Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Hepatoprotective Effect of Solvent Fractions from Raphiolepis indica against Oxidative Stress

  • Kim, Sun Young (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Kim, Hye-Ran (Department of Clinical Laboratory Science, College of Medical Sciences, Daegu Haany University) ;
  • Chang, Kyung-Soo (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
  • Received : 2018.11.02
  • Accepted : 2018.12.17
  • Published : 2018.12.31
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Abstract

Raphiolepis indica (R. indica) is one of evergreen shrubs belonging to the Rosaceae and is grown wildly in Jeju. This study was performed to evaluate the hepatoprotective effect of different fractions (n-hexane, dichloromethane, ethyl acetate, butanol, water) from R. indica. Anti-oxidative effects were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and total phenol contents. Hepatoprotective effect was identified by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay in Huh7 cells. Among various fractions, ethyl acetate and butanol fractions showed the lowest DPPH remained rate reaching approximately 78.7 and 65.5% at $400{\mu}g/mL$. Ethyl acetate and butanol fractions showed the total phenolic content at 164.5 and 137.3 mg GAE/g extract. The ethyl acetate and butanol fractions were resistant against oxidative stress in MTT assay and showed higher hepatoprotective effect than other fractions. Therefore, these results suggest that the ethyl acetate and butanol fractions of R. indica might have therapeutic value in liver damage.

Keywords

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Fig. 1. DPPH radical remained rate of Solvent Fractions from Raphiolepis indica and quercetin. Abbreviation: HF, hexane fraction; MF, methylene chloride fraction; EF, ethyl acetate fraction; BF, butanol fraction; DW, DW fraction; QUE, quercetin.

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Fig. 2. Cell viability on Huh7 cells. (A) hexane fraction, (B) dichloromethane fraction, (C) ethyl acetate fraction, (D) butanol fraction, (E) water fraction, (F) quercetin

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Fig. 3. Cytoprotective effects on Huh7 cells against hydrogen peroxide-induced oxidative damage. Abbreviation: EtOAc, ethyl acetate fraction; BuOH, butanol fraction.

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Fig. 4. Cytoprotective effects on Huh7 cells against tert-butyl hydroperoxide -induced oxidative damage. Abbreviation: EtOAc, ethyl acetate fraction; BuOH, buthanol fraction.

Table 1. Total phenolic content of solvent fractions from Raphiolepis indica

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