Journal of the Korean Society of Marine Environment & Safety (해양환경안전학회지)

The Korean Society of Marine Environment and safety (해양환경안전학회)
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Molecular Phylogeny of Chattonella (Raphidophyceae) Species from Deungnyang Bay, Korea Using Single-Cell PCR

Single-cell PCR을 이용하여 분석한 득량만 Chattonella 종 (Raphidophyceae)의 분자계통학적 특성

  • Kim, Jin Joo (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS)) ;
  • Song, Seon Yeung (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS)) ;
  • Park, Tae Gyu (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS))
  • 김진주 (국립수산과학원 남동해수산연구소) ;
  • 송선영 (국립수산과학원 남동해수산연구소) ;
  • 박태규 (국립수산과학원 남동해수산연구소)
  • Received : 2018.11.07
  • Accepted : 2018.12.28
  • Published : 2018.12.31
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Abstract

The genus Chattonella belonging to the class raphidophyceae, is a harmful algal bloom species. Recently, its occurrence has been increasing and expanding along the Korean coast. Species identification of the genus Chattonella only by morphological observation is difficult due to the lack of rigid cell walls. In this study, the morphological characteristics and genetic affinity of Chattonella sp. isolated from Deungnyang Bay in 2017 were examined. We carried out single-cell isolation from field samples then sequenced three different areas using the single-cell PCR method: 1) parts of ribosomal operon, the large subunit (LSU) of the rDNA, 2) the chloroplast-encoded subunit psaA of Photosystem I, and 3) rbcL encoding the large subunit of the Rubisco gene. The cells were morphologically very similar to the general genus Chattonella ($74.0{\pm}10.1{\mu}m$ in length, $33.1{\pm}3.6{\mu}m$ in width). The three partial gene sequences were insufficient to justify distinction at the species rank. However, they clustered at 99-100 % sequence similarity with C. marina, C. marina var. antiqua and C. marina var. ovata.

최근 우리나라 연안에서 출현빈도가 점차 늘어나고 있는 침편모조류에 속하는 Chattonella는 대표적인 유해조류 중 하나로, 이들 종은 세포벽이 없어, 단순히 세포의 형태나 크기 등 광학현미경 관찰만으로는 정확하게 동정하는 것이 어렵다. 따라서 본 연구에서는 2017년 득량만에서 발생한 Chattonella 적조 시료를 대상으로 단일 세포를 분리하고, 이들 시료의 28s rDNA, rbcL, psaA 영역을 대상으로 single-cell PCR 기법을 이용하여 종 동정을 실시하였다. 현미경 관찰 결과 장축은 평균 $74.0{\pm}10.1{\mu}m$이고 단축은 평균 $33.1{\pm}3.6{\mu}m$로 일반적인 Chattonella의 형태적 특징을 보였다. 28s rDNA, rbcL, psaA 영역을 대상으로 한 염기서열 비교 결과에서는 세 영역 모두에서 하나의 종으로 명확히 구분되지는 않았다. 하지만 C. marina, C. marina var. antiqua, C. marina var. ovata 그룹과 99~100 % 높은 서열 유사성을 보였다.

Keywords

References

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