Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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In-vitro antioxidant activity of flavonoids from Acer okamotoanum

  • Kim, Ji Hyun (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University) ;
  • Kim, Hyun Young (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University)
  • Received : 2018.04.26
  • Accepted : 2018.09.18
  • Published : 2018.12.31
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Abstract

Degenerative diseases are commonly associated with excess free radicals. Acer okamotoanum, a plant endemic to Korea, is reported to have anti-oxidant, anti-cancer, and anti-viral activities. We previously isolated flavonoids from the ethyl acetate fraction of A. okamotoanum such as quercitrin (QU), isoquercitrin (IQ), and afzelin (AF). In the present study, the in vitro antioxidant activity of flavonoids such as QU, IQ, and AF isolated from the ethyl acetate fraction of A. okamotoanum were investigated by measuring the free radical scavenging activity including 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical ($^{\cdot}OH$), and superoxide anion ($O_2{^-}$). The flavonoids (QU, IQ, and AF) concentration-dependently showed a DPPH radical scavenging activity. In particular, QU and IQ showed a higher DPPH radical scavenging activity than that of AF. In addition, the flavonoids (QU, IQ, and AF) at $10{\mu}g/mL$ showed over an 80% scavenging effect against $^{\cdot}OH$ radical production. Furthermore, the $O_2{^-}$ radical scavenging activity of the flavonoids, QU, IQ, and AF increased in a dose-dependent manner. Particularly, IQ exerted the strongest scavenging activities against $^{\cdot}OH$ and $O_2{^-}$ radicals among the other flavonoids. These results indicate that the flavonoids from A. okamotoanum, in particular IQ, would have a protective activity against oxidative stress induced by free radicals, and potentially be considered as a natural antioxidant agent.

Keywords

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Fig. 1. The structures of favonoids from Acer okamotoanum. QU, quercitrin; IQ, isoquercitrin; AF, afzelin; O-Rham, O-Rhamnoside; O-Glc, O-glucoside.

Table 1. DPPH radical scavenging activity of favonoids from Acer okamotoanum.

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Table 2. Hydroxyl radical (•OH) scavenging activity of favonoids from Acer okamotoanum.

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Table 3. Superoxide anion (O2-) scavenging activity of favonoids from Acer okamotoanum.

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