characterization of the myxosporean parasite isolated from emaciated olive flounders Paralichthys olivaceus on Jeju island. Korean J. Fish. Aquat. Sci., 48(3), 337-345, 2015a. https://doi.org/10.5657/KFAS.2015.0337
  4. Kim, S.M., Jun, L.J., Park, M.A., Jung, S.H., Jeong, H.D. and Jeong, J.B. Monitoring of emaciation disease in cultured olive flounder Paralichthys olivaceus in Jeju (2010-2013), Korea. Korean J. Fish. Aquat. Sci., 48(5), 719-724, 2015b. https://doi.org/10.5657/KFAS.2015.0719
  5. Kim, S.M., Jun, L.J., Lee, D.W., Park, H.K., Kim, J.S. and Jeong, J.B. Hematological analysis and non-specific immune responses of emaciated olive flounder, Paralichthys olivaceus in Korea. J. Fish. Mar. Sci. Edu., 29(6), 1758-1767, 2017. https://doi.org/10.13000/JFMSE.2017.29.6.1758
  6. Kim, S.M., Jun, L.J., Lee, D.W., Park, H.K., Jeong, H.D., Kim, J.S. and Jeong, J.B. Monitoring of emaciation disease in culture Paralichthys olivaceus of Jeju island during 2014-2015. Fish. Aquat. Sci., 21(17), 1-7, 2018. https://doi.org/10.1186/s41240-017-0078-4
  7. OIE, International Office of Epizootics. Aquatic Animal Health Standards Commission. Manual of diagnostic tests for Aquatic animals Office international des epizooties, 2018.
  8. Tun, T., Yokoyama, H., Ogawa, K. and Wakabayashi, H. Myxosporeans and their hyperparasitic microsporeans in the intestine of emaciated tiger puffer. J. Fish Pathol. 35, 145-156, 2000. https://doi.org/10.3147/jsfp.35.145
  9. Yanagida, T., Palenzuela, O., Hirae, T., Tanaka, S., Yokoyama, H. and Ogawa, K. Myxosporean emaciation disease of cultured red sea bream Pagrus major and spotted knifejaw Oplegnathus punctatus. Fish Pathol. 43(1), 45-48, 2008. https://doi.org/10.3147/jsfp.43.45
  10. Yasuda, H., Ooyama, T., Nakamura, A., lwata, K., Palenzuela, O. and Yokoyama, H. Occurrence of the myxosporean emaciation disease caused by Enteromyxum leei in cultured Japanese flounder Paralichthys olivaceus. Fish Pathol., 40, 175-180, 2005. https://doi.org/10.3147/jsfp.40.175