Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Morphological and Molecular Characterization of Toxocara tanuki (Nematoda: Ascaridae) from Korean Raccoon Dog, Nyctereutes procyonoides koreensis

  • Alexander, Umanets (College of Veterinary Medicine, Chonbuk National University) ;
  • Lim, Chae-Wong (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Bumseok (College of Veterinary Medicine, Chonbuk National University) ;
  • Hong, Eui-Ju (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Hyeon-Cheol (College of Veterinary Medicine, Kangwon National University) ;
  • Park, Bae-Keun (College of Veterinary Medicine, Chungnam National University)
  • Received : 2018.04.02
  • Accepted : 2018.10.27
  • Published : 2018.12.31
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Abstract

Present study was performed to describe the morphological and molecular characterization of Toxocara tanuki (Nematoda: Ascaridae) from Korean raccoon dog, Nyctereutes procyonoides koreensis, naturally infected in the Republic of Korea (Korea). Juvenile and adult worms of T. tanuki were recovered in 5 out of 10 raccoon dogs examined and the larval worms were detected in 15 out of 20 muscle samples (75%). Small lateral alae were observed on the cranial end of the body in male and female adults and 2 long spicules (3.0-3.5 mm) were characteristically observed in the posterior end of males. In SEM observation, 18 pairs of proximal precloacal, a precloacal median, a postcloacal median and 5 pairs of postcloacal papillae were uniquely revealed in the posterior portion of males, but the proximal papillae were not shown in the lateral ends of females. Molecular analysis on the 18S rRNA partial DNA sequences was revealed the same finding in both samples, adult worms and muscle larvae, which are closely related to T. tanuki. In conclusion, it was confirmed for the first time that T. tanuki is indigenously distributed, the Korean raccoon dog is acted as the natural definitive host of this nematode in Korea and the morphological characteristics of T. tanuki were shown in specific structure for single postcloacal median papilla in male.

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References

  1. Choi TY, Park CH. Home-range of raccoon dog Nyctereutes procyonoides living in the rural area of Korea. J Ecol Environ 2006; 29: 259-263 (in Korean).
  2. Shin SS, Cha DJ, Cho KO, Cho HS, Choi JO, Cho SH. Arthrostoma miyazakiense (Nematoda: Ancylostomatidae) infection in raccoon dogs of Korea and experimental transmission to dogs. Korean J Parasitol 2007; 45: 121-128. https://doi.org/10.3347/kjp.2007.45.2.121
  3. Shin EH, Park JH, Guk SM, Kim JL, Chai JY. Intestinal helminth infections in feral cats and a raccoon dog on Aphaedo Island, Shinan-gun, with a special note on Gymnophalloides seoi infection in cats. Korean J Parasitol 2009; 47: 189-191. https://doi.org/10.3347/kjp.2009.47.2.189
  4. Eo KY, Kwak D, Kwon OD. Detection of gastrointestinal parasites in raccoon dogs (Nyctereutes procyonoides) in the Seosan reclaimed lands, Korea. J Zoo Wildl Med 2012; 43: 682-684. https://doi.org/10.1638/2012-0053R.1
  5. Sato H, Inaba T, Ihama Y, Kamiya H. Parasitological survey on wild carnivora in north-western Tohoku, Japan. J Vet Med Sci 1999; 61: 1023-1026. https://doi.org/10.1292/jvms.61.1023
  6. Yamaguti S. Studies on the helminth fauna of Japan. Part 35. Mammalian nematodes, II. Jpn J Zool 1940; 21: 409-439.
  7. Li F, Cui J, Wang ZQ, Jiang P. Sensitivity and optimization of artificial digestion in the inspection of meat for Trichinella spiralis. Foodborne Pathog Dis 2010; 7: 879-885. https://doi.org/10.1089/fpd.2009.0445
  8. Rhee JK, Seu YS, Park BK. Isolation and identification of Cryptosporidium from various animals in Korea. I. Prevalence of Cryptosporidium in various animals. Korean J Parasitol 1991; 29: 139-148 (in Korean). https://doi.org/10.3347/kjp.1991.29.2.139
  9. Pozio E, La Rosa G. PCR-derived methods for the identification of Trichinella parasites from animal and human samples. Methods Mol Biol 2003; 216: 299-309.
  10. Sprent JFA. Visceral larva migrans. Aust J Sci 1963; 25: 344-354.
  11. Soulsby EJL. Helminths, Arthropods and Protozoa of Domesticated Animals. 7th ed. London, UK. Bailliere Tindall & Cassell. 1982, pp 150-156.
  12. Cho S, Egami M, Ohnuki H, Saito Y, Chinone S, Shichinohe K, Suganuma M, Akao N. Migration behaviour and pathogenesis of five ascarid nematode species in the Mongolian gerbil Meriones unguiculatus. J Helminthol 2007; 81: 43-47. https://doi.org/10.1017/S0022149X07212118
  13. Sasmal NK, Acharya S, Laha R. Larval migration of Toxocara canis in piglets and transfer of larvae from infected porcine tissue to mice. J Helminthol 2008; 82: 245-249. https://doi.org/10.1017/S0022149X08974344
  14. Beaver PC. Larva migrans. Exp Parasitol 1956; 5: 587-621. https://doi.org/10.1016/0014-4894(56)90032-7
  15. Warren G. Studies on the morphology and taxonomy of the genera Toxocara Stiles, 1905, and Neoascaris Travassos, 1927. Zool Anz 1970; 185: 393-442.
  16. Uga S, Matsuo J, Kimura D, Rai SK, Koshino Y, Igarashi K. Differentiation of Toxocara canis and T. cati eggs by light and scanning electron microscopy. Vet Parasitol 2000; 92: 287-294. https://doi.org/10.1016/S0304-4017(00)00323-X
  17. Anderson RC, Chabaud AG, Willmott S. CIH Keys to the Nematode Parasites of Vertebrates. Farnham Royal, England. Commonwealth Agricultural Bureaux. 1974, pp 1-15.
  18. Gibbons LM, Jacobs DE, Sani RA. Toxocara malaysiensis N. Sp. (Nematoda: Ascaridoidea) from the Domestic Cat (Felis catus Linnaeus, 1758). J Parasitol 2001; 80: 660-665.
  19. Sprent JF. The life history and development of Toxocara cati (Schrank 1788) in the domestic cat. Parasitology 1956; 46: 54-78. https://doi.org/10.1017/S0031182000026342
  20. Barus V, Wiger R, Tenora F, Stanek M. Scanning electron microscopy of the lip denticles of Toxascaris leonina, Toxocara canis and T. cati (Nematoda). Acta Soc Zool Bohem 1979; 43: 3-6.
  21. Uni S, Takada S. The surface structure of sense organs of Toxocara cati (Schrank, 1788) viewed with the scanning electron microscope. Jpn J Parasitol 1975; 24: 365-374.
  22. Fagerholm HP. Systematic implications of male caudal morphology in ascaridoid nematode parasites. Syst Parasitol 1991; 19: 215-229. https://doi.org/10.1007/BF00011888
  23. Hillis DM, Dixon MT. Ribosomal DNA: molecular evolution and phylogenetic inference. Q Rev Biol 1991; 66: 411-453. https://doi.org/10.1086/417338
  24. Chilton NB, Gasser RB, Beveridge I. Differences in a ribosomal DNA sequence of morphologically indistinguishable species within the Hypodontus macropi complex (Nematoda: Strongyloidea). Int J Parasitol 1995; 25: 647-651. https://doi.org/10.1016/0020-7519(94)00171-J
  25. Gasser RB, Monti JR, Bao-Zhen Q, Polderman AM, Nansen P, Chilton NB. A mutation scanning approach for the identification of hookworm species and analysis of population variation. Mol Biochem Parasitol 1998; 92: 303-312. https://doi.org/10.1016/S0166-6851(98)00008-5
  26. Dangoudoubiyam S, Vemulapalli R, Kazacos KR. PCR assays for detection of Baylisascaris procyonis eggs and larvae. J Parasitol 2009; 95: 571-577. https://doi.org/10.1645/GE-1905.1
  27. De Ambrogi M, Aghazadeh M, Hermosilla C, Huber D, Majnaric D, Reljic S, Elson-Riggins J. Occurrence of Baylisascaris transfuga in wild populations of European brown bears (Ursus arctos) as identified by a new PCR method. Vet Parasitol 2011; 179: 272-276. https://doi.org/10.1016/j.vetpar.2011.02.025
  28. Jacobs DE, Zhu X, Gasser RB, Chilton NB. PCR-based methods for identification of potentially zoonotic ascaridoid parasites of the dog, fox and cat. Acta Trop 1997; 68: 191-200. https://doi.org/10.1016/S0001-706X(97)00093-4
  29. Zhu X, Gasser RB, Jacobs DE, Hung GC, Chilton NB. Relationships among some ascaridoid nematodes based on ribosomal DNA sequence data. Parasitol Res 2000; 86: 738-744. https://doi.org/10.1007/PL00008561

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