Environmental Engineering Research

Korean Society of Environmental Engineers (대한환경공학회)
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A combined approach to evaluate activity and structure of soil microbial community in long-term heavy metals contaminated soils

  • Wang, Tianqi (School of Energy & Environmental Engineering, and National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing) ;
  • Yuan, Zhimin (School of Energy & Environmental Engineering, and National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing) ;
  • Yao, Jun (School of Energy & Environmental Engineering, and National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing)
  • Received : 2017.05.17
  • Accepted : 2017.08.23
  • Published : 2018.03.31
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Abstract

In the present study, long-term heavy metals (HMs) contaminated soil samples from a well-known Pb/Zn smelting area in the southwest of China were collected, and physicochemical and biological characteristics of these samples were evaluated. Soil samples contained different concentrations of HMs, namely Pb, Zn, Cu, and Cd. Enzyme activity analyses combined with microcalorimetric analysis were used for soil microbial activity evaluation. Results showed that two soil samples, containing almost the highest concentrations of HMs, also shared the greatest microbial activities. Based on correlation coefficient analysis, high microbial activity in heavily HMs contaminated soil might be due to the high contents of soil organic matter and available phosphorus in these samples. High-throughput sequencing technique was used for microbial community structure analysis. High abundance of genera Sphingomonas and Thiobacillus were also observed in these two heavily contaminated soils, suggesting that bacteria belonging to these two genera might be further isolated from these contaminated soils and applied for future studies of HMs remediation. Results of present study would contribute to the evaluation of microbial communities and isolation of microbial resources to remediate HMs pollution.

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References

  1. Coppolecchia D, Puglisi E, Vasileiadis S, et al. Relative sensitivity of different soil biological properties to zinc. Soil Biol. Biochem. 2011;43:1798-1807. https://doi.org/10.1016/j.soilbio.2010.06.018
  2. Li ZY, Ma ZW, van der Kuijp TJ, Yuan ZW, Huang L. A review of soil heavy metal pollution from mines in China: Pollution and health risk assessment. Sci. Total Environ. 2014;468-469:843-853. https://doi.org/10.1016/j.scitotenv.2013.08.090
  3. Li J, Ma YB, Hu HW, Wang JT, Liu YR, He JZ. Field-based evidence for consistent responses of bacterial communities to copper contamination in two contrasting agricultural soils. Front. Microbiol. 2015;6:31.
  4. Bi XY, Feng XB, Yang YG, et al. Environmental contamination of heavy metals from zinc smelting areas in Hezhang County, western Guizhou, China. Environ. Int. 2006;32:883-890. https://doi.org/10.1016/j.envint.2006.05.010
  5. Yuan Z, Yao J, Wang F, et al. Potentially toxic trace element contamination, sources, and pollution assessment in farmlands, Bijie City, southwestern China. Environ. Monit. Assess. 2017;189:25. https://doi.org/10.1007/s10661-016-5755-8
  6. Zhao Y, Yao J, Yuan ZM, Wang TQ, Zhang YY, Wang F. Bioremediation of Cd by strain GZ-22 isolated from mine soil based on biosorption and microbially induced carbonate precipitation. Environ. Sci. Pollut. Res. 2017;24:372-380. https://doi.org/10.1007/s11356-016-7810-y
  7. Velmurugan P, Shim J, You Y, et al. Removal of zinc by live, dead, and dried biomass of Fusarium spp. isolated from the abandoned-metal mine in South Korea and its perspective of producing nanocrystals. J. Hazard. Mater. 2010;182:317-324. https://doi.org/10.1016/j.jhazmat.2010.06.032
  8. del Carmen Vargas-Garcia M, Jose Lopez M, Suarez-Estrella F, Moreno J. Compost as a source of microbial isolates for the bioremediation of heavy metals: In vitro selection. Sci. Total. Environ. 2012;431:62-67. https://doi.org/10.1016/j.scitotenv.2012.05.026
  9. Naik MM, Dubey SK. Lead resistant bacteria: Lead resistance mechanisms, their applications in lead bioremediation and biomonitoring. Ecotox. Environ. Safe. 2013;98:1-7. https://doi.org/10.1016/j.ecoenv.2013.09.039
  10. Subrahmanyam G, Shen JP, Liu YR, Archana G, Zhang LM. Effect of long-term industrial waste effluent pollution on soil enzyme activities and bacterial community composition. Environ. Monit. Assess. 2016;188:112.
  11. Garcia-Gil JC, Kobza J, Soler-Rovira P, Javorekova S. Soil microbial and enzyme activities response to pollution near an aluminium smelter. Clean-Soil Air Water. 2013;41:485-492. https://doi.org/10.1002/clen.201200099
  12. Azarbad H, Niklinska M, Laskowski R, et al. Microbial community composition and functions are resilient to metal pollution along two forest soil gradients. FEMS Microbiol. Ecol. 2015;91:1-11.
  13. Niemeyer JC, Lolata GB, de Carvalho GM, Da Silva EM, Sousa JP, Nogueira MA. Microbial indicators of soil health as tools for ecological risk assessment of a metal contaminated site in Brazil. Appl. Soil Ecol. 2012;59:96-105. https://doi.org/10.1016/j.apsoil.2012.03.019
  14. Azarbad H, Niklinska M, van Gestel CAM, van Straalen NM, Roling WFM, Laskowski R. Microbial community structure and functioning along metal pollution gradients. Environ. Toxicol. Chem. 2013;32:1992-2002. https://doi.org/10.1002/etc.2269
  15. Zhang FP, Li CF, Tong LG, et al. Response of microbial characteristics to heavy metal pollution of mining soils in central Tibet, China. Appl. Soil Ecol. 2010;45:144-151. https://doi.org/10.1016/j.apsoil.2010.03.006
  16. Green V, Stott D, Diack M. Assay for fluorescein diacetate hydrolytic activity: Optimization for soil samples. Soil Biol. Biochem. 2006;38:693-701. https://doi.org/10.1016/j.soilbio.2005.06.020
  17. Guo H, Yao J, Cai MM, et al. Effects of petroleum contamination on soil microbial numbers, metabolic activity and urease activity. Chemosphere 2012;87:1273-1280. https://doi.org/10.1016/j.chemosphere.2012.01.034
  18. Ge TD, Nie SA, Wu JS, et al. Chemical properties, microbial biomass, and activity differ between soils of organic and conventional horticultural systems under greenhouse and open field management: A case study. J. Soil Sediment. 2010;11:25-36.
  19. Chen HL, Yao J, Wang F, et al. Toxicity of three phenolic compounds and their mixtures on the gram-positive bacteria Bacillus subtilis in the aquatic environment. Sci. Total Environ. 2010;408:1043-1049. https://doi.org/10.1016/j.scitotenv.2009.11.051
  20. Wang F, Yao J, Chen HL, Chen K, Trebse P, Zaray G. Comparative toxicity of chlorpyrifos and its oxon derivatives to soil microbial activity by combined methods. Chemosphere 2010;78:319-326. https://doi.org/10.1016/j.chemosphere.2009.10.030
  21. Hong C, Si YX, Xing Y, Li Y. Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas. Environ. Sci. Pollut. Res. 2015;22:10788-10799. https://doi.org/10.1007/s11356-015-4186-3
  22. Kuppusamy S, Thavamani P, Megharaj M, Venkateswarlu K, Lee YB, Naidu R. Pyrosequencing analysis of bacterial diversity in soils contaminated long-term with PAHs and heavy metals: Implications to bioremediation. J. Hazard. Mater. 2016;317: 169-179. https://doi.org/10.1016/j.jhazmat.2016.05.066
  23. Xun WB, Huang T, Zhao J, et al. Environmental conditions rather than microbial inoculum composition determine the bacterial composition, microbial biomass and enzymatic activity of reconstructed soil microbial communities. Soil Biol. Biochem. 2015;90:10-18. https://doi.org/10.1016/j.soilbio.2015.07.018
  24. Bremner J, Sparks D, Page A, et al. Nitrogen-total. In: Sparks D, Page A, et al., eds. Methods of Soil Analysis Part 3 - Chemical Methods, SSSA Book Series 5.3. 1996. p. 1085-1121.
  25. Wang CH, Gu ZH, Cui H, Zhu HH, Fu SL, Yao Q. Differences in Arbuscular Mycorrhizal Fungal community composition in soils of three land use types in subtropical hilly area of Southern China. PLoS One. 2015;10:e0130983. https://doi.org/10.1371/journal.pone.0130983
  26. Jones DL, Simfukwe P, Hill PW, Mills RTE, Emmett BA. Evaluation of dissolved organic carbon as a soil quality indicator in national monitoring schemes. PLoS One. 2014;9:e90882. https://doi.org/10.1371/journal.pone.0090882
  27. Zandstra H, MacKenzie A. Potassium exchange equilibria and yield responses of oats, barley, and corn on selected Quebec soils. Soil Sci. Soc. Am. J. 1968;32:76-79. https://doi.org/10.2136/sssaj1968.03615995003200010019x
  28. Lee SS, Lim JE, El-Azeem SAA, et al. Heavy metal immobilization in soil near abandoned mines using eggshell waste and rapeseed residue. Environ. Sci. Pollut. Res. 2013;20:1719-1726. https://doi.org/10.1007/s11356-012-1104-9
  29. Yuan ZM, Zhao Y, Guo ZW, Yao J. Chemical and ecotoxicological assessment of multiple heavy metal-contaminated soil treated by phosphate addition. Water Air Soil Pollut. 2016;227:403.
  30. Chen HL, Yao J, Wang F, et al. Investigation of the acute toxic effect of chlorpyrifos on Pseudomonas putida in a sterilized soil environment monitored by microcalorimetry. Arch. Environ. Con. Tox. 2010;58:587-593. https://doi.org/10.1007/s00244-009-9404-x
  31. Zhang W, Chen L, Zhang R, Lin KF. High throughput sequencing analysis of the joint effects of BDE209-Pb on soil bacterial community structure. J. Hazard. Mater. 2016;301:1-7.
  32. Mondal NK, Dey U, Ghosh S, Datta JK. Soil enzyme activity under arsenic-stressed area of Purbasthali, West Bengal, India. Arch. Agron. Soil Sci. 2015;61:73-87. https://doi.org/10.1080/03650340.2014.922178
  33. Niu FJ, He JX, Zhang GS, et al. Effects of enhanced UV-B radiation on the diversity and activity of soil microorganism of alpine meadow ecosystem in Qinghai-Tibet Plateau. Ecotoxicology 2014;23:1833-1841. https://doi.org/10.1007/s10646-014-1314-7
  34. Mignardi S, Corami A, Ferrini V. Evaluation of the effectiveness of phosphate treatment for the remediation of mine waste soils contaminated with Cd, Cu, Pb, and Zn. Chemosphere 2012;86:354-360. https://doi.org/10.1016/j.chemosphere.2011.09.050
  35. Osborne LR, Baker LL, Strawn DG. Lead immobilization and phosphorus availability in phosphate-amended, mine-contaminated soils. J. Environ. Qual. 2015;44:183-190. https://doi.org/10.2134/jeq2014.07.0323
  36. Cao XD, Wahbi A, Ma LN, Li B, Yang YL. Immobilization of Zn, Cu, and Pb in contaminated soils using phosphate rock and phosphoric acid. J. Hazard. Mater. 2009;164:555-564. https://doi.org/10.1016/j.jhazmat.2008.08.034
  37. Masakorala K, Yao J, Chandankere R, et al. A combined approach of physicochemical and biological methods for the characterization of petroleum hydrocarbon-contaminated soil. Environ. Sci. Pollut. Res. 2014;21:454-463. https://doi.org/10.1007/s11356-013-1923-3
  38. Mejias Carpio IE, Franco DC, Zanoli Sato MI, et al. Biostimulation of metal-resistant microbial consortium to remove zinc from contaminated environments. Sci. Total Environ. 2016;550:670-675. https://doi.org/10.1016/j.scitotenv.2016.01.149
  39. Ho L, Hoefel D, Saint CP, Newcombe G. Isolation and identification of a novel microcystin-degrading bacterium from a biological sand filter. Water Res. 2007;41:4685-4695. https://doi.org/10.1016/j.watres.2007.06.057
  40. Tangaromsuk J, Pokethitiyook P, Kruatrachue M, Upatham ES. Cadmium biosorption by Sphingomonas paucimobilis biomass. Bioresour. Technol. 2002;85:103-105. https://doi.org/10.1016/S0960-8524(02)00066-4
  41. Quadros PDd, Zhalnina K, Davis-Richardson AG, et al. Coal mining practices reduce the microbial biomass, richness and diversity of soil. Appl. Soil Ecol. 2016;98:195-203. https://doi.org/10.1016/j.apsoil.2015.10.016
  42. Lombardi AT, Garcia Jr O. Biological leaching of Mn, Al, Zn, Cu and Ti in an anaerobic sewage sludge effectuated by Thiobacillus ferrooxidans and its effect on metal partitioning. Water Res. 2002;36:3193-3202. https://doi.org/10.1016/S0043-1354(02)00008-8
  43. Kai T, Suenaga Y-i, Migita A, Takahashi T. Kinetic model for simultaneous leaching of zinc sulfide and manganese dioxide in the presence of iron-oxidizing bacteria. Chem. Eng. Sci. 2000;55:3429-3436. https://doi.org/10.1016/S0009-2509(00)00014-2

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