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Anti-Pigmentation Effects of Eight Phellinus linteus-Fermented Traditional Crude Herbal Extracts on Brown Guinea Pigs of Ultraviolet B-Induced Hyperpigmentation

  • Ahn, Hee-Young (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Choo, Young-Moo (Jeonju AgroBio-Materials Institute) ;
  • Cho, Young-Su (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
  • Received : 2017.11.24
  • Accepted : 2017.12.12
  • Published : 2018.03.28

Abstract

We have previously found that mycelia culture broth of eight kinds of traditional herbal extracts fermented with Phellinus linteus (previously named as 8-HsPLCB) not only inhibited melanin and tyrosinase activity, but also reduced the contents of melanogenesis-related proteins, including tyrosinase and microphthalmia-associated transcription factor, in 3-isobutyl-1-methylxanthine-stimulated B16F0 melanoma cells. For a further study, the effect of 8-HsPLCB against skin pigmentation in brown guinea pigs with ultraviolet B (UVB)-induced hyperpigmentation was investigated. 8-HsPLCB (3%) and arbutin (2%) as positive controls were applied topically twice daily for 4 weeks to the hyperpigmented areas. 8-HsPLCB showed skin-lightening effect as effective as arbutin, one of the most widely used in whitening cosmetics. Melanin index values as the degree of pigmentation showed a significant reduction week by week post 8-HsPLCB treatment and then substantially reduced by 4 weeks. The degree of depigmentation after 4 weeks of topical application with 8-HsPLCB was 32.2% as compared with before treatment (0 week). Moreover, using Fontana-Masson staining and hematoxylin-eosin staining, 8-HsPLCB reduced melanin pigmentation in the basal layer of the epidermis and epidermal thickness changes exposed to the UV-B irradiation as compared with non-treatment and vehicle treatment. The intensity of the skin-lightening effect of 8-HsPLCB was similar to arbutin. These results suggest that the skin-lightening effect of 8-HsPLCB might be resulted from inhibition of melanin synthesis by tyrosinase in melanocytes. To conclude, 8-HsPLCB treatment showed reduction of the melanin pigment and histological changes induced by UV irradiation in brown guinea pigs.

Keywords

References

  1. Brenner M, Berking C. 2010. Principles of skin pigmentation. Biochemistry and regulation of melanogenesis. Hautarzt 61: 554-560.
  2. Taylor SC. 2003. Epidemiology of skin diseases in people of color. Cutis 71: 271-275.
  3. Mishima Y. 1994. Molecular and biological control of melanogenesis through tyrosinase genes and intrinsic and extrinsic regulatory factors. Pigment Cell Res. 7: 376-387. https://doi.org/10.1111/j.1600-0749.1994.tb00065.x
  4. Bradford MM. 1976. A r apid and s ensitive m ethod for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  5. Ishikawa M, K awase I, I shii F. 2007. Glycine i nhibits melanogenesis in vitro and causes hypopigmentation in vivo. Biol. Pharm. Bull. 30: 2031-2036. https://doi.org/10.1248/bpb.30.2031
  6. Lee KT, Kim BJ, Kim JH, Heo MY, Kim HP. 1997. Biological screening of 100 plant extracts for cosmetic use (I): inhibitory activities of tyrosinase and DOPA auto-oxidation. Int. J. Cosmet. Sci. 19: 291-298. https://doi.org/10.1111/j.1467-2494.1997.tb00193.x
  7. Zhong S, Wu Y, Ahn SM, Zhao J, Wang K, Yang S, et al. 2006. Depigmentation of melanocytes by the treatment of extracts from traditional Chinese herbs: a cell culture assay. Biol. Pharm. Bull. 29: 1947-1951. https://doi.org/10.1248/bpb.29.1947
  8. Hwang JH, Lee BM. 2007. Inhibitory effects of plant extracts on tyrosinase, L-DOPA oxidation, and melanin synthesis. J. Toxicol. Environ. Health A 70: 393-407. https://doi.org/10.1080/10937400600882871
  9. W ang KH, Lin RD, Hsu FL, Huang YH, Chang HC, Huang CY, et al. 2006. Cosmetic applications of selected traditional Chinese herbal medicines. J. Ethnopharmacol. 106: 353-359. https://doi.org/10.1016/j.jep.2006.01.010
  10. Ch a JY, Yang HJ, Jeong JJ, Seo WS, Park JS, Ok, M, et al. 2010. Tyrosinase inhibition activity and antioxidant capacity by fermented products of some medicinal plants. J. Life Sci. 20: 940-947.
  11. Heo JS, Cha JY, Kim HW, Ahn HY, Eom KE, Heo SJ, et al. 2010. Bioactive materials and biological activity in the extracts of leaf, stem mixture and root from Angelica gigas Nakai. J. Life Sci. 20: 750-759. https://doi.org/10.5352/JLS.2010.20.5.750
  12. Cha JY, Kim HW, Heo JS, Ahn HY, Eom KE, Heo SJ, et al. 2010. Ingredients analysis and biological activity of fermented Angelica gigas Nakai by mold. J. Life Sci. 20: 1385-1393. https://doi.org/10.5352/JLS.2010.20.9.1385
  13. Shon MY. 2007. Antioxidant and a nticancer activities of Poria cocos and Machilus thunbergii fermented with mycelial mushrooms. Food Ind. Nutr. 12: 51-57.
  14. Kwak YJ, Kim KS, Kim KM, Yu HY, Chung E, Kim SJ, et al. 2011. Fermented Viola mandshurica inhibits melanogenesis in B16 melanoma cells. Biosci. Biotechnol. Biochem. 75: 841-847 https://doi.org/10.1271/bbb.100641
  15. Cha JY, Yang HJ, Moon HI, Cho YS. 2012. Inhibitory effect and mechanism on melanogenesis from fermented herbal composition for medical or food uses. Food Res. Int. 45: 225-231. https://doi.org/10.1016/j.foodres.2011.11.002
  16. Tobiishi M, Haratake A, Kaminaga H, Nakahara M, Komiya A, Koishikawa H, et al. 2004. Pigmentation in intrinsically aged skin of A1 guinea pigs. Pigment Cell Res. 17: 651-658. https://doi.org/10.1111/j.1600-0749.2004.00183.x
  17. Kong YH, Jo YO, Cho CW, Son D, Park S, Rho J, et al. 2008. Inhibitory effects of cinnamic acid on melanin biosynthesis in skin. Biol. Pharm. Bull. 31: 946-948.
  18. Verkruysse W, Svaasand LO, Franco W, Nelson JS. 2009. Remittance at a single wavelength of 390 nm to quantify epidermal melanin concentration. J. Biomed. Opt. 14: 1400-1405.
  19. Sticher O, Soldati F, Lehmann D. 1979. High-performance liquid chromatographic separation and quantitative determination of arbutin, methylarbutin, hydroquinone and hydroquinone-monomethylether in Arctostaphylos, Bergenia, Calluna and Vaccinium species (author's transl). Planta Med. 35: 253-261. https://doi.org/10.1055/s-0028-1097213
  20. Lim YJ, Lee EH, Kang TH, Ha SK, Oh MS, Kim SM, et al. 2009. Inhibitory effects of arbutin on melanin biosynthesis of alpha-melanocyte stimulating hormone-induced hyperpigmentation in cultured brownish guinea pig skin tissues. Arch. Pharm. Res. 32: 367-373. https://doi.org/10.1007/s12272-009-1309-8
  21. Dudonne S, Poupard P, Coutiere P, Woillez M, Richard T, Merillon JM, et al. 2011. Phenolic composition and antioxidant properties of Poplar Bud (Populus nigra) extract: individual antioxidant contribution of phenolics and transcriptional effect on skin aging. J. Agric. Food Chem. 59: 4527-4536. https://doi.org/10.1021/jf104791t

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