Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Exploring the Metabolomic Responses of Bacillus licheniformis to Temperature Stress by Gas Chromatography/Mass Spectrometry

  • Dong, Zixing (College of Chemical Engineering and Materials Science, Tianjin University of Science & Technology) ;
  • Chen, Xiaoling (Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science & Technology) ;
  • Cai, Ke (Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science & Technology) ;
  • Chen, Zhixin (Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science & Technology) ;
  • Wang, Hongbin (Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science & Technology) ;
  • Jin, Peng (College of Chemical Engineering and Materials Science, Tianjin University of Science & Technology) ;
  • Liu, Xiaoguang (College of Chemical Engineering and Materials Science, Tianjin University of Science & Technology) ;
  • Permaul, Kugenthiren (Department of Biotechnology & Food Technology, Faculty of Applied Sciences, Durban University of Technology) ;
  • Singh, Suren (Department of Biotechnology & Food Technology, Faculty of Applied Sciences, Durban University of Technology) ;
  • Wang, Zhengxiang (College of Chemical Engineering and Materials Science, Tianjin University of Science & Technology)
  • Received : 2017.08.09
  • Accepted : 2017.12.04
  • Published : 2018.03.28
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Abstract

Owing to its high protein secretion capacity, simple nutritional requirements, and GRAS (generally regarded as safe) status, Bacillus licheniformis is widely used as a host for the industrial production of enzymes, antibiotics, and peptides. However, as compared with its close relative Bacillus subtilis, little is known about the physiology and stress responses of B. licheniformis. To explore its temperature-stress metabolome, B. licheniformis strains ATCC 14580 and B186, with respective optimal growth temperatures of $42^{\circ}C$ and $50^{\circ}C$, were cultured at $42^{\circ}C$, $50^{\circ}C$, and $60^{\circ}C$ and their corresponding metabolic profiles were determined by gas chromatography/mass spectrometry and multivariate statistical analyses. It was found that with increased growth temperatures, the two B. licheniformis strains displayed elevated cellular levels of proline, glutamate, lysine, pentadecanoic acid, hexadecanoic acid, heptadecanoic acid, and octadecanoic acid, and decreased levels of glutamine and octadecenoic acid. Regulation of amino acid and fatty acid metabolism is likely to be associated with the evolution of protective biochemical mechanisms of B. licheniformis. Our results will help to optimize the industrial use of B. licheniformis and other important Bacillus species.

Keywords

References

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