Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Genetic analysis of Campylobacter jejuni isolates from diarrhea patients in Gyeonggi-do

경기도에서 분리된 Campylobacter jejuni의 유전자 패턴 분석 연구

  • Kim, Woon-Ho (Team of Infectious Disease Investigation, Gyeonggi Province Institute of Health & Environment) ;
  • Choi, Ok-Kyung (Team of Infectious Disease Investigation, Gyeonggi Province Institute of Health & Environment) ;
  • Jeong, Jin-A (Team of Infectious Disease Investigation, Gyeonggi Province Institute of Health & Environment) ;
  • Park, Sung-Hee (Team of Infectious Disease Investigation, Gyeonggi Province Institute of Health & Environment) ;
  • Lee, Yea-Eun (Team of Infectious Disease Investigation, Gyeonggi Province Institute of Health & Environment) ;
  • Park, Gwang-Hee (Team of Infectious Disease Investigation, Gyeonggi Province Institute of Health & Environment) ;
  • Yoon, Mi-Hye (Team of Infectious Disease Investigation, Gyeonggi Province Institute of Health & Environment)
  • 김운호 (경기도보건환경연구원 감염병조사팀) ;
  • 최옥경 (경기도보건환경연구원 감염병조사팀) ;
  • 정진아 (경기도보건환경연구원 감염병조사팀) ;
  • 박성희 (경기도보건환경연구원 감염병조사팀) ;
  • 이예은 (경기도보건환경연구원 감염병조사팀) ;
  • 박광희 (경기도보건환경연구원 감염병조사팀) ;
  • 윤미혜 (경기도보건환경연구원 감염병조사팀)
  • Received : 2017.10.18
  • Accepted : 2018.01.23
  • Published : 2018.03.31
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Abstract

Campylobacter jejuni is an important food-borne pathogen causing gastroenteritis in human. We isolated 208 strains of Campylobacter jejuni from 430 diarrhea patients and food employees with 17 food-poisoning outbreaks between 2014 and 2016 in Gyeonggi area. The strains were tested for genetic relationship and the genotype distribution using PFGE and multiplex-PCR typing. Among the 47 Penner-serotypes known for C. jejuni, it was identified as a genotype consisting of 35 genotypes by multiplex-PCR typing and represented 7 genotypes (HS2, HS4A, HS8, HS15, HS29, HS41, and HS53) in the selected strain. From the PFGE analysis of 11 food-poisoning outbreaks, 5 group of PFGE profile were obtained, and genetic similarity in these clusters ranged from 61.8 to 66.6%. This study examines the genetic diversity of C. jejuni that have been separated in the Gyeonggi area through various genetic analysis methods and identifies the correlation between strains in patients who have been infected with the disease in the future.

캄필로박터 제주니 균(Campylobacter jejuni)은 세균성위장관감염증을 일으키는 수인성식품매개질환의 중요한 원인 균으로 알려져 있다. 2014년부터 2016년까지 경기지역에서 발생된 17번의 식중독에서 캄필로박터 제주니 균에 감염된 430명의 환자와 조리종사자에게서 208건의 균주를 선별하였다. 선별된 균주의 유전적 상관관계와 유전형분포를 확인하기 위하여 PFGE와 multiplex-PCR typing 방법을 사용하여 비교분석 하였다. 47개의 Penner-type으로 구분되는 캄필로박터 제주니 균의 혈청형을 multiplex-PCR typing을 이용하여 35개의 유전형으로 구분할 수 있는 것을 확인하였고 선별된 균주에서 7개의 유전형(HS2, HS4A, HS8, HS15, HS29, HS41, HS53)으로 구분되는 것을 확인하였다. 가장 많은 케이스에서 분리된 유전형은 HS2였고 7건의 식중독케이스에서 확인되었다. PFGE를 통하여 11건의 식중독에서 모두 5개의 그룹으로 분류되었고 그룹간의 유사성은 61.8에서 66.6%였다. 본 연구는 다양한 유전자 분석방법을 통하여 경기도내에서 분리된 캄필로박터 제주니 균의 유전적 다양성을 파악하고 향후 집단발생시 환자의 분리 균주 간의 상관관계 규명하며 캄필로박터 감염증의 발생 및 확산 방지에 필요한 기초자료를 마련하고자 한다.

Keywords

References

  1. Altekruse, S.F., Stern, N.J., Fields, P.I., and Swerdlow, D.L. 1999. Campylobacter jejuni - an emerging foodborne pathogen. Emerg. Infect. Dis. 5, 28-35.
  2. Bacon, D.J., Szymanski, C.M., Burr, D.H., Silver, R.P., Alm, R.A., and Guerry, P. 2001. A phase-variable capsule is involved in virulence of Campylobacter jejuni 81-176. Mol. Microbiol. 40, 769-777. https://doi.org/10.1046/j.1365-2958.2001.02431.x
  3. Cha, I., Kim, N., Nam, J., Choi, E., Chung, G., Kang, Y., and Hong, S. 2014. Genetic diversity of Campylobacter jejuni isolates from Korea and travel-associated cases from east and Southeast Asian countries. Jpn. J. Infect. Dis. 67, 490-494.
  4. EFSA. 2005. Opinion of the Scientific Panel on Biological Hazards on the request from the Commission related to Campylobacter in animals and foodstuffs. EFSA J. 173, 1-10.
  5. Hong, J., Kim, J., Jung, W., Kim, S., Bae, W., Koo, H., Gil, J., Kim, M., Ser, J., and Park, Y.H. 2007. Prevalence and antibiotic resistance of Campylobacter spp. isolated from chicken meat, pork, and beef in Korea, from 2001 to 2006. J. Food Prot. 70, 860-866.
  6. Jeong, J., Lee, J., Lee, H., Lee, S., Kim, S., Ha, J., Yoon, K., and Yoon, Y. 2017. Quantitative microbial risk assessment for Campylobacter foodborne illness in raw beef offal consumption in South Korea. J. Food Prot. 80, 609-618. https://doi.org/10.4315/0362-028X.JFP-16-159
  7. Noormohamed, A. and Fakhr, M.K. 2014. Molecular typing of Campylobacter jejuni and Campylobacter coli isolated from various retail meats by MLST and PFGE. Foods 3, 82-93. https://doi.org/10.3390/foods3010082
  8. Oh, J., Kwon, Y., Wei, B., Jang, H., Lim, S., Kim, C., Jung, S., and Kang, M. 2017. Epidemiological relationships of Campylobacter jejuni strains isolated from humans and chickens in South Korea. J. Microbiol. 55, 13-20. https://doi.org/10.1007/s12275-017-6308-8
  9. Pike, B., Guerry, P., and Poly, F. 2013. Global distribution of Campylobacter jejuni Penner serotypes: A systematic review. PLoS One 8, e67375. https://doi.org/10.1371/journal.pone.0067375
  10. Poly, F., Serichantalergs, O., Kuroiwa, J., Pootong, P., Mason, C., Guerry, P., and Parker, C. T. 2015. Updated Campylobacter jejuni capsule PCR multiplex typing system and its application to clinical isolates from South and Southeast Asia. PLoS One 10, e0144349. https://doi.org/10.1371/journal.pone.0144349
  11. Poly, F., Serichatalergs, O., Schulman, M., Ju, J., Cates, C.N., Kanipes, M., Mason, C., and Guerry, P. 2011. Discrimination of major capsular types of Campylobacter jejuni by multiplex PCR. J. Clin. Microbiol. 49, 1750-1757. https://doi.org/10.1128/JCM.02348-10
  12. Revez, J., Llarena, A.K., Schott, T., Kuusi, M., Hakkinen, M., Kivisto, R., Hanninen, M.L., and Rossi, M. 2014. Genome analysis of Campylobacter jejuni strains isolated from a waterborne outbreak. BMC Genomics 15, 1471-2164.
  13. Samosornsuk, W., Asakura, M., Yoshida, E., Taguchi, T., Eampokalap, B., Chaicumpa, W., and Yamasaki, S. 2015. Isolation and characterization of Campylobacter strains from diarrheal patients in central and suburban Bangkok, Thailand. Jpn. J. Infect. Dis. 68, 209-215. https://doi.org/10.7883/yoken.JJID.2014.229
  14. Szczepanska, B., Andrzejewska, M., Spica, D., and Klawe, J. 2017. Prevalence and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from children and environmental sources in urban and suburban areas. BMC Microbiol. 17, 80. https://doi.org/10.1186/s12866-017-0991-9
  15. Takahashi, M., Koga, M., Yokoyama, K., and Yuki, N. 2005. Epidemiology of Campylobacter jejuni isolated from patients with Guillain-Barre and Fisher syndromes in Japan. J. Clin. Microbiol. 43, 335-339. https://doi.org/10.1128/JCM.43.1.335-339.2005
  16. van den Berg, B., Walgaard, C., Drenthen, J., Fokke, C., Jacobs, B.C., and van Doorn, P.A. 2014. Guillain-Barre syndrome: pathogenesis, diagnosis, treatment and prognosis. Nat. Rev. Neurol. 10, 469-482. https://doi.org/10.1038/nrneurol.2014.121
  17. Wei, B., Cha, S., Kang, M., Roh, J., Seo, H., Yoon, R., and Jang, H. 2014. Antimicrobial susceptibility profiles and molecular typing of Campylobacter jejuni and Campylobacter coli isolates from ducks in South Korea. Appl. Environ. Microbiol. 80, 7604-7610. https://doi.org/10.1128/AEM.02469-14
  18. Zaman, R. 1992. Campylobacter enteritis in Saudi Arabia. Epidemiol. Infect. 108, 51-58. https://doi.org/10.1017/S0950268800049499

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