한국버섯학회지 (Journal of Mushroom)

  • 제16권4호
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  • Pages.272-278
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  • 2018
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  • 1738-0294(pISSN)
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  • 2288-8853(eISSN)
한국버섯학회 (The Korean Society of Mushroom Science)
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원목재배용 표고(Lentinula edodes)의 목질섬유소 분해특성 비교

Analysis of lignocellulose degradation by Oak mushroom (Lentinula edodes)

  • 투고 : 2018.12.04
  • 심사 : 2018.12.20
  • 발행 : 2018.12.31
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초록

본 연구에서는 원목재배용 표고(Lentinula edodes) 품종에 대한 목질섬유소 분해능을 검정하였다. 5개의 국산품종(천백고, 산조 303호, 풍년고, 백화향, 수향고)을 대상으로 malt extract broth(MEB) 배지에 lignin의 첨가에 따른 RBBR(Remazol Brilliant Blue R) 탈색능을 조사한 결과, 산조 303호와 풍년고가 배양 5일째부터 우수한 분해능을 보였다. 섬유소 분해효소인 MnP와 laccase의 활성은 풍년고가 배양 7일째 MnP 활성이 2,809U/mg, laccase 활성이 2,230U/mg으로 나타났고, 산조 303호가 배양 11일째 MnP 활성이 2,673U/mg, laccase 활성이 2,049U/mg으로 최고 활성을 나타났으며, lignin을 첨가하였을 때 효소의 활성이 증가하였다. 산조 303호, 풍년고와 수향고는 배양 5주 만에 filter paper의 분해정도를 육안으로 확인이 가능할 정도로 분해능이 우수하였다.

Lignin degrading enzymes from Lentinula edodes have broad substrate specificities, and therefore can degrade a variety of recalcitrant compounds. In this study, the lignolytic biodegradation was investigated in five different L. edodes fungi (Chunbaegko, Sanjo 303ho, Poongnyunko, Baekhwahyang, and Soohyangko). The fungi were evaluated for their ability to decolorize Remazol Brilliant Blue R (RBBR) in malt extract broth medium. Sanjo 303ho, Poongnyunko, Baekhwahyang, and Soohyangko rapidly decolorized RBBR within 7 days. The activities of manganese peroxidase (MnP) and laccase were determined in the absence and presence of lignin. Poongnyunko displayed the highest ligninolytic activity on day 7 of incubation (2,809 U/mg and 2,230 U/mg for MnP and laccase, respectively).

키워드

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Fig. 1. RBBR decolorization by Lentinula edodes in MEB medium. A: Chunbaegko, B: Sanjo 303ho, C: Poongnyunko, D: Baekhwahyang, E: Soohyangko

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Fig. 2. Extracellular enzymes MnP activities of Lentinula edodes in culture medium. A: Chunbaegko, B: Sanjo 303ho, C: Poongnyunko, D: Baekhwahyang, E: Soohyangko

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Fig. 3. Extracellular enzymes Laccase activities of Lentinula edodes in culture medium. A: Chunbaegko, B: Sanjo 303ho, C: Poongnyunko, D: Baekhwahyang, E: Soohyangko

BSHGBD_2018_v16n4_272_f0004.png 이미지

Fig. 4. Degradation of filter paper by Lentinula edodes. The fungal strains were cultured in yeast nitrogen base broth containing a fragment of filter paper at 28℃, for 5 weeks. A: Chunbaegko, B: Sanjo 303ho, C: Poongnyunko, D: Baekhwahyang, E: Soohyangko

Table 1. Composition of MEB media.

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Table 2. RBBR decolorization by Lentinula edodes in MEB medium as determined by UV spectrophotometer at absorbance 593nm.

BSHGBD_2018_v16n4_272_t0002.png 이미지

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