The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Immune regulation effects of Gentianae Radix extract in LPS-induced acute inflammatory mice

LPS로 급성 염증을 유발한 동물에 대한 용담초 추출물의 면역조절 효과

  • Lee, Hyo-Jung (Department of Beauty and Healthy medicine, Daejeon University) ;
  • Seung, Yoon-Cheol (Department of Oriental Health Care, Joongbu University) ;
  • Lee, Myung-Sun (Department of Beauty and Healthy medicine, Daejeon University)
  • 이효정 (대전대학교 자연과학대학 뷰티건강관리학과) ;
  • 승윤철 (중부대학교 한방건강관리학과) ;
  • 이명선 (대전대학교 자연과학대학 뷰티건강관리학과)
  • Received : 2018.02.09
  • Accepted : 2018.03.15
  • Published : 2018.03.30
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Abstract

Objective : The immune enhance is the main focus of current society that to increase resistance to invasion by pathogenic species of bacteria in body, stimulate the immune system and possibly protect against cancer or inflammatory disease. The present study aimed to evaluate the effect of Gentianae Radix extract on immune regulation in a LPS-induced mice model of acute inflammation. Methods : Gentianae Radix extract was administered orally at doses of 200 mg/kg/day or 400 mg/kg/day for 2 weeks before a intraperitoneally injection of LPS (1 mg/kg of 0.9% saline). After LPS-intraperitoneal injection 3 hours, blood was collected by cardiac puncture under ether anaesthesia from all animals, for the immune regulate efficacy verification based on blood or serum biomarkers (i.e., immune cells, cytokine, $PGE_2$, ROS, and $LTB_4$) analysis. Results : Compared to the control mice, the Gentianae Radix extract treatments significantly increased the count of immune cells (i.e., wite blood cell, neutrophils, and monocyte), and significantly reduced the lymphocyte. In addition, the Gentianae Radix extract treatments significantly decreased the pro-inflammatory cytokine (i.e., $IL-1{\beta}$, IL-6, and $TNF-{\alpha}$), and significantly increased IL-10 of anti-inflammatory cytokine. Furthermore, the Gentianae Radix extracts treatments significantly increased the levels of $PGE_2$ and significantly decreased the levels of ROS, and $LTB_4$. Conclusions : The results indicate that Gentianae Radix extract alleviated acute inflammatory reaction though regulation of immune meditor. Thus, Gentianae Radix extract may raw material of development a health food and medicine option for the immune enhance.

Keywords

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