Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지
DOI QR코드

DOI QR Code

Flow cytometric immunophenotyping of canine adipose-derived mesenchymal stem cells (ADMSCs) and feline ADMSCs using anti-human antibodies

  • Ko, Minho (Department of Veterinary Internal Medicine and Institute of Veterinary Science, College of Veterinary Medicine, Kangwon National University) ;
  • Lee, Kwon Young (Department of Veterinary Anatomy, College of Veterinary Medicine, Kangwon National University) ;
  • Kim, Sae Hoon (Department of Orthopedic Surgery, College of Medicine, Seoul National University Hospital) ;
  • Kim, Manho (Department of Neurology, College of Medicine, Seoul National University Hospital) ;
  • Choi, Jung Hoon (Department of Veterinary Internal Medicine and Institute of Veterinary Science, College of Veterinary Medicine, Kangwon National University) ;
  • Im, Wooseok (Department of Neurology, College of Medicine, Seoul National University Hospital) ;
  • Chung, Jin Young (Department of Veterinary Internal Medicine and Institute of Veterinary Science, College of Veterinary Medicine, Kangwon National University)
  • Received : 2018.02.21
  • Accepted : 2018.03.22
  • Published : 2018.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Various trials have been conducted to develop therapies for serious untreatable diseases. Among these, those using stem cells have shown great promise, and adipose-derived mesenchymal stem cells (ADMSCs) are easier to obtain than other types of stem cells. Prior to clinical trials, characterization of ADMSCs with monoclonal antibodies should be performed. However, it is difficult to use species-specific antibodies for veterinarians. This study was conducted to confirm the panel of human antibodies applicable for use in immunophenotypic characterization of canine adipose-derived stem cells and feline ADMSCs extracted from subcutaneous adipose tissue collected during ovariohysterectomy. For flow cytometric immunophenotyping, the third passages of canine ADMSC and feline ADMSC and human CD31, CD34, CD42, CD44, CD62 and CD133 antibodies were used. Of these, CD133 reacted with canine cells (3.74%) and feline cells (1.34%). CD133 is known as a marker related with more primitive stem cell phenotype than other CD series. Because this human CD133 was not a species-specific antibody, accurate percentages of immunoreactivity were not confirmed. Nevertheless, the results of this study confirmed human CD133 as a meaningful marker in canine and feline ADMSCs.

Keywords

References

  1. Arnhold S, Wenisch S. Adipose tissue derived mesenchymal stem cells for musculoskeletal repair in veterinary medicine. Am J Stem Cells 2015, 4, 1-12.
  2. Baer PC. Adipose-derived mesenchymal stromal/stem cells: an update on their phenotype in vivo and in vitro. World J Stem Cells 2014, 6, 256-265. https://doi.org/10.4252/wjsc.v6.i3.256
  3. Bertassoli BM, de Assis Neto AC, de Oliveira FD, Arroyo MAM, Ferrao JSP, da Silva JB, Pignatari GC, Braga PB. Mesenchymal stem cells - emphasis in adipose tissue. Braz Arch Biol Technol 2013, 56, 607-618. https://doi.org/10.1590/S1516-89132013000400011
  4. Bourin P, Bunnell BA, Casteilla L, Dominici M, Katz AJ, March KL, Redl H, Rubin JP, Yoshimura K, Gimble JM. Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). Cytotherapy 2013, 15, 641-648. https://doi.org/10.1016/j.jcyt.2013.02.006
  5. Carrade DD, Borjesson DL. Immunomodulation by mesenchymal stem cells in veterinary species. Comp Med 2013, 63, 207-217.
  6. Handgretinger R, Gordon PR, Leimig T, Chen X, Buhring HJ, Niethammer D, Kuci S. Biology and plasticity of $CD133^{+}$ hematopoietic stem cells. Ann N Y Acad Sci 2003, 996, 141-151. https://doi.org/10.1111/j.1749-6632.2003.tb03242.x
  7. Kono S, Kazama T, Kano K, Harada K, Uechi M, Matsumoto T. Phenotypic and functional properties of feline dedifferentiated fat cells and adipose-derived stem cells. Vet J 2014, 199, 88-96. https://doi.org/10.1016/j.tvjl.2013.10.033
  8. Lim MH, Ong WK, Sugii S. The current landscape of adipose-derived stem cells in clinical applications. Expert Rev Mol Med 2014, 16, e8. https://doi.org/10.1017/erm.2014.8
  9. Marx C, Silveira MD, Beyer Nardi N. Adipose-derived stem cells in veterinary medicine: characterization and therapeutic applications. Stem Cells Dev 2015, 24, 803-813. https://doi.org/10.1089/scd.2014.0407
  10. Parys M, Nelson N, Koehl K, Miller R, Kaneene JB, Kruger JM, Yuzbasiyan-Gurkan V. Safety of intraperitoneal injection of adipose tissue-derived autologous mesenchymal stem cells in cats. J Vet Intern Med 2016, 30, 157-163. https://doi.org/10.1111/jvim.13655
  11. Pascucci L, Curina G, Mercati F, Marini C, Dall'Aglio C, Paternesi B, Ceccarelli P. Flow cytometric characterization of culture expanded multipotent mesenchymal stromal cells (MSCs) from horse adipose tissue: towards the definition of minimal stemness criteria. Vet Immunol Immunopathol 2011, 144, 499-506. https://doi.org/10.1016/j.vetimm.2011.07.017
  12. Penny J, Harris P, Shakesheff KM, Mobasheri A. The biology of equine mesenchymal stem cells: phenotypic characterization, cell surface markers and multilineage differentiation. Front Biosci (Landmark Ed) 2012, 17, 892-908. https://doi.org/10.2741/3963
  13. Ranera B, Lyahyai J, Romero A, Vazquez FJ, Remacha AR, Bernal ML, Zaragoza P, Rodellar C, Martin-Burriel I. Immunophenotype and gene expression profiles of cell surface markers of mesenchymal stem cells derived from equine bone marrow and adipose tissue. Vet Immunol Immunopathol 2011, 144, 147-154. https://doi.org/10.1016/j.vetimm.2011.06.033
  14. Screven R, Kenyon E, Myers MJ, Yancy HF, Skasko M, Boxer L, Bigley EC 3rd, Borjesson DL, Zhu M. Immunophenotype and gene expression profile of mesenchymal stem cells derived from canine adipose tissue and bone marrow. Vet Immunol Immunopathol 2014, 161, 21-31. https://doi.org/10.1016/j.vetimm.2014.06.002
  15. Trzil JE, Masseau I, Webb TL, Chang CH, Dodam JR, Liu H, Quimby JM, Dow SW, Reinero CR. Intravenous adipose-derived mesenchymal stem cell therapy for the treatment of feline asthma: a pilot study. J Feline Med Surg 2016, 18, 981-990. https://doi.org/10.1177/1098612X15604351